scholarly journals EFFECTS OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS ON SHEEP KIDNEYS

2021 ◽  
Vol 25 (1) ◽  
pp. 173-179
Author(s):  
Majid N. Hussain
Keyword(s):  
Cell Proliferation ◽  
Basement Membrane ◽  
Corynebacterium Pseudotuberculosis ◽  
Caseous Lymphadenitis ◽  
Electron Microscopic ◽  
Glomerular Cell

Light and electron microscopic examinations were performed on kidneys of sheep infected with caseous lymphadenitis ( CLA ) caused by Corynebacterium pseudotuberculosis Membranproliferative glomerulonephritis was demonstrated. There were irregular thickenings of the glomeruelar basement membrane and glomerular cell proliferation, in contrast, no such lesions were found in the control lambs, indicating that CLA play a role in inducing glomerulorephritis

Acute inflammation is the harbinger of glomerulosclerosis in anti-glomerular basement membrane nephritis

1995 ◽  
Vol 268 (2) ◽  
pp. F258-F265 ◽  
Author(s):  
A. L. Scandrett ◽  
J. Kissane ◽  
J. B. Lefkowith
Keyword(s):  
Cell Proliferation ◽  
Basement Membrane ◽  
Serum Creatinine ◽  
Chronic Inflammation ◽  
Glomerular Basement Membrane ◽  
Acute Inflammation ◽  
Data Set ◽  
Glomerular Cell ◽  
Immune Mediated ◽  
Acute And Chronic Inflammation

The degree of glomerular inflammation and injury during immune-mediated glomerulonephritis is felt to be critical to the eventual development of glomerulosclerosis, although the relative contributions of acute and chronic inflammation are uncertain. By grading the initial dose of antibody in accelerated anti-glomerular basement membrane nephritis, we observed that only animals with the most substantial acute inflammation (in terms of neutrophil influx and acute proteinuria) developed sustained proteinuria followed by an increase in serum creatinine and evidence of severe glomerulosclerosis. Chronic inflammation (i.e., glomerular macrophage influx and evidence of glomerular cell proliferation), in contrast, was evident without the development of glomerulosclerosis. Decreasing the degree of acute inflammation during severe nephritis by complement depletion diminished both the initial and sustained proteinuria and the influx of neutrophils, prevented the terminal increase in serum creatinine, and attenuated the evolution of glomerulosclerosis. Complement depletion, however, did not affect peak proteinuria, macrophage influx, or glomerular cell proliferation. Regression analysis of the entire data set demonstrated that acute (day 1) proteinuria was predictive of the eventual histopathological index, more so than chronic (day 7) proteinuria. To recapitulate, glomerulosclerosis following antiglomerular basement membrane nephritis appears to be substantially dependent on the degree of acute inflammatory injury.

scholarly journals Studies on the localization of the glycoprotein GP-2 within the renal glomerulus in vivo and in cultured kidney cell strains in vitro.

1981 ◽  
Vol 29 (11) ◽  
pp. 1237-1242 ◽  
Author(s):  
T D Oberley ◽  
A E Chung ◽  
J E Murphy-Ullrich ◽  
D F Mosher
Keyword(s):  
Basement Membrane ◽  
Basement Membranes ◽  
Molecular Organization ◽  
Electron Microscopic Level ◽  
Positive Staining ◽  
Electron Microscopic ◽  
Antibody Technique ◽  
Glomerular Cell ◽  
Kidney Glomerulus

Positive staining for the glycoprotein GP-2 was demonstrated in the kidney glomerulus by use of the indirect peroxidase-labeled antibody technique. At the ultrastructural level, heaviest staining for GP-2 was demonstrated along the lamina rara externa and lamina rara interna of the glomerular and tubular basement membranes, demonstrating definite molecular organization for structures which appear amorphous even at the electron microscopic level. However, GP-2 was also present in the lamina densa of the glomerular basement membrane, but not of the tubular basement membrane. The staining for GP-2 is in contrast to the predominantly mesangial staining for fibronectin. Using the indirect immunoperoxidase techniques for kidney cells cultured in vitro, it was demonstrated that cell surfaces of specific subpopulations of glomerular cells stained heavily for both fibronectin and GP-2, while renal medullary cells did not stain at all using specific antiserum to these molecules. GP-2 was present extracellularly and showed moderate staining in glomerular cell culture, while fibronectin showed heavy staining in this location.

