Pulse Voltage Electrical Stimulation for Bacterial Inactivation and Wound Healing in Mice with Diabetes

2022 ◽  
Author(s):  
Mokhamad Tirono ◽  
Farid Samsu Hananto ◽  
Ahmad Abtokhi
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Treatment Time ◽  
Healing Process ◽  
Anaerobic Bacteria ◽  
Pulse Voltage ◽  
Wound Healing Process ◽  
Bacterial Inactivation ◽  
Wound Area ◽  
Treatment Of Wounds

Background: Treatment of wounds in diabetes often gets less than perfect healing. One of the reasons for the difficulty in treating wounds in diabetes is the growth of aerobic and anaerobic bacteria. This study aims to determine the pulse voltage and treatment time that can optimally inactivate bacteria, and their effect on wound healing in mice suffering from diabetes. Methods: The study used electrical stimulation with a direct voltage of 10 volts given a pulse voltage of 50-80 volts, a width of 50 µs, and the number of pulses of 65 per second. The research samples were Staphylococcus aureus (S. aureus) and Pseudo-monas aeruginosa (P. aeruginosa) bacteria that grew on beef and mice (Mus musculus) with diabetes. The treatment for S. aureus and P. aeruginosa bacteria was carried out using a pulse voltage of 50-80 volts for 5-15 min/day and repeated for 3 days. Meanwhile, treatment of mice wounds was carried out with a pulse voltage of 80 volts for 15 min/day and repeated for 7 days. Results: The results showed that treatment with a pulse voltage of 50-80 volts and a treatment time of 5-15 min significantly reduced the number of S. aureus and P. aeruginosa bacteria in beef (p£0.05). Treatment with a pulse voltage of 80 volts for 15 min made beef free from bacteria. Meanwhile, treatment with a pulse voltage of 80 volts for 15 min per day for seven days resulted in the wound state of three mice in the maturation phase and two mice in the proliferation phase on day 8 with an average wound area of 0.108 cm 2. Conclusion: The treatment with a pulse voltage of 80 volts for 15 min made the beef sterile, the mice wounds healed quickly, and the mice not stressed. The higher the blood glucose level, the slower the wound healing process.  

scholarly journals Jatropha Neopauciflora Pax Latex Exhibits Wound-Healing Effect in Normal and Diabetic Mice

2021 ◽  
Vol 26 ◽  
pp. 2515690X2098676
Author(s):  
Ana Bertha Hernandez-Hernandez ◽  
Francisco Javier Alarcon-Aguilar ◽  
Mario Garcia-Lorenzana ◽  
Marco Aurelio Rodriguez-Monroy ◽  
Maria Margarita Canales-Martinez
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Wound Area ◽  
Oral Infections ◽  
Patients With Diabetes ◽  
Exclusion Chromatography ◽  
Wound Healing Effect ◽  
Molecular Exclusion Chromatography

Jatropha neopauciflora is an endemic species of Mexico. Its latex is used to treat wounds, scarring, oral infections, and loose teeth. To date, there are no studies that validate at a morphological level a wound-healing use in diabetes. The present research aimed to evaluate the wound-healing capacity of the latex of J. neopauciflora in the skin of healthy and streptozotocin-induced diabetic mice. Also, a chemical analysis of the latex through molecular exclusion chromatography and HPLC were performed. Male mice ( Mus musculus) of 7-week-old CD1 strain were used. Groups of healthy and diabetic mice were formed. A longitudinal cut of 1 cm was performed on the depilated skin. All treatments were topically applied to the wound area twice a day for ten days. At the end of the experiments, the skin sections were obtained from the wound area and stained with Hematoxylin-Eosin. Then we counted the number of active fibroblasts in all the experimental groups. In normal mice, the latex accelerated the wound-healing process and decreased the number of active fibroblasts, similarly to Recoveron. In diabetic mice, the latex and Recoveron increased the number of active fibroblasts. In normal and diabetic mice, a thin and orderly epidermis was observed. Molecular exclusion chromatography exhibited 58 fractions, 14 of which were subjected to HPLC, to detect catechin, a flavonoid with antioxidant, antimicrobial, and anti-inflammatory properties. J. neopauciflora latex can be useful for wound treatment in patients with diabetes mellitus because it accelerates and promotes the wound-healing process.

scholarly journals Polymer-Based Scaffolds Loaded with Aloe vera Extract for the Treatment of Wounds

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 961
Author(s):  
Sibusiso Alven ◽  
Vuyolwethu Khwaza ◽  
Opeoluwa O. Oyedeji ◽  
Blessing A. Aderibigbe
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Mechanical Performance ◽  
Healing Process ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
Biological Properties ◽  
Wound Management ◽  
Wound Healing Process ◽  
Treatment Of Wounds

The treatment of wounds is one challenging biomedical field due to delayed wound healing common in chronic wounds. Several factors delay wound healing, including microbial infections, malnutrition, underlying physiological conditions, etc. Most of the currently used wound dressing materials suffer from poor antimicrobial properties, poor biodegradability and biocompatibility, and weak mechanical performance. Plant extracts, such as Aloe vera, have attracted significant attention in wound management because of their interesting biological properties. Aloe vera is composed of essential constituents beneficial for the wound healing process, such as amino acids, vitamins C and E, and zinc. Aloe vera influences numerous factors that are involved in wound healing and stimulates accelerated healing. This review reports the therapeutic outcomes of aloe vera extract-loaded polymer-based scaffolds in wound management.

Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

2020 ◽  
Vol 29 (9) ◽  
pp. 488-495
Author(s):  
Amene Nikgoftar Fathi ◽  
Mohammad Hassan Sakhaie ◽  
Sepehr Babaei ◽  
Soroush Babaei ◽  
Fateme Slimabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Wound Area ◽  
Small Vessels ◽  
Histological Sections ◽  
Therapeutic Benefits ◽  
Wide Range

Objective: To assess the effect of bromelain on different aspects of the wound healing process in type 1 diabetic rats. Method: In this study, 112 streptozocin-diabetic (type 1) male Wistar rats were euthanised; 28 each on days three, five, seven and 15, after a wound incision had been made. To estimate changes in a number of different cellular and tissue elements, histological sections were provided from all wound areas and stained with haematoxylin and eosin. Some 1.056mm2 of total wound area from all specimens were evaluated, by assessment of 4200 microscope photos provided from all histological sections, by stereological methods. A biomechanical test of each wound area was performed with an extensometer to evaluate the work-up to maximum force and maximum stress of the healed wound on day 15. Results: In the experimental groups, bromleain caused significant wound contraction and reduced granulation tissue formation by day 7 (p=0.003); increased neovasculars (new small vessels that appear in the wound area during wound healing) on days three, five and seven (p=0.001); significantly increased fibroblasts on day five but decreased by day seven (p=0.002); and significantly decreased macrophage numbers and epithelium thickness on all days of study (p=0.005). Wound strength significantly increased in experimental groups by day 15. Conclusion: Bromelain has a wide range of therapeutic benefits, but in most studies the mode of its action is not properly understood. It has been proved that bromelain has no major side effects, even after prolonged use. According to the results of this study, bromelain can be used as an effective health supplement to promote and accelerate wound healing indices, reduce inflammation and improve biomechanical parameters in diabetic wounds.

Wireless Electrical Stimulation

2013 ◽  
Vol 12 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Ourania Castana ◽  
Aekaterini Dimitrouli ◽  
Theodoros Argyrakos ◽  
Emilia Theodorakopoulou ◽  
Nektarios Stampolidis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Beneficial Effect ◽  
Lower Limb ◽  
Therapeutic Option ◽  
Healing Process ◽  
Wound Healing Process ◽  
Effective Therapeutic Option ◽  
Chronic Ulcers ◽  
Stimulation Technique

High-voltage electrical stimulation has been long proposed as a method of accelerating the wound healing process. Its beneficial effect has been successfully evaluated in the treatment of a number of chronic ulcers and burns. We present here the implementation of a new wireless electrical stimulation technique for the treatment of a complicated chronic ulcer of the lower limb. The device is transferring charges to the wound, without any contact with it, creating a microcurrent that is able to generate the current of injury. The results suggest that this easy-to-use method is an effective therapeutic option for chronic ulcers.

scholarly journals Investigation of Wound Healing Potential of Azadirachta indica Seed Extract using Wistar Rats

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
I. A. Ajayi ◽  
O. B. Omolere
Keyword(s):  
Wound Healing ◽  
Azadirachta Indica ◽  
Wistar Rats ◽  
Wound Closure ◽  
Healing Process ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Phytochemical Screening ◽  
Wound Area ◽  
Seed Extracts

This study investigated the wound healing potential of hexane and methanolic seed extracts of Azadirachta indica using 35 wistar rats that were divided into 5 groups of 7 rats each. Phytochemical screening and antimicrobial activity of the extracts were carried out while the wound healing potential was evaluated by treating the test rats with 5 % and 10 % hexane and methanol extracts in an experiment that lasted for 21 days. Wound area and percentage of wound closure of the rats were noted at four-day intervals and at 21 days, the blood and organs of the rats were subjected to haematological and histopathological analyses respectively. The extracts were found to contain tannins, glycosides and phenols and they inhibited the growth of tested organisms. All the test rats displayed better and faster healing than the control ones but there were no significant differences between their haematological and histophatological results. The seed extracts quickened the wound healing process of the rats and might therefore be useful in wound treatment.

scholarly journals Features of the Dynamics of Infected and Septic Wounds Microcirculation in Children

