Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells

Earlier, we proposed the “mechanosome” concept as a testable model for understanding how mechanical stimuli detected by cell surface adhesion molecules are transmitted to modulate gene expression inside cells. Here, for the first time we document a putative mechanosome involving Src, Pyk2 and MBD2 in MLO-Y4 osteocytes with high spatial resolution using FRET-FLIM. Src-Pyk2 complexes were concentrated at the periphery of focal adhesions and the peri-nuclear region. Pyk2-MBD2 complexes were located primarily in the nucleus and peri-nuclear region. Lifetime measurements indicated that Src and MBD2 did not interact directly. Finally, mechanical stimulation by fluid flow induced apparent accumulation of Src-Pyk2 protein complexes in the peri-nuclear/nuclear region, consistent with the proposed behavior of a mechanosome in response to a mechanical stimulus.

Snapping out of Autopilot: Overriding Habits in Real Time and the Role of Ventrolateral Prefrontal Cortex

Habits allow environmental and interoceptive cues to trigger behavior in an automatized fashion, making them liable to deployment in inappropriate or outdated contexts. Over the long-term, repeated failure of a once adaptive habit to satisfy current goals produces extinction learning that suppresses the habit’s execution. Less attention has been afforded to the mechanisms underlying real-time habit suppression: the capacity to stop the execution of a cued habit that is goal-conflicting. Here, we first posit a model by which goal-relevant stimuli can 1) bring unfolding habits and their projected outcomes into awareness, 2) prompt evaluation of the habit outcome with respect to current goals, and 3) trigger cessation of the habit response if it is determined to be goal-conflicting. Second, we propose a modified stop-signal task to test this model of “goal-directed stopping of habit execution”. Finally, we marshal evidence indicating that the ventrolateral prefrontal cortex (vlPFC), situated at the nexus of salience detection, action-plan assessment, and motor inhibition networks, is uniquely positioned to coordinate the overriding of habitual behaviors in real time. In sum, this review presents a testable model and candidate neurobiological substrate for our capacity to “snap out of autopilot” and override goal-conflicting habits in real time.

Branding of Ternate City

City branding has become a massive effort by many cities as tourist destinations to promote the city nationally and internationally. Ambon City, the Capital of Maluku Province, known as the “City of Music,” and the City of Solo with “Solo the Spirit of Java,” are examples of city branding. In North Maluku Province, Ternate City has a long history of colonialism, international trade, and a developing city. Unfortunately, inconsistencies still exist in how the city should be known. This study aims to identify and define city branding by using the constructivist grounded theory method. The themes identified were quality tourism, economic value, community engagement, stakeholder consensus, legendary ancient leadership, and historic and renowned commodity. Six main themes are theoretically based on how to branding Ternate city and involving seven important participants. These themes are then used to define the branding of Ternate City. Current research offers a testable model to brand a place or city based on the identified subjects. At the same time, potential future studies are also suggested regarding the weaknesses of the current study.

Workplace Hazing: Toward an Organizational Science of a Cryptic Group Practice

The purpose of this paper is to establish a foundation for studying and managing new employee hazing in workgroups. Available empirical evidence indicates 25–75% of American employees encounter workplace hazing, but very little empirical research exists on this phenomenon. Workers are changing jobs more frequently than ever, which increases the cumulative impact and importance of new employee experiences, including hazing, a complex group-based phenomenon. Because hazing is a relatively universal social practice without a strongly established literature in the organizational sciences, we draw from multiple disciplines in reviewing and modeling the practice. The current research offers three major contributions: (a) a relatively exhaustive review of relevant empirical and theoretical work on hazing, (b) an initial, testable model for understanding workplace hazing as a multi-level phenomenon, including individual and group-level antecedents and outcomes, and (c) an outline of the need and support for considering both the dysfunctional and functional consequences of hazing, given the variety of forms it takes and reactions it evokes. Finally, we present actionable guidance for researchers seeking to study workplace hazing and discuss the organizational implications of our work for practitioners.