Electron microscopic radioautographic studies on the incorporation of 3H proline in the glomerular basement membrane (GBM) in antistreptococcal-cell-membrane (SCM) injected mice

1984 ◽  
Vol 42 ◽  
pp. 188-189
Author(s):  
R.P. Nayyar ◽  
C.F. Lange ◽  
J. L. Borke
Keyword(s):  
Body Weight ◽  
Cell Membrane ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Mesangial Matrix ◽  
Electron Microscopic ◽  
Group A ◽  
Injection Protocol

Streptococcal cell membrane (SCM) antiserum injected mice show a significant thickening of glomerular basement membrane (GBM) and an increase in mesangial matrix within 4 to 24 hours of antiserum administration (1,2,3). This study was undertaken to evaluate the incorporation of 3H proline into glomerular cells and GBM under normal and anti-SCM induced conditions. Mice were administered, intraperitoneally, 0.1 ml of normal or anti-SCM serum followed by a 10 µC/g body weight injection of 3H proline. Details of the preparation of anti-SCM (Group A type 12 streptococcal pyogenes) and other sera and injection protocol have been described elsewhere (2). After 15 minutes of isotope injection a chase of cold proline was given and animal sacrificed at 20 minutes, 1,2,4,8,24 and 48 hours. One of the removed kidneys was processed for immunofluorescence, light and electron microscopic radioautographic studies; second kidney was used for GBM isolation and aminoacid analysis.

The Effect of Androgen Depletion and Replacement on the Epithelium of the Seminal Vesicle of the Aging Rat

1975 ◽  
Vol 33 ◽  
pp. 590-591
Author(s):  
Venita F. Allison
Keyword(s):  
Cell Proliferation ◽  
Seminal Vesicle ◽  
Male Rats ◽  
Target Cells ◽  
Cell Regeneration ◽  
Secretory Function ◽  
Electron Microscopic ◽  
Aging Rat ◽  
Microscopic Studies ◽  
Androgen Depletion

In 1930, Moore, Hughes and Gallager reported that after castration seminal vesicle epithelial cell atrophy occurred and that cell regeneration could be achieved with daily injections of testis extract. Electron microscopic studies have confirmed those observations and have shown that testosterone injections restore the epithelium of the seminal vesicle in adult castrated male rats. Studies concerned with the metabolism of androgens point out that dihydrotestosterone stimulates cell proliferation and that other metabolites of testosterone probably influence secretory function in certain target cells.Although the influence of androgens on adult seminal vesicle epithelial cytology is well documented, little is known of the effect of androgen depletion and replacement on those cells in aging animals. The present study is concerned with the effect of castration and testosterone injection on the epithelium of the seminal vesicle of aging rats.

The blister of porphyria cutanea tarda: A Scanning and Transmission Electron Microscopic study

1986 ◽  
Vol 44 ◽  
pp. 334-335
Author(s):  
Veronika Burmeister ◽  
R. Swaminathan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Basement Membrane ◽  
Middle Age ◽  
Porphyria Cutanea Tarda ◽  
Minor Trauma ◽  
Electron Microscopic ◽  
Transmission Electron Microscopic Study ◽  
Transmission Electron ◽  
Transmission Electron Microscopic

Porphyria cutanea tarda (PCT) is a disorder of porphyrin metabolism which occurs most often during middle age. The disease is characterized by excessive production of uroporphyrin which causes photosensitivity and skin eruptions on hands and arms, due to minor trauma and exposure to sunlight. The pathology of the blister is well known, being subepidermal with epidermodermal separation, it is not always absolutely clear, whether the basal lamina is attached to the epidermis or the dermis. The purpose of our investigation was to study the attachment of the basement membrane in the blister by comparing scanning with transmission electron microscopy.

