Laser cell-micropatterned pair of cardiomyocytes: the relationship between basement membrane development and gap junction maturation

2014 ◽  
Vol 6 (4) ◽  
pp. 045003 ◽  
Author(s):  
Huaxiao Yang ◽  
Thomas K Borg ◽  
Lucas P Schmidt ◽  
Bruce Z Gao
Keyword(s):  
Basement Membrane ◽  
Gap Junction ◽  
Membrane Development ◽  
The Relationship ◽  
Laser Cell

scholarly journals Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation

2019 ◽  
Author(s):  
Fabiana Cerqueira Campos ◽  
Cynthia Dennis ◽  
Hervé Alégot ◽  
Cornelia Fritsch ◽  
Adam Isabella ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Ovarian Follicle ◽  
Follicle Cell ◽  
Autonomous Control ◽  
Stress Fibers ◽  
Follicle Growth ◽  
Growth Promoting ◽  
Fibers Orientation ◽  
Open Question ◽  
The Relationship

SummaryHow extracellular matrix participates to tissue morphogenesis is still an open question. In the Drosophila ovarian follicle, it has been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils and stress fibers and their respective impact on elongation are unclear. We found that Dystroglycan (Dg) and Dystrophin (Dys) are involved in BM fibril deposition. Moreover, they orient stress fibers, by acting locally and in parallel to Fat2. Nonetheless, Dg-Dys complex-mediated cell autonomous control of F-actin fibers orientation relies on the previous BM fibril deposition, indicating two distinct but interdependent functions. Thus, the Dg-Dys complex works as a critical organizer of the epithelial basal domain, regulating both F-actin and BM. Furthermore, BM fibrils act as a persistent cue for the orientation of stress fibers that are the main effector of elongation.

scholarly journals Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes

2015 ◽  
Vol 330 (1) ◽  
pp. 222-232 ◽  
Author(s):  
Huaxiao Yang ◽  
Thomas K. Borg ◽  
Honghai Liu ◽  
Bruce Z. Gao
Keyword(s):  
Basement Membrane ◽  
Interactive Relationship ◽  
Membrane Development

RELATIONSHIP BETWEEN VESSEL WALL 13-HODE PRODUCTION AND SUBENDOTHELIAL BASEMENT MEMBRANE THROMBORESISTANCE: INFLUENCE OF SALICYLATE

1987 ◽  
Author(s):  
E Weber ◽  
T A Haas ◽  
J Hirsh ◽  
M R Buchanan
Keyword(s):  
Endothelial Cells ◽  
Linoleic Acid ◽  
Basement Membrane ◽  
Vessel Wall ◽  
Carotid Arteries ◽  
Platelet Adhesion ◽  
Fold Increase ◽  
Lipoxygenase Pathway ◽  
The Relationship ◽  
Camp Levels

In previous studies, we have reported that i) the basement membrane (BM) underlying endothelial cells was initially throm-boresistant; ii) 13-hydroxyoctadecadienoic acid (13-HODE) synthesized by endothelial cells from linoleic acid via the lipoxygenase pathway, contributed to the thromboresistance of the endothelium; and iii) salicylate (SAL) increased injured vessel wall thrombogenecity. Therefore, we performed studies to determine the relationship between injured vessel wall thrombogenecity, vessel wall 13-HODE and cAMP levels, and salicylate treatment. Injured vessel wall thrombogenecity was measured as the number of H-adenine platelet (3H-PLT) adherent to the subendothelial BM exposed by air injury in carotid arteries of rabbits treated with 0 or 100 mg/kg of SAL bid, given orally2. Vessel wall 13-HODE was measured as the amount of 13-HODE/cm produced by the vessel wall following stimulation with 10μ/M linoleic acid, and measured by HPLC. Vessel wall cAMP levels were measured by RIA. Four hours after air injury, there was 25.4 ± 2 3 2H-PLT/cm2 of exposed BM. This was associated with 15.9 ng/cm2 of 13-HODE and 308 pM/cm2 of cAMP (Table 1). In contrast, in rabbits treated with SAL, there was a 2-fold increase in platelet adhesion onto the injured carotid arteries. The increase in platelet adhesion was associated with a 65% decrease in 13-HODE production by the vessel wall and a modest (20%) decrease in cAMP level.We conclude that the lipoxygenase derived linoleic acid metabolite, 13-HODE contributes not only to the thromboresis-tance of the endothelium, but also to its underlying basement membrane.

scholarly journals Interstitial volume modulates the conduction velocity-gap junction relationship

2012 ◽  
Vol 302 (1) ◽  
pp. H278-H286 ◽  
Author(s):  
Rengasayee Veeraraghavan ◽  
Mohamed E. Salama ◽  
Steven Poelzing
Keyword(s):  
Guinea Pig ◽  
Gap Junction ◽  
Conduction Velocity ◽  
Important Determinant ◽  
Cardiac Conduction ◽  
Cell Width ◽  
Interstitial Volume ◽  
Ventricular Tachycardias ◽  
The Relationship ◽  
Isolated Cell

