scholarly journals Antibacterial activity of Brazilian Northeast plants against Corynebacterium pseudotuberculosis

2021 ◽  
Vol 10 (11) ◽  
pp. e509101119875
Author(s):  
Maria Emília Alcântara ◽  
Antonio Pedro Fróes de Farias ◽  
Maria da Conceição Aquino de Sá ◽  
Soraya Castro Trindade ◽  
Roberto Meyer ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Small Ruminants ◽  
Inhibition Zone ◽  
Corynebacterium Pseudotuberculosis ◽  
High Prevalence ◽  
In Vitro Antibacterial Activity ◽  
Agar Well Diffusion Assay ◽  
Brazilian Northeast ◽  
Diffusion Assay

The production of small ruminants is an important economic activity of the brazilian Northeast, but some diseases have a high prevalence in this region, such as caseous lymphadenitis (CL), caused by Corynebacterium pseudotuberculosis. The treatment of CL is often ineffective, which justifies the search of new active principles from plants, mainly of the region, to have an accessible treatment. For this reason, the present study was carried out to evaluate the in vitro antibacterial activity of Annona squamosa, Azadirachta indica, Allium sativum, Prosopis juliflora and Portulaca oleracea against C. pseudotuberculosis. Agar well diffusion assay (AWD) and broth microdilution - to determine minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) - evaluated the antimicrobial activity. The highest antibacterial potential was obtained by ethanolic extracts of A. indica leaf (MIC 0,12 mg/mL for 2 strains), A. squamosa stalk (MIC 0,55 mg/mL for 3 strains) and shell (MIC 0,6 mg/mL for 3 strains). These extracts also presented the highest inhibition zone in AWD (30 mm, 38 mm and 32 mm, respectively). A. squamosa and A. indica have high antimicrobial potential against C. pseudotuberculosis.

scholarly journals In Vitro Comparative Analysis of Antibacterial Activity of Different Fractions of Corchorus capsularis and Corchorus olitorius Leaves Extracts

2020 ◽  
Vol 36 (2) ◽  
pp. 69-73
Author(s):  
Riad Raihan Abir ◽  
Mafruha Marjia ◽  
Nadira Naznin Rakhi ◽  
Otun Saha ◽  
M Anwar Hossain ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Corchorus Olitorius ◽  
E Coli ◽  
Corchorus Capsularis ◽  
Bioactive Component ◽  
High Prevalence ◽  
Agar Well Diffusion Assay ◽  
New Generation

High prevalence of antibiotic resistance is necessitating the investigation of novel antimicrobials from natural herbs and plant. So, this present study investigated two of indigenously cultivated jute plants, Corchorus capsularis (white jute)and Corchorus olitorius (tossa jute) for their antibacterial activity. Lipophilic extracts of leaves were prepared and fractionated by column chromatography resulting in 6 fractions of the extract of C. olitorius leaves (At, Bt, Ct, Dt, Et and Ft) and 11 fractions in case of white jute leaves (Aw, Bw, Cw, Dw, Ew, Fw, Gw, Hw, Iw, Jw and Kw). Each fraction of both of the leaves extracts were used to tested by agar well diffusion assay against Staphylococcus aureus and Escherichia coli along with a control organism, E. coli DH5±. While the fractions of C. olitorius leaves showed higher antibacterial activity against S. aureus, fractions of the extract of C. capsularis leaves were more effective against E. coli. The At fraction of C. olitorius extract showed the highest inhibition zone of 19 ± 2.80 mm against S. aureus and Dw fraction of C. capsularis extract had the highest inhibition zone of 15 ± 2.3 mm against E. coli (p <0.05). The extract of C. olitorius leaves showed comparatively higher antibacterial effect than that of C. capsularis leaves. Considering the promising finding regarding the antibacterial effectiveness, these fractions of the leave extract should be analyzed further to isolate the exact bioactive component to develop the lead component of new generation antibacterial drugs. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 69-73

scholarly journals In vitro SCREENING ANTIBACTERIAL ACTIVITY OF Bidens pilosa LINNÉ AND Annona crassiflora MART. AGAINST OXACILLIN RESISTANT Staphylococcus aureus (ORSA) FROM THE AERIAL ENVIRONMENT AT THE DENTAL CLINIC

2014 ◽  
Vol 56 (4) ◽  
pp. 333-340 ◽  
Author(s):  
Jeferson Junior da Silva ◽  
Cláudio Daniel Cerdeira ◽  
Juliana Moscardini Chavasco ◽  
Ana Beatriz Pugina Cintra ◽  
Carla Brigagão Pacheco da Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Toxicity Testing ◽  
Inhibition Zone ◽  
Bidens Pilosa ◽  
Total Absence ◽  
Mtt Method ◽  
In Vitro Antibacterial Activity ◽  
Annona Crassiflora