2016 ◽  
Vol 23 (3) ◽  
Author(s):  
O. V. Spahiu ◽  
A. P. Paholchuk
Keyword(s):  
Wound Healing ◽  
Blood Flow ◽  
Healing Process ◽  
Wound Healing Process ◽  
Doppler Flowmetry ◽  
Wound Area ◽  
Average Value ◽  
Process Dynamics ◽  
Area Monitoring ◽  
Microcirculation Parameter

Treatment of infected and septic wounds remains one of the most important problems of pediatric surgery. According to the authors, the wound process monitoring affects the treatment outcome not less than operative technique.The objective of the research was to examine the dynamics of microcirculation of the infected and septic wounds in children.Materials and methods. The analysis of the study of infected and septic wounds microcirculation in 178 children was performed. Blood flow laser analyzer (“LAKК-02” RPE “Lazma”, Russia) was used to study the blood flow in the wound area. Monitoring of wound healing process dynamics was conducted by studying the wound healing cytogram.Results. On the first day the average parameter of microcirculation was markedly increased in the area of wound edges in comparison with the contralateral area. The subsequent study of a blood flow during 5 days showed that average value of microcirculation parameter in the wound area decreased by more than 2 times in comparison with the figures on the first day. Microcirculation parameters of in the wound area further decreased.Conclusions. Dynamics of microcirculation changes in wound healing process significantly corresponds to cytogram of impression smears obtained from infected and septic wounds in children. Laser Doppler flowmetry is an effective method microcirculation control and minimally invasive diagnostic method of wound healing process.

scholarly journals Evaluation of Indonesian and two types of Japanese honey on the acceleration of cutaneous wound healing: an experimental study in mice

2019 ◽  
Vol 2 (2) ◽  
pp. 80-88
Author(s):  
Haryanto Haryanto ◽  
Kanae Mukai ◽  
Nakajima Yukari ◽  
Mayumi Okuwa ◽  
Cau Kim Jiu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Wound Area ◽  
Hydrocolloid Dressing ◽  
Thickness Skin ◽  
Microscopic Evaluation ◽  
Acacia Honey ◽  
Macroscopic Findings

The purpose of this study is to evaluate and clarify the effect of Indonesian honey, two types of Japanese honey and hydrocolloid dressing on wound healing process. Four groups of male mice were treated to produce two circular full-thickness skin wounds on the dorsum. They were then randomly allocated to receive daily Indonesian honey, Japanese honey (Buckwheat and Acacia honey) or hydrocolloid dressing as a control for treatment application. Macroscopic findings were observed from day 0 to 14 after wounding. Microscopic evaluation was assessed using qualitative analysis. The ratios of wound areas for honey groups on day 3 and 7 were smaller than those of the control group. Wound areas of honey groups gradually decreased to almost the same wound area as the control group on day 14, while the wound area of the control group peaked on day 5 and rapidly decreased until day 14. Microscopic finding that Indonesian honey was different with Japanese honey especially Buckwheat honey.

scholarly journals Wound-healing activity of immobilized postbiotics from Bacillus subtilis exometabolites

2021 ◽  
Vol 40 ◽  
pp. 01002
Author(s):  
Dina Shokatayeva ◽  
Irina Savitskaya ◽  
Aida Kistaubayeva
Keyword(s):  
Wound Healing ◽  
Bacillus Subtilis ◽  
Healing Process ◽  
Antagonistic Activity ◽  
Healing Time ◽  
Wound Dressings ◽  
Laboratory Animals ◽  
Wound Healing Process ◽  
Antimicrobial Substances ◽  
Treatment Of Wounds

The biological activity of postbiotic from Bacillus subtilis exometabolites was determined due to protein content - 0.541±13.4 mg/ml, the level of proteases - 7.8±0.3 U/ml and the presence of antimicrobial substances. A biocomposite material was developed by co-aggregation of bacterial cellulose, chitosan and Bacillus exometabolites. Modified BC gel film possesses high antagonistic activity against causative agents of wound infections: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis. The use of the obtained material in treatment of wounds on laboratory animals reduces healing time by an average of 20%. The developed bioactive wound dressings is intended for local application in order to optimize the wound healing process.

scholarly journals Efek aplikasi topikal gel ekstrak pandan wangi terhadap penyembuhan luka gingiva

2017 ◽  
Vol 2 (2) ◽  
pp. 53
Author(s):  
Icha Nofikasari ◽  
Afifah Rufaida ◽  
Chynintia Dewi Aqmarina ◽  
Failasofia Failasofia ◽  
Annisa Rahmi Fauzia ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Healing Process ◽  
Punch Biopsy ◽  
Positive Control ◽  
Negative Control ◽  
Wound Healing Process ◽  
Wound Area ◽  
Pandanus Amaryllifolius