Cardiac conduction through gap junctions is an important determinant of arrhythmia susceptibility. Yet, the relationship between degrees of Gj uncoupling and conduction velocity (θ) remains controversial. Conflicting results in similar experiments are normally attributed to experimental differences. We hypothesized that interstitial volume modulates conduction velocity and its dependence on Gj. Interstitial volume (VIS) was quantified histologically from guinea pig right ventricle. Optical mapping was used to quantify conduction velocity and anisotropy (ARθ). Albumin (4 g/l) decreased histologically assessed VIS, increased transverse θ by 71 ± 10%, and lowered ARθ. Furthermore, albumin did not change isolated cell size. Conversely, mannitol increased VIS, decreased transverse θ by 24 ± 4%, and increased ARθ. Mannitol also decreased cell width by 12%. Furthermore, mannitol was associated with spontaneous ventricular tachycardias in three of eight animals relative to zero of 15 during control. The θ-Gj relationship was assessed using the Gj uncoupler carbenoxolone (CBX). Whereas 13 μM CBX did not significantly affect θ during control, it slowed transverse θ by 38 ± 9% during mannitol (edema). These data suggest changes in VIS modulate θ, ARθ, and the θ-Gj relationship and thereby alter arrhythmia susceptibility. Therefore, VIS may underlie arrhythmia susceptibility, particularly in diseases associated with gap junction remodeling.

The Relationship of Reticular Basement Membrane Thickness to Airway Wall Remodeling in Asthma

2002 ◽  
Vol 166 (12) ◽  
pp. 1590-1595 ◽  
Author(s):  
Alan L. James ◽  
Peta S. Maxwell ◽  
Gladys Pearce-Pinto ◽  
John G. Elliot ◽  
Neil G. Carroll
Keyword(s):  
Basement Membrane ◽  
Membrane Thickness ◽  
Airway Wall ◽  
Basement Membrane Thickness ◽  
Relationship Of ◽  
The Relationship

Gap junction associated filaments in testis interstitial cells of the rat

1978 ◽  
Vol 36 (2) ◽  
pp. 550-551
Author(s):  
J.F. David-Ferreira ◽  
K.L. David-Ferreira ◽  
M.H. Miranda ◽  
J.C. Lemos
Keyword(s):  
Gap Junction ◽  
Freeze Fracture ◽  
Interstitial Cells ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Thin Sections ◽  
Cacodylate Buffer ◽  
Old Rats ◽  
The Relationship ◽  
Made In

The testis interstitial cells of the rat are closely associated forming small aggregates in the vicinity of the vessels. The finality of the present study is to analise the relationship between the interstitial cells and to describe some particularities of their junctions.The observations were made in thin sections from testis of 2 to 3 months old rats fixed by immersion or perfusion with 2 to 3% glutaral- dehyde in s-collidine or cacodylate buffer followed by 2% osmium tetro-xyde in the same buffers. Some specimens have been prepared following the technique of Shea. The thin sections obtained after embedding in Epon were double stained in uranyl acetate and lead citrate. For the cryofracture study the tissues were fixed in 3% glutaraldehyde in cacodylate buffer, impregnated in 28% glycerol, frozen in Freon 22 and stored in liquid nitrogen. Freeze fracture and platinum-carbon shadowing were done in a Balzers 300.

Ultrastructure of microfilarial sheath and its comparison with egg shell

1990 ◽  
Vol 48 (3) ◽  
pp. 494-495
Author(s):  
Lye Howe ◽  
Josephine Chun ◽  
Viqar Zaman
Keyword(s):  
Basement Membrane ◽  
Cross Section ◽  
Light Microscope ◽  
Brugia Malayi ◽  
Embryonic Cells ◽  
Stages Of Development ◽  
Broad Base ◽  
Egg Shell ◽  
Dense Projections ◽  
The Relationship

In this study the ultrastructure of the uterus of Brugia malayi and its contents were studied especially to reveal the relationship between the sheath of the microfilaria and the egg shell, as it is claimed that the egg shell ultimately forms the microfilairal sheath (Rogers et al, 1976).The cross section of the worm revealed a distinct cuticle, musculature, a single patent fut and paired uteri. The uterine contents were filled with eggs at various stages of development. Inside each egg were embryonic cells which corresponded to the so-called “nuclei” of microfilaria which are seen in the light microscope. The egg shell was discernable as a membrane with minute electron-dense projections on its surface. At higher magnification the egg shell was seen to consist of two structures - a homogenous basement membrane and electron dense projections which rested on it. These projections were found all around the egg shell and consisted of a broad base and a pointed anterior end.

scholarly journals Major loss of the 28-kD protein of gap junction in proliferating hepatocytes.

1987 ◽  
Vol 105 (4) ◽  
pp. 1925-1934 ◽  
Author(s):  
R Dermietzel ◽  
S B Yancey ◽  
O Traub ◽  
K Willecke ◽  
J P Revel
Keyword(s):  
Monoclonal Antibody ◽  
Gap Junction ◽  
The Other ◽  
Regenerating Liver ◽  
Cultured Hepatocytes ◽  
Junction Protein ◽  
Major Loss ◽  
Gap Junction Protein ◽  
Quantitative Changes ◽  
The Relationship

There is a reduction in the 28-kD gap junction protein detectable by immunofluorescence in livers of partially hepatectomized rats and in cultured hepatocytes stimulated to proliferate. By the coordinate use of antibodies directed to the hepatic junction protein (HJP28) and the use of a monoclonal antibody that recognizes bromodeoxyuridine (BrdU) incorporated into DNA, we have been able to study the relationship between detectable gap junction protein and cell division. Hepatocytes that label with BrdU in the regenerating liver and in cell culture show a significant reduction of HJP28. Cells that do not synthesize DNA, on the other hand, show normal levels and distribution of immunoreactive gap junction protein. We postulate that the quantitative changes in gap junction expression might play an important role in the control of proliferation in the liver.

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