scholarly journals POLYMORPHONUCLEAR LEUKOCYTES IN IMMUNOLOGIC REACTIONS

1966 ◽  
Vol 124 (4) ◽  
pp. 733-752 ◽  
Author(s):  
Charles G. Cochrane ◽  
Barbara S. Aikin
Keyword(s):  
Basement Membrane ◽  
Polymorphonuclear Leukocytes ◽  
Electron Microscopic Observation ◽  
Severe Damage ◽  
Electron Microscopic ◽  
Cationic Proteins ◽  
Vascular Basement Membrane ◽  
Arthus Phenomenon ◽  
Immunologic Reactions

Vascular basement membrane was disrupted in the presence of polymorphonuclear leukocytes (PMN's) during two immunologic reactions: The Arthus phenomenon and the reaction to locally injected antibody to vascular basement membrane. This disruption was evidenced by (a) the inability of the basement membrane to retain circulating carbon, by (b) loss of antigenic constituents, and by (c) electron microscopic observation showing actual gaps in the structure of the vascular basement membrane. The factors within PMN's responsible for damage to isolated glomerular basement membrane in vitro were found by isolation procedures to be cathepsins D and E. Cationic proteins of PMN's were separable from the cathepsins. While inducing vascular permeability upon injection, these basic proteins failed to inflict the severe damage to the basement membrane observed in Arthus and antibasement membrane reactions. It is concluded that the full expression of these immunologic lesions requires destruction of the basement membrane possibly brought about by cathepsins D and E. Some of the physicochemical properties of these pathologically active leukocytic factors are given.

scholarly journals Resistance of Corynebacterium pseudotuberculosis in the Brazilian semiarid environment

2018 ◽  
Vol 38 (6) ◽  
pp. 1091-1096 ◽  
Author(s):  
Maria C.A. Sá ◽  
Samily A.S. Oliveira ◽  
Edmilson M. Dantas Jr ◽  
Gisele V. Gouveia ◽  
João J.S. Gouveia ◽  
...  
Keyword(s):  
Survival Rates ◽  
Control Measures ◽  
Corynebacterium Pseudotuberculosis ◽  
Maximum Temperature ◽  
Caseous Lymphadenitis ◽  
Biofilm Production ◽  
Brazilian Semiarid ◽  
Higher Temperature ◽  
Inoculum Survival

ABSTRACT: The semiarid northeast of Brazil contains a unique biome known as caatinga, with a maximum temperature of 40 ºC and a relativity humidity of 56%. The caatinga is characterized by a variety of plants, including Cereus jamacaru Dc (mandacaru), Poincianella microphylla Mart. ex G. Don (catingueira), Pilosocereus gounellei FAC Weber (xique-xique) and Mimosa tenuiflora (Willd.) Poir (jurema preta). Sheep and goat industries are economically strong in that region, despite the fact that caseous lymphadenitis is highly prevalent. The aim of the present study was to assess the survival and biofilm production of Corynebacterium pseudotuberculosis isolates in the environment and under controlled temperatures (28°C, 37°C and 42°C) under different surfaces (plants, soil, wood, wire and thorns). In addition, we investigated the effects of applying the disinfectants chlorhexidine, hypochlorite and quaternary ammonia in soil, tiles, wood and vegetation cover. Four strains of C. pseudotuberculosis were selected (two from goats and two from sheep) for inoculation according to their in vitro biofilm production. Adherence to microplates was used to assess the biofilm-forming ability of the bacteria. Lower survival rates were observed when isolates of C. pseudotuberculosis were subjected to a temperature of 42°C. In terms of caatinga biome plants, contamination of jurema-preta plants resulted in the lowest survival rates. The disinfectant quaternary ammonia promoted a lower inoculum survival in all surfaces. The disinfectants and the higher temperature contributed to the reduction of biofilm production in isolates of C. pseudotuberculosis. knowledge of these patterns is important for the establishment of disease control measures, given the questionable efficacy of the treatment and the immuno-prophylaxis of caseous lymphadenitis.

