The Attenuated Total Reflection (ATR) technique coupled with a microscope allows analysis of specimens without any preparation, spatially correlated with the specimenâs morphology. These characteristics make micro-ATR systems very useful for studying gemstones and in particular amber samples. Indeed, in this report the micro-ATR technique was used to characterize three Burmite samples, as case studies. Particularly, focusing the ATR crystal spot on the amber surface where a difference in the UV reaction was previously detected, thereby relative differences in the IR spectrum could be analyzed. Here we present a methodology combining the UV imaging technique with the micro-ATR/FTIR analyses, in order to correlate the fluorescence information with the molecular vibrational modes of amber and their relative spatial distribution. Finally, this work is intended as an initial methodology study and part of the amber characterization project, focused on characterizing the Burmese Amber collection of the Peretti Museum Foundation, from several disciplines (i.e. Palaeoentomology, vibrational spectroscopy, neutron tomography, etc).