scholarly journals Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261660
Author(s):  
Richard N. Day ◽  
Kathleen H. Day ◽  
Fredrick M. Pavalko
Keyword(s):  
High Spatial Resolution ◽  
Protein Complexes ◽  
Focal Adhesions ◽  
Mechanical Stimulus ◽  
Mechanical Stimuli ◽  
Nuclear Region ◽  
Surface Adhesion ◽  
Lifetime Measurements ◽  
First Time ◽  
Testable Model

Earlier, we proposed the “mechanosome” concept as a testable model for understanding how mechanical stimuli detected by cell surface adhesion molecules are transmitted to modulate gene expression inside cells. Here, for the first time we document a putative mechanosome involving Src, Pyk2 and MBD2 in MLO-Y4 osteocytes with high spatial resolution using FRET-FLIM. Src-Pyk2 complexes were concentrated at the periphery of focal adhesions and the peri-nuclear region. Pyk2-MBD2 complexes were located primarily in the nucleus and peri-nuclear region. Lifetime measurements indicated that Src and MBD2 did not interact directly. Finally, mechanical stimulation by fluid flow induced apparent accumulation of Src-Pyk2 protein complexes in the peri-nuclear/nuclear region, consistent with the proposed behavior of a mechanosome in response to a mechanical stimulus.

scholarly journals Direct observation of a coil-to-helix contraction triggered by vinculin binding to talin

2020 ◽  
Vol 6 (21) ◽  
pp. eaaz4707 ◽  
Author(s):  
Rafael Tapia-Rojo ◽  
Alvaro Alonso-Caballero ◽  
Julio M. Fernandez
Keyword(s):  
Focal Adhesions ◽  
Interaction Force ◽  
Magnetic Tweezers ◽  
Feedback Mechanism ◽  
Force Transmission ◽  
Mechanical Coupling ◽  
Negative Feedback Mechanism ◽  
The Reformation ◽  
Energy Penalty ◽  
First Time

Vinculin binds unfolded talin domains in focal adhesions, which recruits actin filaments to reinforce the mechanical coupling of this organelle. However, it remains unknown how this interaction is regulated and its impact on the force transmission properties of this mechanotransduction pathway. Here, we use magnetic tweezers to measure the interaction between vinculin head and the talin R3 domain under physiological forces. For the first time, we resolve individual binding events as a short contraction of the unfolded talin polypeptide caused by the reformation of the vinculin-binding site helices, which dictates a biphasic mechanism that regulates this interaction. Force favors vinculin binding by unfolding talin and exposing the vinculin-binding sites; however, the coil-to-helix contraction introduces an energy penalty that increases with force, defining an optimal binding regime. This mechanism implies that the talin-vinculin-actin association could operate as a negative feedback mechanism to stabilize force on focal adhesions.

scholarly journals Recent behaviour of Glaciar Upsala, a fast-flowing calving glacier in Lago Argentino, southern Patagonia

2003 ◽  
Vol 36 ◽  
pp. 184-188 ◽  
Author(s):  
Pedro Skvarca ◽  
Bruce Raup ◽  
Hernan de Angelis
Keyword(s):  
Cross Correlation ◽  
High Spatial Resolution ◽  
Winter Period ◽  
Optical Images ◽  
Southern Patagonia ◽  
Glacier Front ◽  
Calving Rate ◽  
Depth Relationship ◽  
Surface Ice ◽  
First Time

AbstractSequential optical images of high spatial resolution were used for the first time to derive surface ice velocities of Glaciar Upsala, a fast-moving fresh-water calving glacier in southern Patagonia. Cross-correlation methods applied to four Landsat ETM+ images acquired in 2000–01 yielded average velocities of around 1600 m a−1, similar to values measured in the field in November 1993. The derived velocities show almost no seasonal variation for the analyzed calving termini. During the period of satellite coverage, clear readvances were detected in the autumn–winter period, followed by recessions during summers. Between 24 April 1999 and 14 October 2001, the glacier front has been fluctuating seasonally within about 400 m, in contrast to the previous dramatic recession. During the last 2.5 years, Glaciar Upsala west terminus had a net advance of around 300 m. In addition, the available satellite images allowed us to determine recent calving speeds and confirm the improved calving-rate/water-depth relationship, recently proposed by incorporating new data from Patagonian glaciers.

