Modeling surface roughness of point robot laser hardening, with emphasis on the surface

The topic of Machine Learning is so popular that it is not only the future trend, but also the money tide. Machine learning technique and intelligent system methods are very popular in mechanical engineering. Robot laser surface hardening is one of the most promising techniques for surface modification of the microstructure of a material to improve wear and corrosion resistance. For predicting the surface roughness of the hardened specimens, the support vector machine and multiple regression is used. The aim of this paper is to present modeling roughness of point robot laser hardened specimens with different parameters of robot laser cell.

Digital microfluidic isolation of single cells for -Omics

We introduce Digital microfluidic Isolation of Single Cells for -Omics (DISCO), a platform that allows users to select particular cells of interest from a limited initial sample size and connects single-cell sequencing data to their immunofluorescence-based phenotypes. Specifically, DISCO combines digital microfluidics, laser cell lysis, and artificial intelligence-driven image processing to collect the contents of single cells from heterogeneous populations, followed by analysis of single-cell genomes and transcriptomes by next-generation sequencing, and proteomes by nanoflow liquid chromatography and tandem mass spectrometry. The results described herein confirm the utility of DISCO for sequencing at levels that are equivalent to or enhanced relative to the state of the art, capable of identifying features at the level of single nucleotide variations. The unique levels of selectivity, context, and accountability of DISCO suggest potential utility for deep analysis of any rare cell population with contextual dependencies.

A Polymer Film Dye Laser with Spatially Modulated Emission Controlled by Transversely Distributed Pumping

Spatial modulation of laser emission controlled by the structure of excitation light field was demonstrated. A dye doped polymer film as an active medium was sandwiched between two laser mirrors forming a laser cell. The pumping was performed by an interference pattern formed with two mutually coherent beams of the second harmonic of a Q-switched Nd:YAG laser (532 nm) and located in the plane of the laser cell. The laser emission was observed normally on the plane of the cell. The cross section of the obtained laser emission was modulated in intensity with an interval between maximums depending on the period of the pumping interference pattern. Thus, the emitted light field qualitatively looks like diffraction from an elementary dynamic hologram, that is, a holographic diffraction grating.