Pengaruh Suhu Wet Rendering yang Berbeda terhadap Karakteristik Ekstrak Kasar Minyak Ikan Lele (Clarias sp.)

Pengolahan minyak isi perut ikan lele dapat meningkatkan nilai ekonomi limbah ikan lele. Wet rendering merupakan metode yang sering digunakan untuk mengekstrak minyak ikan. Metode ini menggunakan panas dan air untuk membantu proses ekstraksi. Perlakuan suhu bertujuan untuk menggumpalkan protein dan merusak membran sel sehingga minyak dapat terekstrak keluar. Adanya air memudahkan pemisahan karena minyak yang terekstrak akan mengapung pada permukaan. Penggunaan suhu ekstraksi berpengaruh terhadap kualitas minyak ikan yang dihasilkan. Suhu ekstraksi yang tinggi menyebabkan penurunan kualitas minyak akibat terjadinya reaksi oksidasi. Tujuan penelitian ini adalah untuk mengetahui pengaruh suhu ekstraksi wet rendering terhadap karakteristik ekstrak kasar minyak isi perut ikan lele dan mengetahui suhu ekstraksi optimum berdasarkan karakteristik ekstrak minyak isi perut ikan lele yang dihasilkan. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor yaitu perbedaan suhu ekstraksi wet rendering (80 °C, 90 °C, dan 100 °C) dengan tiga kali pengulangan. Hasil penelitian menunjukkan bahwa semakin tinggi suhu ekstraksi menghasilkan minyak isi perut ikan lele dengan rendemen yang semakin meningkat. Namun, kualitas minyak ikan semakin menurun yang ditunjukkan dengan meningkatnya asam lemak bebas dan angka peroksida, yang berakibat pada penurunan nilai organoleptik, perubahan profil asam lemak dan slip melting point. Suhu 100 °C merupakan suhu ekstraksi terbaik dengan nilai organoleptik 7,87; rendemen 8,57%; slip melting point 37,53 °C; bilangan iod 16,01%; total SFA 42,9%; MUFA 31,8%; PUFA 11,51%; angka asam lemak bebas 2,00%; kadar air 0,56%; dan bilangan peroksida 7,26 meq/kg.

Preliminary Evaluation of Supercritical Carbon Dioxide Extracted Dabai Pulp Oleoresin as a New Alternative Fat

There has been growing interest among food scientists in producing a toxin-free fat as an end product with varying physical or nutritional properties of interest to the food industry. Oleoresin is a rich source of bioactive compounds which consumers can easily add to a large variety of food. Dabai (Canarium odontophyllum) pulp oleoresin (DPL) was extracted using supercritical carbon dioxide (SC-CO2) extraction, a green extraction technology. This study investigates the quality of SC-CO2 extracted DPL in discovering its potential as a new alternative fat. The extraction experiment was carried out at a pressure of 40 MPa and a temperature of 40 °C. DPL is a saturated fatty acid (SFA)-rich fat due to its high SFA composition (47.72 ± 0.01%). In addition, the low content of peroxide value (PV) (5.60 ± 0.09 mEq/kg) and free fatty acids (FFA) (3.40 ± 0.03%) indicate the quality and stability of DPL for various applications besides food consumption. DPL also has a low slip melting point (SMP) (20.20 ± 0.03 °C), and HPLC-FID revealed that DPL contained 0.13 ± 0.02 mg/100 g of vitamin E (α-tocopherol), indicating its potential application as a solid fat with a bioactive compound. This present work demonstrates the possible prospect of DPL in the formulation of end products for food industries.

Synthesis of margarine fat from sesame oil and palm stearin by chemical interesterification

The production of margarine fat is not only intended to be free from Trans Fatty Acid (TFA) but is also expected to have a higher quality from a nutritional aspect. In this research, margarine fat from sesame oil (SO) and palm stearin (PS) by a chemical interesterification was synthesized. Chemical interesterification is one of the processes used to modify the physico-chemical characteristics of oils and fats. An attempt to chemical-restructure palm stearin and sesame oil to form margarine fat which is suitable for margarine was investigated using sodium methoxide as a catalyst. The effect ratio of PS/SO in color, slip melting point, solid fat index, texture and triacylglycerols (TAGS) profile of margarine fat were studied in research. This research was conducted by three major stages; characterization of sesame oil and palm stearin, synthesis of margarine fat by physical blending and chemical interesterification, and characterization of margarine fat. This study used a Completely Randomized Design (CRD) with one factor, namely the ratio of sesame oil and palm stearin. Margarine fat produced with different variations of the raw material concentration (% w/w SO:PS = 30:70; 40: 60; 50:50, 60:40, and 70:30). Chemical interesterification caused: rearrangement of triacylglycerols, reduction of S3 and U3 and increase in S2U and U2S type TAGs content of all blend, resulting in lowering of melting point, solid fat index and increase texture. Margarine fat in the % ratio of SO:PS = 60:40, has a similar characteristic (texture, slip melting point, solid fat index, and TAGS profiles) which margarine commercial, so has the potential to be developed in the manufacture margarine industry