CANUE: A Theoretical Model of Pain as an Antecedent for Substance Use

Background Pain and substance use are frequently comorbid and have been shown to exert bidirectional effects. Self-medication of pain and distress via substance use is common and can be understood via negative reinforcement, ultimately strengthening the pathway between pain to substance use over time. As such, a testable model of the potentially modifiable candidate mechanisms that underlie the pain to substance use pathway is needed. Purpose This review proposes a testable model of pain as an antecedent to substance use to guide future research and inform clinical practice. Methods An integrative review of current evidence regarding pain, substance use, and associated risk factors (i.e., negative affect, pain-related attitudes, negative urgency, and substance use outcome expectancies) was conducted. Results The Catastrophizing, Anxiety, Negative Urgency, and Expectancy (CANUE) model highlights modifiable risk factors for self-medicating pain with substance use, including increased negative affect and maladaptive pain-related attitudes (i.e., pain catastrophizing, pain anxiety, and fear of pain), negative urgency, and substance-related outcome expectancies for pain relief and enhanced pain coping. Conclusions Targeted behavioral and psychological interventions that address these factors may facilitate more adaptive pain-coping responses, thereby reducing the impacts of pain on substance use. Systematic research is needed to evaluate the validity and clinical utility of this model.

Houston: 1969

Dr. Denton Cooley’s patient Haskell Karp awaits a heart transplant and is offered a temporary artificial heart instead. The chapter recapitulates the efforts of the DeBakey lab in artificial heart experiments and the development of testable model. Cooley and DeBakey lab technician Domingo Liotta conspire to hijack an artificial heart model. Implantation of the device occurs in Karp, followed by pleas for human donor. Baylor investigates the circumstances of the Karp operation and the procuring of an artificial heart. Following the investigation, Liotta is dismissed and Cooley resigns. This is the beginning of a four-decade estrangement between DeBakey and Cooley.

Above and beyond the concrete: The diverse representational substrates of the predictive brain

In recent years, scientists have increasingly taken to investigate the predictive nature of cognition. We argue that prediction relies on abstraction, and thus theories of predictive cognition need an explicit theory of abstract representation. We propose such a theory of the abstract representational capacities that allow humans to transcend the “here-and-now.” Consistent with the predictive cognition literature, we suggest that the representational substrates of the mind are built as a hierarchy, ranging from the concrete to the abstract; however, we argue that there are qualitative differences between elements along this hierarchy, generating meaningful, often unacknowledged, diversity. Echoing views from philosophy, we suggest that the representational hierarchy can be parsed into: modality-specific representations, instantiated on perceptual similarity; multimodal representations, instantiated primarily on the discovery of spatiotemporal contiguity; and categorical representations, instantiated primarily on social interaction. These elements serve as the building blocks of complex structures discussed in cognitive psychology (e.g., episodes, scripts) and are the inputs for mental representations that behave like functions, typically discussed in linguistics (i.e., predicators). We support our argument for representational diversity by explaining how the elements in our ontology are all required to account for humans’ predictive cognition (e.g., in subserving logic-based prediction; in optimizing the trade-off between accurate and detailed predictions) and by examining how the neuroscientific evidence coheres with our account. In doing so, we provide a testable model of the neural bases of conceptual cognition and highlight several important implications to research on self-projection, reinforcement learning, and predictive-processing models of psychopathology.

The Message Shapes Phonology

Based on a diverse and complementary set of theoretical and empirical findings, we describe an approach to phonology in which sound patterns are shaped by the trade-off between biases supporting message transmission accuracy and resource cost. We refer to this approach as Message-Oriented Phonology. The evidence suggests that these biases influence the form of messages, defined with reference to a language's morphemes, words or higher levels of meaning, rather than influencing phonological categories directly. Integrating concepts from information theory and Bayesian inference with the existing body of phonological research, we propose a testable model of phonology that makes quantitative predictions. Moreover, we show that approaching language as a system of message transfer provides greater explanatory coverage of a diverse range of sound patterns.