Currently multiresistant Staphylococcus aureus is one common cause of infections with high rates of morbidity and mortality worldwide, which directs scientific endeavors in search for novel antimicrobials. In this study, nine extracts from Bidens pilosa (root, stem, flower and leaves) and Annona crassiflora (rind fruit, stem, leaves, seed and pulp) were obtained with ethanol: water (7:3, v/v) and their in vitro antibacterial activity evaluated through both the agar diffusion and broth microdilution methods against 60 Oxacillin Resistant S. aureus (ORSA) strains and against S. aureus ATCC6538. The extracts from B. pilosa and A. crassiflora inhibited the growth of the ORSA isolates in both methods. Leaves of B. pilosa presented mean of the inhibition zone diameters significantly higher than chlorexidine 0.12% against ORSA, and the extracts were more active against S. aureus ATCC (p < 0.05). Parallel, toxicity testing by using MTT method and phytochemical screening were assessed, and three extracts (B. pilosa, root and leaf, and A. crassiflora, seed) did not evidence toxicity. On the other hand, the cytotoxic concentrations (CC50 and CC90) for other extracts ranged from 2.06 to 10.77 mg/mL. The presence of variable alkaloids, flavonoids, tannins and saponins was observed, even though there was a total absence of anthraquinones. Thus, the extracts from the leaves of B. pilosa revealed good anti-ORSA activity and did not exhibit toxicity.

scholarly journals Estimation of Alkaloids and Antibacterial Activity of Aconitum spicatum Bruhl Stapf from Manaslu Conservation Area

2013 ◽  
Vol 13 (1) ◽  
pp. 67-71
Author(s):  
Sajan L Shyaula ◽  
Ash B Ngakushi ◽  
Bijaya L Maharjan ◽  
Mangala D Manandhar
Keyword(s):  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Conservation Area ◽  
National Priority ◽  
Pathogenic Microbes ◽  
The Government ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

Aconitum spicatum is one of the deadly poisonous and highly valued medicinal plant. It is a national prioritized herb among 30 national priority herbs listed by the government of Nepal for their development, research and cultivation. The acute toxicity of the extract of this plant appeared to be directly related to the alkaloid content. The amount of total alkaloid of A. spicatum collected from Manaslu conservation area was calculated to be 1.7% by spectrophotometric method based on Dragendorff’s reagent. In-vitro evaluation of the crude extract of plant rhizomes using agar well diffusion assay against eight bacteria, Staphylococcus aureus, Escherichia coli, Schigella flexneri, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Salmonella typhimurium and Klebsiella pneumonia, displayed potential antibacterial activity. The diethyl ether fraction was the most effective against all pathogenic microbes with minimum bacterial concentration value 3.125-6.25 mg/ml. Nepal Journal of Science and Technology Vol. 13, No. 1 (2012) 67-71 DOI: http://dx.doi.org/10.3126/njst.v13i1.7443

scholarly journals Potential Antibacterial Activity of Bergenia purpurascens

2012 ◽  
Vol 12 ◽  
pp. 157-162 ◽  
Author(s):  
Gan B Bajracharya ◽  
Rokesh Maharjan ◽  
Bijaya L Maharjan
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Salmonella Typhi ◽  
Traditional Use ◽  
Conservation Area ◽  
Healing Of Wounds ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
And Staphylococcus Aureus

Bergenia purpurascens, an important traditional medicinal plant used as Ayurveda, was collected at 3800 m from the Manaslu Conservation Area. In vitro evaluation of the crude extracts of the plant rhizomes using agar well diffusion assay against eight bacteria displayed potential antibacterial activity. The minimum inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella paratyphi, Salmonella typhi, Salmonella typhimurium, Shiegella flexneri and Staphylococcus aureus were determined. This study supports the traditional use of the plant material for the healing of wounds and antibiotic effect.DOI: http://dx.doi.org/10.3126/njst.v12i0.6494 Nepal Journal of Science and Technology 12 (2011) 157-162 

scholarly journals In vitro antibacterial activity of some systemic and topical antihistaminic preparations

2009 ◽  
Vol 32 (6) ◽  
pp. 232
Author(s):  
Julide Sedef Gocmen ◽  
Unase Buyukkocak ◽  
Osman Caglayan
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Blood Levels ◽  
Minimal Bactericidal Concentration ◽  
High Concentration ◽  
Diphenhydramine Hydrochloride ◽  
In Vitro Antibacterial Activity ◽  
Pheniramine Maleate