Lipase-catalyzed transesterification of medium-long-medium structured lipid (MLM-SL) using palm olein and tricaprylin in packed-bed reactor (PBR)

Lipase-catalyzed transesterification between refined bleached deodorized palm olein (RBDO) and tricaprylin to produce medium-long-medium structured lipid (MLM-SL) in a packed bed reactor (PBR) has been investigated. A specific sn-1,3 commercial Lipozyme TL IM was used as biocatalyst. Within this study, the progress of transesterification was monitored especially for triacylglycerol (TAG) formation with equivalent carbon number (ECN) of 32, presumably 1,3-dicapryoyl-2-oleoyl-sn-glycerol (COC). Transesterification conditions investigated were residence times (i.e., 15, 30, and 60 min) and enzyme loadings (2.0 and 4.5 g). The highest yield of ECN 32 (13%) and transesterification degree (71%) were obtained at residence time of 15 mins for both enzyme loadings. Longer residence time seemed to facilitate lipid hydrolysis over transesterification. This was indicated by the number of peaks appearing in the high-performance liquid chromatography (HPLC) chromatograms and the reduction of fat slip melting point (SMP). Additionally, the highest productivity was obtained at 2.0 g enzyme loading. Conclusively, this study has demonstrated the potential use of packed-bed reactor with immobilized Lipozyme TL IM for continuous synthesis of MLM-SLs especially TAG with ECN32.

Lipase-mediated interesterification of oils and fats for the preparation of bakery and confectionery fats

Bakery and confectionary fats were prepared by enzymatic interesterification of sal fat with palm stearin and palm mid fraction blends in various ratios. Slip melting point, free fatty acids, fatty acid composition, solid fat content and triglyceride composition were determined. Fatty acid composition revealed that the blends were rich in palmitic (13.9-58.5%), stearic (7.7-36.7%) and oleic (25.2-39.9%) with no trans fatty acids. Blends of sal:PSt (50:50), sal:PMF (50:50 and 25:75) showed high solid fat content at 20 and 25 °C with short melting range. After interesterification, plasticity of products increased, which were comparable with commercial bakery fat. Some of the blends alone showed short melting profile like cocoa butter. Interesterification produced significant alteration in the triacylglycerol composition of the blends studied. Blends and the interesterified products prepared showed favorable characteristics with no transfats and hence could be used in the place of commercial bakery and confectionary fats.

Karakteristik Fisik Shortening Hasil Interesterifikasi Kimiawi Campuran Terner Minyak Biji Karet, Minyak Ikan Nila, dan Palm Stearin

<p><em>Shortening </em>merupakan produk olahan lemak dan minyak yang berfungsi sebagai pembentuk pori dan pelembut pada pembuatan roti. <em>Shortening </em>diproduksi dari campuran stearin sawit (PS), minyak biji karet (RSO) dan minyak ikan (FO), pencampuran fraksi padat dan cair dilakukan dengan tujuan mendapatkan titik leleh dan konsistensi yang diinginkan sesuai dengan shortening komersial. Rancangan percobaan menggunakan rancangan acak kelompok (RAK) menggunakan sepuluh campuran terner lemak dengan rasio dan metode berbeda, masing-masing PS / RSO/ FO (50/30/20, 60/35/5, 70/15/15, 80/10/10 dan 90/5/5) (% bb) dengan metode <em>blending</em>/<em>non interesterification </em>(NIE) dan interesterifikasi kimiawi/ <em>chemical interesterification </em>(CIE). Setiap perlakuan dilakukan ulangan sebanyak 3 kali. Karakteristik fisik lemak yang diuji meliputi, titik leleh, indeks total padatan lemak, komposisi asam lemak, warna, dan tekstur. Seluruh hasil analisa dari semua campuran dilakukan uji statistik menggunakan <em>software </em>SPSS versi 22. Hasil uji statistik menunjukkan perbedaan rasio dan metode yang digunakan secara signifikan berbeda nyata (P&lt;0,05). Campuran interesterifikasi kimiawi dengan rasio (CIE) 80/10/10 dan 90/5/5 merupakan perlakuan yang menghasilkan sifat fisik yang paling mendekati <em>shortening </em>komersial dengan karakteristik meliputi <em>slip melting point </em>pada kisaran (40,38-42,54°C), titik leleh (47,33-50,46°C), indeks kepadatan lemak (17,92-31,63%), kecerahan (86,01-87,64), tekstur (421,75-872,25 gf/cm2).</p><p> </p><p><strong>Physica</strong><strong>l </strong><strong>Characteristic</strong><strong>s Of Shortening Results Of </strong><strong>C</strong><strong>hemical</strong> <strong>Interesterifications On Terner Mixed Seed Oil Rubber, Oil Fish, And</strong> <strong>Palm Stearin</strong></p><p>Shortening is produced from a mixture of palm stearin (PS), rubber seed oil (RSO) and fish oil (FO). The experimental design used ten fat ternary mixtures with different ratios and methods, each PS / RSO / FO (50/30/20, 60/35/5, 70/15/15, 80/10/10 and 90 / 5/5) (% bb) with blending (NIE) and chemical interesterification (CIE) methods. The physical characteristics of the fat tested include, melting point, total solid fat index, fatty acid composition, color, and texture. The experimental design used was a randomized block design. All analysis results of all mixtures were carried out using a SPSS version 22 statistical test. The statistical test results showed that the ratio and method used were significantly different (P &lt;0.05). Chemical interesterification mixture with a ratio (CIE) 80/10/10 and 90/5/5 is the treatment that produces the physical properties that are closest to commercial shortening with characteristics including slip melting point in the range of (40.38-42.54 ° C), melting point (47.33-50.46 ° C), solid fat index (17.92-31.63%), Lightness (86.01-87.64), texture (421.75-872.25 gf / cm2).</p>

