Population Genomic Analysis of North American Eastern Wolves (Canis lycaon) Supports Their Conservation Priority Status

The threatened eastern wolf is found predominantly in protected areas of central Ontario and has an evolutionary history obscured by interbreeding with coyotes and gray wolves, which challenges its conservation status and subsequent management. Here, we used a population genomics approach to uncover spatial patterns of variation in 281 canids in central Ontario and the Great Lakes region. This represents the first genome-wide single nucleotide polymorphism (SNP) dataset with substantial sample sizes of representative populations. Although they comprise their own genetic cluster, we found evidence of eastern wolf dispersal outside of the boundaries of protected areas, in that the frequency of eastern wolf genetic variation decreases with increasing distance from provincial parks. We detected eastern wolf alleles in admixed coyotes along the northeastern regions of Lake Huron and Lake Ontario. Our analyses confirm the unique genomic composition of eastern wolves, which are mostly restricted to small fragmented patches of protected habitat in central Ontario. We hope this work will encourage an innovative discussion regarding a plan for managed introgression, which could conserve eastern wolf genetic material in any genome regardless of their potential mosaic ancestry composition and the habitats that promote them.

Moose (Alces alces) predation by eastern coyotes (Canis latrans) and eastern coyote × eastern wolf (Canis latrans × Canis lycaon) hybrids

It has been widely assumed that coyotes (Canis latrans Say, 1823) are incapable of killing adult moose (Alces alces (L., 1758)) and previous studies of coyote predation support this assumption. However, eastern coyotes and eastern coyote × eastern wolf (Canis lycaon Schreber, 1775) are larger than western coyotes and appear to rely on larger prey in some areas. We used a combination of GPS telemetry, genetic analysis, and field investigation to test the hypothesis that eastern coyotes and coyote × wolf hybrids are capable of preying on adult moose in central Ontario. Our hypothesis was supported, as we documented four definitive cases of eastern coyotes and (or) eastern coyote × eastern wolf hybrids killing moose ≥1.5 years old. Predation by coyotes and coyote × wolf hybrids probably does not represent a threat to moose population viability in central Ontario, but our results suggest that researchers and managers in other areas with declining moose populations that are sympatric with eastern coyotes and (or) coyote × wolf hybrids should consider coyote predation as a potential source of mortality.

Genetic and morphological differentiation of wolves (Canis lupus) and coyotes (Canis latrans) in northeastern Ontario

Gray wolves ( Canis lupus L., 1758), eastern wolves ( Canis lycaon Schreber, 1775), and coyotes ( Canis latrans Say, 1823) are presently managed as a single biological population in primary wolf range in Ontario with the intent of minimizing incidental harvest of wolves. This management strategy is based on the assumption that wolves and coyotes cannot be reliably distinguished because of hybridization, and the resulting restrictions on coyote harvest are unpopular with hunters and farmers. We genetically and morphologically characterized a sample of sympatric wolves and coyotes harvested in the Lesser Clay Belt area of northeastern Ontario in 2006–2009 to test the hypothesis that these species cannot be reliably distinguished. We found that wolves and coyotes were genetically and morphologically distinct, with minimal hybridization between them. Our findings suggest that wolves and coyotes in the sampled area can be reliably distinguished, but further sampling is required to determine the full extent of areas in Ontario where wolves and coyotes are reliably distinguishable. We discuss unresolved issues regarding the feasibility of separate management for these species. We also discuss implications of our findings regarding wolf recovery in the northeastern United States.

Wolf body mass, skull morphology, and mitochondrial DNA haplotypes in the Riding Mountain National Park region of Manitoba, Canada

Two types of wolves, gray ( Canis lupus L., 1758) and eastern ( Canis lupus lycaon Schreber, 1775 or Canis lycaon ) or Great Lakes wolves, representing Old World (OW) and New World (NW) mitochondrial DNA (mtDNA) haplotypes, have been reported in eastern Canada and the Great Lakes region. Both haplotypes were found in Duck Mountain Provincial Park and Forest, Manitoba. Only OW haplotypes have been reported from the isolated Riding Mountain National Park (RMNP), 30 km to the south. Wolves with NW haplotypes hybridize with C. lupus and coyotes ( Canis latrans Say, 1823) and could mediate gene flow between canids. We examined available data on wolf body mass, skull morphology, and mtDNA from the RMNP region, as well as mtDNA from Manitoba and Saskatchewan, to assess the occurrence of NW haplotypes in wolves and possible canid hybridization. Mean body mass of female (n = 54) and male (n = 42) RMNP wolves during 1985–1987 was higher than that of females (n = 12) and males (n = 8) during 1999–2004. Thirteen skull measures from 29 wolf skulls did not suggest significant differences between RMNP and Duck Mountain wolves. Nineteen of 20 RMNP samples had OW haplotypes, whereas one clustered together with NW haplotypes.

What is the taxonomic identity of Minnesota wolves?