The initiation of nerve impulses by mesenteric Pacinian corpuscles

1950 ◽  
Vol 137 (886) ◽  
pp. 96-114 ◽  
Keyword(s):  
Conditioning Stimulus ◽  
Mechanical Stimulus ◽  
Constant Current ◽  
Mechanical Stimuli ◽  
Pacinian Corpuscles ◽  
Nerve Impulses ◽  
A Value ◽  
Test Shock ◽  
Long Duration ◽  
Refractory State

A preparation of a single Pacinian corpuscle in the cat’s mesentery has been used to study the initiation of nerve impulses in sensory endings. The minimum movement of a mechanical stimulator required to excite a single corpuscle has been found to be 0⋅5 μ in 100 μ sec. It has been difficult to produce repetitive discharges with rectangular pulses of long duration, either mechanical or of constant current. The latency between a mechanical stimulus and the initiation of an impulse has a value around 1⋅5 msec, for threshold stimuli, and this decreases to a minimum value around 0⋅5 msec, as the stimulus is increased; it is altered only slightly, if at all, by changes in the duration of the maintained displacement of the mechanical stimulator. Subthreshold mechanical stimuli have been shown to facilitate stimulation by electrical test shocks. The return of excitability at the ending is independent of the nature of the conditioning stimulus and varies but little with the nature of the test shock. The value of the latency at threshold is unaffected by the relatively refractory state. The relations of these results to various hypotheses are discussed, and it is suggested that these results can all be accounted for in terms of the known properties of axons.

Rapid Contractions and Associated Potentials in a Sand-Dwelling Anemone

1969 ◽  
Vol 51 (2) ◽  
pp. 513-528
Author(s):  
PETER E. PICKENS
Keyword(s):  
Maximum Size ◽  
Mechanical Stimulus ◽  
Retractor Muscle ◽  
Mechanical Stimuli ◽  
Nerve Impulses ◽  
Electrical Potentials ◽  
Spread Of Excitation ◽  
Electrical Stimuli ◽  
Withdrawal Response ◽  
Muscle Potential

1. Two kinds of electrical potentials can be recorded from the surface of the. retractor muscle of the anemone, Calamactis, during rapid contraction. These are large muscle action potentials and smaller pulses which are thought to be nerve spikes The latter resemble nerve impulses of higher organisms in that they are all-or-none and of short duration. 2. A nerve spike follows each of a pair of electrical stimuli, but the muscle potential and contraction occur only after the second shock, indicating that facilitation is required at the neuromuscular junction. 3. The size of the muscle potential and of the contraction are correlated with the interval between paired electrical stimuli. Maximum size is reached when stimuli are zoo msec. apart even though the minimum effective interval is 30 msec. 4. A muscle potential precedes contraction only along the upper part of the retractor muscle and this is the part that contracts rapidly during the withdrawal response. The lower retractor does not contract. 5. Conduction velocity along the upper retractor is higher than along the lower. The histological correlate of rapid conduction is a nerve net with large, long, longitudinally oriented fibres. 6. The refractory period of the conducting system of the upper retractor is shorter than that of the lower retractor. Consequently, spread of excitation toward the aboral end is limited if paired stimuli are further apart than 250-300 msec. 7. A mechanical stimulus which is just strong enough to elicit a withdrawal response evokes a single muscle potential of maximum size, suggesting that two nerve impulses closer together than 200 msec. precede the muscle potential. Stronger mechanical stimuli evoke a burst of muscle potentials.

Ice drift in Peter the Great Bay

2014 ◽  
Vol 178 (3) ◽  
pp. 148-156
Author(s):  
Vyacheslav A. Dubina ◽  
Vladimir V. Plotnikov ◽  
Nina S. Kot
Keyword(s):  
Satellite Images ◽  
High Spatial Resolution ◽  
Peter The Great Bay ◽  
Western Coast ◽  
Mean Values ◽  
Peter The Great ◽  
Great Bay ◽  
Ice Drift ◽  
The Right ◽  
First Time

Dynamics of the sea ice cover in Peter the Great Bay is considered, for the first time for its whole area, on the base of satellite images received in 2004-2011 from the spectroradiometers MODIS mounted on the satellites Terra and Aqua. High spatial resolution maps of the ice drift are constructed for various wind conditions. Mean values of the drift velocity and wind coefficient are calculated for four parts of the Bay. In usual conditions of winter monsoon, the ice in the central part of Peter the Great Bay drifts southward with the velocity 0.5-0.6 m/s with deviation from the wind direction about 40° to the right; the ice at the western coast drifts along the island chain with the velocity 0.1-0.4 m/s under wind of any direction in the quadrant from northwest to northeast.

scholarly journals EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

2017 ◽  
Vol 216 (10) ◽  
pp. 3179-3198 ◽  
Author(s):  
Chao Yang ◽  
Jingchao Wu ◽  
Cecilia de Heus ◽  
Ilya Grigoriev ◽  
Nalan Liv ◽  
...  
Keyword(s):  
Cell Division ◽  
Cell Lines ◽  
Protein Complexes ◽  
Focal Adhesions ◽  
Microtubule Organization ◽  
The Core ◽  
Microtubule Polymerization ◽  
Golgi Membranes ◽  
Core Components ◽  
Knockout Technology

End-binding proteins (EBs) are the core components of microtubule plus end tracking protein complexes, but it is currently unknown whether they are essential for mammalian microtubule organization. Here, by using CRISPR/Cas9-mediated knockout technology, we generated stable cell lines lacking EB2 and EB3 and the C-terminal partner-binding half of EB1. These cell lines show only mild defects in cell division and microtubule polymerization. However, the length of CAMSAP2-decorated stretches at noncentrosomal microtubule minus ends in these cells is reduced, microtubules are detached from Golgi membranes, and the Golgi complex is more compact. Coorganization of microtubules and Golgi membranes depends on the EB1/EB3–myomegalin complex, which acts as membrane–microtubule tether and counteracts tight clustering of individual Golgi stacks. Disruption of EB1 and EB3 also perturbs cell migration, polarity, and the distribution of focal adhesions. EB1 and EB3 thus affect multiple interphase processes and have a major impact on microtubule minus end organization.