The synthesis of medium-long-medium structured lipid (MLM-SL) by lipase-catalyzed transesterification using palm olein and tricaprylin in packed-bed reactor (PBR)

Lipase-catalyzed transesterification between refined bleached deodorized palm olein (RBDO) and tricaprylin to produce medium-long-medium structured lipid (MLM-SL) in a packed bed reactor has been investigated. A specific sn -1,3 commercial Lipozyme TL IM was used as biocatalyst. Within this study, the progress of transesterification was monitored especially for triacylglycerol (TAG) formation with equivalent carbon number (ECN) of 32, presumably 1,3-dicapryoyl-2-oleoyl- sn -glycerol (COC). Transesterification conditions investigated were residence times ( i.e ., 15, 30, and 60 min) and enzyme loadings (2 and 4.5 g). The highest yield of ECN 32 (13%) and transesterification degree (71%) were obtained at residence time of 15 mins for both enzyme loadings. Longer residence time seemed to facilitate lipid hydrolysis over transesterification. This was indicated by the number of peaks appearing in the high-performance liquid chromatography (HPLC) chromatograms and the reduction of of fat slip melting point (SMP). Additionally, at 4.5 enzyme loading the highest productivity was obtained for one-cycle reaction. Conclusively, this study has demonstrated the potential use of packed-bed reactor with immobilized Lipozyme TL IM for continuous synthesis of MLM-SLs especially TAG ECN32.

Characteristics of rambutan (Nephelium lappaceum L.) seed fat fractions and their potential application as cocoa butter improver

The utilization of rambutan seed (Nephelium Lappaceum L.) to produce fat (RSF) and its fractionation could be one of the solutions for better waste management and for ensuring its sustainable utilization. In this study, RSF was fractionated by two-stage acetone fractionation and their physicochemical properties such as fatty acid compositions, iodine value (IV), free fatty acid (FFA), slip melting point (SMP), and solid fat content (SFC) were investigated. The solid fraction-III (F2-S) exhibited the highest SMP (49.03°C) and lowest IV (27.57 g I2/100 g). The major fatty acids in all solid fractions were stearic (15.1- 21.6%), oleic (25.0-35.5%), and arachidic (42.7-46.9%) acids. The SFC of F2-S at 20°C (78.57%) and 35°C (22.95%) were found to be higher than solid fraction-I (F1-S), indicating a harder solid fraction. This study revealed that by performing fractionation of RSF, a cocoa butter improvers (CBI) could be prepared by blending them with other fats that have the potential to be utilized in chocolate manufacturing in tropical countries

The Characteristics of Striped Catfish Oil (Pangasius hypophthalmus) Extracted by Dry Rendering Method at Different Temperatures

<p>Striped catfish (<em>Pangasius hypophthalmus</em>) is a high-fat fish compared to other freshwater fish like snakehead fish and carp. Striped catfish oil contains unsaturated and polyunsaturated fatty acids that are beneficial for health. The quality of catfish oil is affected by the extraction method, especially the preliminary heating temperature for the extraction. This study aimed to determine the effect of different heating temperatures on the characteristics of catfish oil and find the best heating temperature in the dry rendering process. This study used a completely randomized design with three different heating temperatures (80°C, 100°C and 120°C). The result showed that the extraction at various temperatures was significantly different on the yield, moisture content, peroxide value, iodine value, free fatty acids value and slip melting point, but not significantly different in sensory properties. A higher heating temperature could increase the yield percentage, free fatty acids values, peroxide values, iodine values, except to the moisture contents, slip melting point and fatty acid profiles. The best temperature was 100°C for 20 minutes with 9.09% yield, 1.44% moisture content, 1.72% free fatty acid, 15.82% iodine value and sensory of 7.65&lt;µ&lt;8.15. Based on the results, it can be concluded that the dry rendering temperature affects the characteristics of the catfish oil.</p>