The taxonomic identity of the historical and current wolf ( Canis lupus L., 1758 or Canis lycaon Schreber, 1775 or their hybrids) population in Minnesota (MN) and the Great Lakes region has been, and continues to be, controversial. So too does its legal status under the U.S. Endangered Species Act. This review summarizes the morphological and genetic information about that population and concludes that historically the MN population consisted of a gray wolf (C. lupus) in the west and an eastern type ( Canis lupus lycaon or C. lycaon) in the east with intergrades or hybrids between the two in most of the state. After extirpation in much of its original MN range, the now-recovered population was infused with gray wolves from Ontario but still consists of hybrid lycaon × gray wolves, probably with higher content gray wolves in the west and higher content lycaon in the east but with most wolves morphologically appearing to be gray wolves. Because the current Wisconsin and Michigan wolf population was derived from MN wolves, they would be primarily hybrids as well. Future research should seek to relate genetic data with morphological measurements in MN wolves. In addition, attempts to breed coyotes ( Canis latrans Say, 1823) with gray wolves in captivity would shed considerable light on the controversy over the origin and taxonomic identity of the newly proposed C. lycaon.

Genetic analysis of historic western Great Lakes region wolf samples reveals early Canis lupus / lycaon hybridization

The genetic status of wolves in the western Great Lakes region has received increased attention following the decision to remove them from protection under the US Endangered Species Act. A recent study of mitochondrial DNA has suggested that the recovered wolf population is not genetically representative of the historic population. We present microsatellite genotype data on three historic samples and compare them with extant populations, and interpret published genetic data to show that the pre-recovery population was admixed over a century ago by eastern wolf ( Canis lycaon ) and grey wolf ( Canis lupus ) hybridization. The DNA profiles of the historic samples are similar to those of extant animals in the region, suggesting that the current Great Lakes wolves are representative of the historic population.

Wolf body mass cline across Minnesota related to taxonomy?

Recent genetic studies suggest that in northern Minnesota two species of wolves (Canis lupus L., 1758 or western wolf and Canis lycaon Schreber, 1775 (= Canis rufus Audubon and Bachman, 1851) or eastern wolf) meet and hybridize. However, little morphological information is available about these two types of wolves in Minnesota. We analyzed the mass of 950 female wolves and 1006 males older than 1 year from across northern Minnesota and found that it increased from 26.30 ± 0.56 kg (mean ± SE) for females and 30.60 ± 0.72 kg for males in northeastern Minnesota to 30.01 ± 0.43 kg for females and 35.94 ± 0.45 kg for males in northwestern Minnesota (females: r2 = 0.79, P < 0.02; males: r2 = 0.63, P = 0.06). These mass differences add morphological information to the identities of eastern and western wolves and support the view that ranges of the two species meet in Minnesota.

Behavioural response of eastern wolves (Canis lycaon) to disturbance at homesites and its effects on pup survival

Human disturbance at wolf dens and rendezvous sites (homesites) may have direct effects on pup survival and could result in the alteration of homesite-use characteristics. During a demographic study of eastern wolves ( Canis lycaon ) in Algonquin Provincial Park, Ontario, we entered active homesites to tag pups with VHF radio transmitters. Homesite attendance and pup survival data collected in 2003–2005 were used to determine (i) the immediate response of wolves to disturbance at homesites, (ii) whether distance moved between homesites was influenced by disturbance, (iii) if pup survival was compromised by researcher disturbance, and (iv) whether reuse in subsequent years differed between disturbed and undisturbed den sites. Packs tended to relocate pups after a disturbance, though we did not detect a difference in distances travelled between natural homesite shifts and those following disturbance. Disturbed homesites were reused in following years at a similar frequency as undisturbed homesites (6 of 15 vs. 8 of 22 homesites, respectively). Although postcapture mortality risk was slightly elevated for pups, we could not detect long-term effects of disturbance. This resilience to disturbance, and our documentation of effective techniques for radio-tagging young pups, demonstrate that research on wolf pup demography can be performed effectively without causing unacceptable negative impacts on wolf behaviour or mortality.

Reconstructing Changes in Abundance of White-tailed Deer, Odocoileus virginianus, Moose, Alces alces, and Beaver, Castor canadensis, in Algonquin Park, Ontario, 1860-2004

The history of White-tailed Deer, Odocoileus virginianus, Moose, Alces alces, and Beaver, Castor canadensis, in Algonquin Park since the 1860s is reviewed and placed in the context of changes to the forest, weather, and parasitic disease. Deer seem to have been abundant in the late 1800s and early 1900s whereas Moose were also common but less so than deer. Deer declined through the 1920s as Moose probably increased. Deer had recovered by the 1940s when Moose seem to have been scarce. The deer population declined again in the 1960s, suffered major mortality in the early 1970s, and has never recovered; deer are essentially absent from the present day Algonquin landscape in winter. Moose increased steadily following the decline of deer and have numbered around 3500 since the mid-1980s. Beaver were scarce in the Park in the late 1800s but recovered by 1910 and appear to have been abundant through the early 1900s and at high numbers through mid-century. The Beaver population has, however, declined sharply since the mid-1970s. These changes can best be explained by the history of change to the structure and composition of the Park's forests. After extensive fire and logging in the late 1800s and early 1900s, the forest is now in an essentially mature state. Weather and parasitic disease, however, have also played a role. These three species form the prey base of Algonquin's Wolves, Canis lycaon, and the net decline of prey, especially deer, has important implications for the future of wolves in the Park.