An increase in glycoprotein concentration on extracellular virions dramatically alters vaccinia virus infectivity and pathogenesis without impacting immunogenicity

The extracellular virion (EV) form of Orthopoxviruses is required for cell-to-cell spread and pathogenesis, and is the target of neutralizing antibodies in the protective immune response. EV have a double envelope that contains several unique proteins that are involved in its intracellular envelopment and/or subsequent infectivity. One of these, F13, is involved in both EV formation and infectivity. Here, we report that replacement of vaccinia virus F13L with the molluscum contagiosum virus homolog, MC021L results in the production of EV particles with significantly increased levels of EV glycoproteins, which correlate with a small plaque phenotype. Using a novel fluorescence-activated virion sorting assay to isolate EV populations based on glycoprotein content we determine that EV containing either higher or lower levels of glycoproteins are less infectious, suggesting that there is an optimal concentration of glycoproteins in the outer envelope that is required for maximal infectivity of EV. This optimal glycoprotein concentration was required for lethality and induction of pathology in a cutaneous model of animal infection, but was not required for induction of a protective immune response. Therefore, our results demonstrate that there is a sensitive balance between glycoprotein incorporation, infectivity, and pathogenesis, and that manipulation of EV glycoprotein levels can produce vaccine vectors in which pathologic side effects are attenuated without a marked diminution in induction of protective immunity.

A bipartite chromatophore transit peptide and N-terminal processing of protein in the Paulinella chromatophore

The cercozoan amoeba Paulinella chromatophora contains photosynthetic organelles - termed chromatophores - that evolved from a cyanobacterium ~100 million years ago, independently from plastids in plants and algae. Despite its more recent origin, at least one third of the chromatophore proteome consists of nucleus-encoded proteins that are imported by an unknown mechanism across the chromatophore double envelope membranes. Chromatophore-targeted proteins fall into two classes. Proteins exceeding 250 amino acids carry a conserved N-terminal sequence extension, termed the 'chromatophore transit peptide' (crTP), that is presumably involved in guiding these proteins into the chromatophore. Short imported proteins do not carry discernable targeting signals. To explore whether the import of protein is accompanied by their N-terminal processing, here we used a mass spectrometry-based approach to determine protein N-termini in Paulinella chromatophora and identified N-termini of 208 chromatophore-localized proteins. Our study revealed extensive N-terminal modifications by acetylation and proteolytic processing in both, the nucleus and chromatophore-encoded fraction of the chromatophore proteome. Mature N-termini of 37 crTP-carrying proteins were identified, of which 30 were cleaved in a common processing region. Our results imply that the crTP mediates trafficking through the Golgi, is bipartite and surprisingly only the N-terminal third ('part 1') becomes cleaved upon import, whereas the rest ('part 2') remains at the mature proteins. In contrast, short imported proteins remain largely unprocessed. Finally, this work sheds light on N-terminal processing of proteins encoded in an evolutionary-early-stage photosynthetic organelle and suggests host-derived post-translationally acting factors involved in dynamic regulation of the chromatophore-encoded chromatophore proteome.

Analysis of indoor environment and insulation performance of residential house with double envelope vacuum insulation panels

Double envelope vacuum insulation panels (VIPs) have a possibility to significantly increase the service lifetime. In this paper, double envelope VIPs were produced and installed in the residential house. The performance of installed VIPs was evaluated by using the measuring data of heat flux meter. In addition, the total energy, the heating load and the indoor thermal environment of this house were measured and analysed. The average heating load and the average temperature difference between room temperature and ambient air temperature on the representative day was 2.49 kW and 29.9 oC, respectively. The heat loss coefficient per floor area was estimated as 0.69 W/(m2K) and it was almost the same as the value calculated at the time of design. The result of indoor environment measurement showed that the room temperature was maintained at around 20 oC and PMV was -0.5 oC or higher although the outside air temperature fluctuated between -5 oC and -10 oC. The effective thermal conductivities of double envelop VIPs were all estimated as 0.01 W/(mK) or less. It is considered that the insulation performance of the vacuum insulation panels is maintained.

In the service of secrecy: An enveloped history of priority, proof and patents

This article is about an everyday paper object: an envelope. However, as opposed to most other flat paper containers, the enveloppe Soleau can only be bought from L’Institut national de la propriété industrielle (INPI) in Paris. At the cost of €15 you get a perforated, double-compartment envelope allowing you to constitute proof of creation and assign a precise date to your idea or project. But the enveloppe Soleau is something much more than just a simple and cheap way by which you can prove priority in any creative domain. It is a material footprint anchored to centuries of practices associated with disclosure and secrecy, a gateway into the infrastructure of the intellectual property system and its complicated relationship to the forms of knowledge it purports to hold. The purpose of this article is to consider the making of the enveloppe Soleau as a bureaucratic document, a material device performing a particular kind of legal paperwork. In four different vignettes, the article tracks the material becoming of the enveloppe Soleau as an evidentiary receptacle, beginning by going back to early modern practices of secrecy and priority, continuing with its consolidation in two patents (from 1910 and 1911) to the inventor Eugène Soleau (1852–1929), and ending up, in 2016, dematerialized in the e-Soleau. As a bureaucratic document, the enveloppe Soleau shows just how much work a mundane paper object can perform, navigating a particular materiality (a patented double envelope); formalized processes of proof (where perforations have legal significance); the practices of double archiving (in an institution and with the individual) and strict temporal limitations (a decade). Ultimately, the enveloppe Soleau travels between the material and immaterial, between private and public, between secrecy and disclosure, but also between what we perceive of as the outside and inside of the intellectual property system.

Thermal and Geometrical Assessment of Parabolic Trough Collector Mounted Double Evacuated Novel Receiver Tube System

This paper particularly aims to highlight the necessity of optimal geometric design considerations of a parabolic trough collector (PTC) mounted novel receiver tube in view of efficient operation and high-end performance. Many investigations, analysis, and validation have been done in this regard as solar energy based PTC now a commercially mature technology acknowledges a variety of role in the form of power generation and other thermal applications. This article identifies the optimal rim angle corresponding to its tube size as required for high exergetic gains. Almost six receiver tubes, distinct in terms of dimensions and number of covers are compared for their best results to be mounted on adequate geometry with different rim angle (40°, 80°, and 120°). A significant variation of flow rate (i.e. 16 to 216 litre/hr) and inlet fluid temperature (i.e. 323 K, 423 K, 523 K, 623 K, and 723 K) has been extensively detailed about high energy and exergy retrieval from the system. The study reports that all the favorable results are found with the receiver tube having a diameter of 0.027 m and a double envelope, compared to other design considerations. Results show that as the flow rate increases energy efficiency also increases up to some extent along with increasing receiver tube temperature. The highest energy and exergy efficiency as reported to be 79.4% and 47% respectively with 80o being the optimal rim angle for a 5.7 m wide parabolic aperture.

A three-dimensional thermal study of a mercury discharge lamp with double envelope for different orientations

This paper is devoted to study the dynamics of a discharge lamp in different position. As an example of application, we chose the mercury lamp. For this, we realized a three-dimensional model, steady state. After the validation of this model, we used it to reproduce the influence of some parameters that have appeared on major transport phenomena of mass and energy in studying the lamp operating in different positions. Indeed, the pressure and the orientation of the lamp are modified. The effect of convective transport and the accumulation of mercury behind the electrodes are studied.