Pulse Voltage Electrical Stimulation for Bacterial Inactivation and Wound Healing in Mice with Diabetes

Background: Treatment of wounds in diabetes often gets less than perfect healing. One of the reasons for the difficulty in treating wounds in diabetes is the growth of aerobic and anaerobic bacteria. This study aims to determine the pulse voltage and treatment time that can optimally inactivate bacteria, and their effect on wound healing in mice suffering from diabetes. Methods: The study used electrical stimulation with a direct voltage of 10 volts given a pulse voltage of 50-80 volts, a width of 50 µs, and the number of pulses of 65 per second. The research samples were Staphylococcus aureus (S. aureus) and Pseudo-monas aeruginosa (P. aeruginosa) bacteria that grew on beef and mice (Mus musculus) with diabetes. The treatment for S. aureus and P. aeruginosa bacteria was carried out using a pulse voltage of 50-80 volts for 5-15 min/day and repeated for 3 days. Meanwhile, treatment of mice wounds was carried out with a pulse voltage of 80 volts for 15 min/day and repeated for 7 days. Results: The results showed that treatment with a pulse voltage of 50-80 volts and a treatment time of 5-15 min significantly reduced the number of S. aureus and P. aeruginosa bacteria in beef (p£0.05). Treatment with a pulse voltage of 80 volts for 15 min made beef free from bacteria. Meanwhile, treatment with a pulse voltage of 80 volts for 15 min per day for seven days resulted in the wound state of three mice in the maturation phase and two mice in the proliferation phase on day 8 with an average wound area of 0.108 cm 2. Conclusion: The treatment with a pulse voltage of 80 volts for 15 min made the beef sterile, the mice wounds healed quickly, and the mice not stressed. The higher the blood glucose level, the slower the wound healing process.

DEVELOPMENT OF BEHAVIORAL MODEL FOR SWITCHING VOLTAGE REGULATOR MICROCIRCUIT 1290EF1

The issue of developing a behavioral model of pulse voltage converter IC is considered on the example of a microcircuit of domestic production 1290EF1. In order to verify the adequacy of the model, taking into account its frequency characteristics, recommendations and basic requirements are given when performing a full-scale and numerical experiment to determine the frequency characteristics of such type of nonlinear dynamic objects.

MATCHING OF COMPATIBLE WORK OF SHORT HIGH-VOLTAGE PULSES OF TENSION GENERATOR AND WATER TREATMENT CHAMBER BY DINT OF PULSE BARRIER DISCHARGE

A technique for calculating the parameters of a magnetic switch as an element of a generator of short high-voltage pulses of tension to coordinate its compatible work with a water treatment chamber by dint of pulse barrier discharge is shown. The expediency and efficiency of using such a switch as an element that, by shunting, the discharge chamber, discharges the barrier to the arrival of the next voltage pulse has been confirmed. It is proved that with the accepted geometrical dimensions of the discharge chamber and the amplitude of the pulse voltage, provided that the magnetic switch is present that it is possible to increase the practical use of electricity by ~ 40% due to that which was accumulated in the dielectric barrier in one discharge. Ref.10, fig. 5.

The experimental results of the geophysical pulse voltage generator

The paper is devoted to the development process of the geophysical pulse voltage generator. The peculiarity of the generator lies in the non-specific purpose of this type of construction. Its main function is the controlled effect on the active faults of seismically dangerous zones. The results of the field experiment with the constructed device from 2018 to 2020 are presented. During the experiments, estimations and theoretical analysis we have obtained the seismic noise recorded by means of molecular electronic instruments and determined the periods of the electrical pulses. Operational parameters of the generator were identified on the basis of experiment results. The software and hardware elements of the system were upgraded and, as a result, a new version of the geophysical pulse voltage generator was developed.

Effects of Pulse Voltage Duration on Open–Close Dynamic Characteristics of Solenoid Screw-In Cartridge Valves

The hydraulic high-speed on/off valve (HSV)—the critical core component of digital hydraulic technology—has a special structural design and manufacture due to its fast opening and closing, which results in high prices and maintenance costs. The solenoid screw-in cartridge valve (SCV) is widely used in the hydraulic industry because of its merits, such as mature technology, reliable quality, and low cost. The contribution of this study is to replace the high-speed on/off valve with the SCV in some areas of application by introducing positive and negative pulse voltage control for the coil of the SCV, which only modifies the control circuit and needs no change in structure. Based on the analysis of the structure of the SCV, the simulation model was developed in AMESim and validated by experiments to investigate the effects of the pulse voltage duration on the open–close dynamic characteristics and find the optimal pulse voltage duration, so that the SCV can open or close in the shortest time to reduce energy loss as far as possible. The simulation results showed that the positive and negative pulse voltage could quicken the rising or declining speed of the coil current and dramatically decrease the opening and closing delay time. By the experimental comparison with the original control method, the opening time of the SCV decreased from 30 ms to 13 ms, and the closing time was reduced from 139 ms to 14 ms.