The Effect of topical application pandan extract gel on gingival wound. Post-gingivectomy wound is usually covered by periodontal dressing, which generally contains some chemical compounds to protect the wound. However, it can provide allergic effect on some patients. Pandan leaves (Pandanus amaryllifolius Roxb.) contain a number of active substances that have anti-inflammatory, antioxidant and antibacterial effect and play a role in wound healing. This study aims to determine the effect of topical application of Pandan leaf extract gel on gingival wound healing. Gingivectomy model was carried out on mandibular incisive gingival using the 2.5-mm punch biopsy. Thirty-six rats were randomly divided into 3 groups of treatment: negative control (CMC-Na), positive control (Aloclair), and 50% of pandan extract gel. The gels on each group were applied (twice in a day) to the wound area after gingivectomy. The observation of the wound healing process was also carried out on day 1, 3, 7, and 14 by making the histological preparations of gingival wound area. The number of blood vessels was observed using microscope and data was analysed using Two- Way Anova and LSD. The result showed that number of blood vessel increased on day 3 and the peak was on day 7. Anova and LSD test showed several significant differences comparison the number blood vessel between treatment and control. In conclusion, topical application Pandan leaves extract gel could accelerate gingival wound healing.ABSTRAKLuka pasca gingivektomi dibalut dengan periodontal dressing yang mengandung senyawa kimia dengan tujuan melindungi luka, namun senyawa kimia periodontal dressing yang ada di pasaran dapat menimbulkan efek alergi terhadap beberapa pasien. Daun pandan wangi (Pandanus amaryllifolius Roxb.) mengandung zat aktif yang memiliki anti inflamasi, antioksidan, dan antibakteri kemungkinan berperan dalam proses penyembuhan luka. Penelitian ini bertujuan untuk mengetahui peran gel ekstrak daun pandan wangi dalam proses penyembuhan luka pasca gingivektomi pada tikus wistar melalui pengamatan jumlah pembuluh darah. Model gingivektomi dilakukan pada gingiva incisivus mandibula dengan menggunakan punch biopsy diameter 2,5 mm. Tiga puluh enam tikus dibagi secara acak ke dalam 3 kelompok perlakuan yaitu kontrol negatif (Gel CMC-Na), kontrol positif (Aloclair), dan gel ekstrak pandan wangi 50%. Gel uji pada masing- masing kelompok diaplikasikan pada area luka pasca gingivektomi dua kali sehari. Pengamatan proses penyembuhan luka dilakukan pada hari ke 1, 3, 7, dan 14 dengan membuat preparat histologi gingiva area luka. Parameter penyembuhan luka yang diamati adalah jumlah pembuluh darah. Data jumlah pembuluh darah dianalisis dengan menggunakan uji statistik parametrik. Hasil penelitian menunjukkan jumlah pembuluh darah mengalami peningkatan mulai hari ke-3 dan mencapai puncaknya pada hari ke-7. Hasil uji two way Anova menunjukkan adanya perbedaan yang bermakna rerata jumlah pembuluh darah antar kelompok perlakuan dengan kontrol positif maupun negatif (p<0,05). Hasil uji LSD juga menunjukkan perbedaan yang bermakna perbandingan kelompok kontrol dan perlakuan pada semua hari pengamatan. Kesimpulan penelitian ini adalah gel ekstrak pandan wangi 50% dapat mempercepat proses penyembuhan luka pasca gingivektomi dengan kemampuannya meningkatkan jumlah pembuluh darah.

scholarly journals 27. The Effectiveness Of High Protein Nutrient To The Post Sectio Caesarea Healing Process

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Darmawati Darmawati ◽  
Cut Husna ◽  
Aida Fitri ◽  
Dahrul Munira
Keyword(s):  
Wound Healing ◽  
Cesarean Section ◽  
Treatment Time ◽  
Healing Process ◽  
Intervention Group ◽  
High Protein ◽  
Sectio Caesarea ◽  
Control Group ◽  
Wound Healing Process ◽  
Significant Difference

The prevalence of cesarean section (SC) continues to increase along with the quality of technical facilities improvement. Failure to recover the surgical wounds after cesarean section is one of the possible SC complications that should be taken seriously. Proper nutrition greatly affect to wound healing. This study aims to found the effectiveness of high protein nutrients intake for post SC wound healing process at dr. Zainoel Abidin Hospital Banda Aceh. Design: This study is an experimental research with post test for intervention and control group design and using purposive sampling with the observation format as the measuring instrument. A total of 30 mother with post sectio caesarea participated in this study. There was a significant difference between post-SC wound healing in the intervention group (46.7%) compare to the control group (6.7%) (0.000). The mean of wound healing in the proliferation phase in the intervention group was faster than the control group respectively (10.47; 7.93). The hospital should provide information and evaluate high protein intake especially in post-SC patients during the treatment time, so that the patient is able to apply the information until they are going back home.

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