The Hologenome of Haliclona fulva (Porifera, Demospongiae) Reveals an Abundant and Diverse Viral Community

Viruses are among the most abundant biological entities in the ocean, largely responsible of modulating nutrients fluxes and influencing microbial composition and functioning. In marine invertebrate holobionts like sponges and their associated microbiomes, little is known about virome composition. Here, we characterized the Haliclona fulva hologenome, an encrusting low-microbial abundance sponge found across the Western Mediterranean Sea (35–40 m of depth) producer of a large metabolic repertoire of bioactive compounds and harboring a distinct and stable associated microbiome. Assembled contigs from shotgun metagenome sequences obtained from H. fulva specimens were comprehensively analyzed regarding taxonomic and functional content revealing its remarkable and abundant viral community dominated by single-stranded DNA (ssDNA) virus. Viral families consistently detected in contigs are Circoviridae, Phycodnaviridae, Poxviridae, Herelleviridae, Mimiviridae, Microviridae, and notably the first reported encounter of Nanoviridae and Genomoviridae in Porifera, expanding their known host range. The relative abundance of inferred bacteriophages/prophages was low, suggesting that the prokaryotic community in this sponge has a limited host range and susceptibility. H. fulva showed a distinct viral composition supporting the general proposition of specific and coevolving viromes in marine holobionts.

ACCELERATED CELL DEATH 6 Acts on Natural Leaf Senescence and Nitrogen Fluxes in Arabidopsis

As the last step of leaf development, senescence is a molecular process involving cell death mechanism. Leaf senescence is trigged by both internal age-dependent factors and environmental stresses. It must be tightly regulated for the plant to adopt a proper response to environmental variation and to allow the plant to recycle nutrients stored in senescing organs. However, little is known about factors that regulate both nutrients fluxes and plant senescence. Taking advantage of variation for natural leaf senescence between Arabidopsis thaliana accessions, Col-0 and Ct-1, we did a fine mapping of a quantitative trait loci for leaf senescence and identified ACCELERATED CELL DEATH 6 (ACD6) as the causal gene. Using two near-isogeneic lines, differing solely around the ACD6 locus, we showed that ACD6 regulates rosette growth, leaf chlorophyll content, as well as leaf nitrogen and carbon percentages. To unravel the role of ACD6 in N remobilization, the two isogenic lines and acd6 mutant were grown and labeled with 15N at the vegetative stage in order to determine 15N partitioning between plant organs at harvest. Results showed that N remobilization efficiency was significantly lower in all the genotypes with lower ACD6 activity irrespective of plant growth and productivity. Measurement of N uptake at vegetative and reproductive stages revealed that ACD6 did not modify N uptake efficiency but enhanced nitrogen translocation from root to silique. In this study, we have evidenced a new role of ACD6 in regulating both sequential and monocarpic senescences and disrupting the balance between N remobilization and N uptake that is required for a good seed filling.

Nutrients Load Estimation in a Regulated Streamflow Estuary: The São Francisco Estuary (NE/Brazil)

This study aimed to estimate the nutrients concentration variability in the São Francisco estuary from observed data during the summer and winter seasons of 2014 and to assess the influence of outflow discharges, circulation and precipitation on nutrients fluxes exchange. The marginal difference in the streamflow (1,160 m3/s - summer; 1,260 m3/s - winter) reflected the small role of the river discharge on the variability of the nutrients load. The increase in the sediments load from February (13189.70 T/month) to July (36088.56 T/month) revealed that high precipitation (153.6 mm - winter; 37.6 mm - summer) and internal circulation might have contributed to enhancing the sediment budge towards the estuary mouth. The strong current velocity in July (~0.9 m/s) revealed more potential to export estuarine waters towards the coast when compared to ~0.7 m/s (February). The concentrations and nutrients load were higher during the winter season led by phosphate (3.70 μgL-1) and NID (83.64 μg L-1), against (1.38 μgL-1 and 30.70 μgL-1 - summer), except for silicates with 4.20 mgL-1 (summer) and 3.59 mgL-1 (winter). Despite the active control of outflows, the internal circulation, followed by local precipitation, are considered the main mechanisms behind the increased nutrients load within the estuary.

Distribution of carbon and nutrients and fluxes of mineral nitrogen after clear-felling a Pinusradiata plantation

The effects of clear-felling and slash removal on the distribution of organic matter and nutrients, fluxes of mineral N, and soil water and temperature were studied in a 37-year-old Pinusradiata D. Don plantation, on a sandy Podzol in southeastern Australia. Slash, litter, and the top 30 cm of soil combined contained 1957 kg N•ha−1, of which slash and litter contained 12 and 25%, respectively. Therefore, loss of slash and litter due to burning or other intensive site preparation practices would substantially reduce the N capital at the site. During the first 18 months after clear-felling, soil water content in the clear-felled area was up to 50% higher than in the uncut plantation, but there were only minor differences in soil temperature. Slash removal decreased the water content of litter, but had little effect on the water content or temperature of the soil. In the uncut plantation, N mineralized in litter and soil was completely taken up by the trees. Following clear-felling, rates of N mineralization increased in litter after 4 months, and in soil after 12 months, but changes were less pronounced with slash removal. After clear-felling, increased mineralization and the absence of trees (no uptake) led to increased concentrations of mineral N in both litter and soil, 64–76% of which was leached below the 30 cm soil depth prior to replanting. Despite leaching, concentrations of mineral N after clear-felling remained higher than those in the uncut plantation for at least 3 years.