Antimicrobial susceptibility of Corynebacterium pseudotuberculosis isolated from lesions of caseous lymphadenitis in sheep in Hokkaido, Japan.

1991 ◽  
Vol 53 (2) ◽  
pp. 355-356 ◽  
Author(s):  
Hong-Kun ZHAO ◽  
Hiroyuki MORIMURA ◽  
Takashi HIRAMUNE ◽  
Naoya KIKUCHI ◽  
Ryo YANAGAWA ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Corynebacterium Pseudotuberculosis ◽  
Caseous Lymphadenitis

Analysis of elongating morphogenesis of quail anterior submaxillary gland: absence of localized cell proliferation

Development ◽  
1981 ◽  
Vol 62 (1) ◽  
pp. 229-239
Author(s):  
Hiroyuki Nogawa
Keyword(s):  
Cell Proliferation ◽  
Basement Membrane ◽  
Regional Differences ◽  
Submaxillary Gland ◽  
Mesenchymal Cells ◽  
Submaxillary Glands ◽  
Parallel Direction ◽  
Recombination Experiments

Quail anterior submaxillary glands elongated extensively without branching (more than sevenfold) from 8 to 10 incubation days. Investigation of mitotic activity of the rudiments in vivo showed no localized cell proliferation throughout the rudiments, and recombination experiments in vitro to examine regional differences in mitogenic activity of the surrounding mesenchyme also showed that no mesenchymal region specifically stimulates the epithelial cell proliferation. Histological observation of the rudiments showed that epithelial cells did not lengthen in a parallel direction to the long axis of the rudiment, and that mesenchymal cells encircled the epithelial cord perpendicularly to its axis. The basement membrane was obscure in the distal end of the rudiments, while it was easily detected in the other part of the rudiments. These results suggest that the elongating morphogenesis of the anterior submaxillary rudiments is not achieved by localized cell proliferation but by almost uniformly distributed cell proliferation, and mesenchymal cells surrounding the rudiment or the basement membrane may be involved in the controlling mechanisms of the elongating morphogenesis.

scholarly journals "Intermediate" Cell Types and Mixed Cell Proliferation in Multiple Myeloma: Electron Microscopic Observations

Blood ◽  
1966 ◽  
Vol 27 (2) ◽  
pp. 212-226 ◽  
Author(s):  
JORGE E. MALDONADO ◽  
ROBERT A. KYLE ◽  
ARNOLD L. BROWN ◽  
EDWIN D. BAYRD
Keyword(s):  
Multiple Myeloma ◽  
Cell Proliferation ◽  
Plasma Cells ◽  
Cell Transformation ◽  
Cell Types ◽  
Lymphoid Cells ◽  
Intermediate Cell ◽  
Electron Microscopic ◽  
Mixed Cell ◽  
Reticulum Cells

Abstract Bone marrow studies of multiple myeloma revealed, in some cases, a conspicuous proliferation of "lymphoid" cells, virtually indistinguishable by light microscopy from those seen in lympho-proliferative disorders. Electron microscopy demonstrated a variety of cells ranging from typical lymphocytes to cells with plasmocytoid features. Between these two types of elements there were cells with intermediate characteristics. In addition, in several cases of myeloma the presence of fixed reticuloendothelial cells and "reticular" plasma cells (or reticulum cells with plasmocytic features) was frequently noted. The presence of reticulum cells and lymphocytes and of cells apparently "intermediate" between these cellular elements and plasma cells, as judged from electron microscopic observations, is suggestive morphologic evidence of a phenomenon of cell transformation and evidence of a mixed cell proliferation in certain cases of multiple myeloma.

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