Purpose: In vitro antibacterial activity of topical and systemic antihistaminic preparations containing different active substrates against the standard strains of two bacteria was evaluated. Methods: Four topical and 3 systemic preparations containing pheniramine maleate, chlorophenoxamine hydrochloride, and diphenhydramine hydrochloride were studied. The antibacterial activities of these preparations against strains of S. aureus (American Type Culture Collection, ATCC 29213) and S. epidermidis (ATCC 25212) were tested using the disc diffusion method. In addition, the Minimal Innhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of parenteral preparations for these two bacteria were determined. Results: Pheniramine maleate-topical and pheniramine maleate-systemic had no activity against bacteria, but the others showed various rates of activity. Chlorophenoxamine hydrochloride-topical and chlorophenoxamine hydrochloride-systemic were the most effective (P < 0.05). Despite the same active substrate content, diphenhydramine hydrochloride-topical-1 and diphenhydramine hydrochloride-topical-2 yielded different results when they were compared with each other or with the other preparations. Diphenhydramine hydrochloride-topical-2 had a relatively higher rate of activity than diphenhydramine hydrochloride-topical-1. Inhibition zone diameters were 16.9±1.5 mm 12.3±0.5 mm for S .aureus, 17.4±1.0 mm 0 mm for S .epidermidis respectively (P < 0.05). MIC values of parenteral preparations were equal to or above 125 ?g/ml. Conclusion:, MIC values of parenteral preparations were higher than their blood levels in clinical use. Thus, effects of parenteral preparations may not have been reflected in routine clinical practice. However, topical forms have antibacterial activity due to additive substrates and the use of high concentration levels at the site of application. Therefore, in selection of topical forms for appropriate cases, these effects should also be taken into consideration. The antibacterial activity of topical antihistaminic preparations may be useful in certain dermatological pathology.

scholarly journals In Vitro Antibacterial Activity of Black Soldier Fly (Hermetia Illucens) Larva Extracts Against Gram-Negative Bacteria

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Harlystiarini Harlystiarini ◽  
Rita Mutia ◽  
I Wayan Teguh Wibawan ◽  
Dewi Apri Astuti
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Test Method ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Black Soldier Fly ◽  
E Coli ◽  
In Vitro Antibacterial Activity ◽  
Zone Growth

The aim of this study was to evaluate the in vitro antibacterial activity of Black soldier fly (BSF) larva extract. The BSF larva was extracted using methanol and then tested for antibacterial activity using agar diffusion method (zone growth inhibition). The antibacterial activity was conducted against Salmonella sp. and Escherichia coli, two important bacterial strains in poultry, using six dilution levels (10 mg/ml, 20 mg/ml, 40 mg/ml, 80 mg/ml, 160 mg/ml and 320 mg/ml). All the results were subjected analyze using t-test method. Based on the diameter of the inhibition zone, the BSF larva extract has a strong (P<0.05) antibacterial activity against Salmonella sp. and E. coli when the concentration used 320 mg/ml. In addition, BSF larva extract also contain high amount of lauric acid (49.18%), a saturated fatty acid that has been proven to proposes as antibacterial agent. Therefore, it could be concluded that the BSF larva extract could be used as a candidate for antibacterial substances. 

scholarly journals In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)

2020 ◽  
Vol 9 (2) ◽  
pp. 128-135
Author(s):  
Muhammad Evy Prastiyanto ◽  
Inas Hasna Azizah ◽  
Hafizha Dara Haqi ◽  
Bagus Dwi Yulianto ◽  
Aulia Bella Agmala ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Seed Extract ◽  
Methicillin Resistant ◽  
Biological Source ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Seed Extracts

Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.

scholarly journals Synthesis, spectral characterization, DFT and in vitro antibacterial activity of Zn(II), Cd(II) and Hg(II) complexes derived from a new thiosemicarbazide

2019 ◽  
Vol 8 (4) ◽  
pp. 715-722 ◽  
Keyword(s):  
Thermal Stability ◽  
Antibacterial Activity ◽  
Dft Method ◽  
Inhibition Zone ◽  
Neutral Form ◽  
Inhibition Zone Diameter ◽  
Octahedral Environment ◽  
In Vitro Antibacterial Activity ◽  
Homo Lumo

A new series of mono-nuclear complexes, Cd2+, Zn2+ and Hg2+ derived from 4,4-dimethyl-2,6-dioxo-N-phenylcyclohexanecarbothioamide (HDDPT) have been prepared and characterized. The complexes adopted the molecular formulae; [Hg(DDPT)Cl(H2O)], [Cd (HDDPT) 2Cl2] and [Zn (HDDPT)2Cl2], respectively. IR data showed that HDDPT ligand acts as OS bidentate either in neutral form as in Cd2+, Zn2+ complexes or mononegative chelate in Hg2+complex. An octahedral environment was suggested for Zn2+ and Cd2+ complexes and a tetrahedral arrangement for Hg2+ complex. DFT method we used to confirm the geometries of title compounds and evaluate other energetic parameters such as HOMO, LUMO, hardness, softness and electronegativity. Also, the thermal stability was examined by TGA and DrTGA and the associated thermodynamic parameters of activation were determined using Coats-Redfern and Horowitz-Metzger methods. Also, all compounds were screened for antibacterial activity using ciprofloxacin and gentamicin drugs by inhibition zone diameter method technique.

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