3D ionization structure and kinematics of NGC 2392

2011 ◽  
Vol 7 (S283) ◽  
pp. 348-349
Author(s):  
Reginald J. Dufour ◽  
Jonathan N. Sick ◽  
Patrick M. Hartigan ◽  
Richard B. C. Henry ◽  
Karen B. Kwitter ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Velocity Structure ◽  
High Spatial Resolution ◽  
Emission Lines ◽  
3 Dimensional ◽  
3D Morphology ◽  
Ionization Structure ◽  
First Time ◽  
Echelle Spectroscopy

AbstractWe discuss the 3D morphology, ionization structure, and kinematics of NGC 2392, the “Eskimo,” based on new and archival HST imagery and new long-slit echelle spectroscopy. High spatial resolution ionization maps of the nebula were made from HST WFPC2 imagery and compared with their velocity structure in various emission lines from echelle spectra taken with the 4m telescope at Kitt Peak. The imagery and spectra were then compared to map the kinematics of the nebula in several emission lines and decode the 3-dimensional morphology and ionization structure of the nebula, including that of C+2 from C III] 1909 Å for the first time.

scholarly journals Shadow monochromatic backlighting: Large-field high resolution X-ray shadowgraphy with improved spectral tunability

2001 ◽  
Vol 19 (2) ◽  
pp. 285-293 ◽  
Author(s):  
T.A. PIKUZ ◽  
A. YA. FAENOV ◽  
M. FRAENKEL ◽  
A. ZIGLER ◽  
F. FLORA ◽  
...  
Keyword(s):  
High Resolution ◽  
High Spatial Resolution ◽  
Field Of View ◽  
Large Field ◽  
X Ray ◽  
Traditional Scheme ◽  
Wide Range ◽  
Wide Wavelength Range ◽  
Bent Crystals ◽  
First Time

The shadow monochromatic backlighting (SMB) scheme, a modification of the well-known soft X-ray monochromatic backlighting scheme, is proposed. It is based on a spherical crystal as the dispersive element and extends the traditional scheme by allowing one to work with a wide range of Bragg angles and thus in a wide spectral range. The advantages of the new scheme are demonstrated experimentally and supported numerically by ray-tracing simulations. In the experiments, the X-ray backlighter source is a laser-produced plasma, created by the interaction of an ultrashort pulse, Ti:Sapphire laser (120 fs, 3–5 mJ, 1016 W/cm2 on target) or a short wavelength XeCl laser (10 ns, 1–2 J, 1013 W/cm2 on target) with various solid targets (Dy, Ni + Cr, BaF2). In both experiments, the X-ray sources are well localized spatially (∼20 μm) and are spectrally tunable in a relatively wide wavelength range (λ = 8–15 Å). High quality monochromatic (δλ/λ ∼ 10−5–10−3) images with high spatial resolution (up to ∼4 μm) over a large field of view (a few square millimeters) were obtained. Utilization of spherically bent crystals to obtain high-resolution, large field, monochromatic images in a wide range of Bragg angles (35° < Θ < 90°) is demonstrated for the first time.

scholarly journals Control of cellular responses to mechanical cues through YAP/TAZ regulation

2019 ◽  
Vol 294 (46) ◽  
pp. 17693-17706 ◽  
Author(s):  
Ishani Dasgupta ◽  
Dannel McCollum
Keyword(s):  
Cell Fate ◽  
Wound Repair ◽  
Hippo Pathway ◽  
Three Dimensional ◽  
Focal Adhesions ◽  
Mechanical Stimuli ◽  
Cell Contractility ◽  
Cell Fate Decisions ◽  
Disease States ◽  
The Hippo Pathway

To perceive their three-dimensional environment, cells and tissues must be able to sense and interpret various physical forces like shear, tensile, and compression stress. These forces can be generated both internally and externally in response to physical properties, like substrate stiffness, cell contractility, and forces generated by adjacent cells. Mechanical cues have important roles in cell fate decisions regarding proliferation, survival, and differentiation as well as the processes of tissue regeneration and wound repair. Aberrant remodeling of the extracellular space and/or defects in properly responding to mechanical cues likely contributes to various disease states, such as fibrosis, muscle diseases, and cancer. Mechanotransduction involves the sensing and translation of mechanical forces into biochemical signals, like activation of specific genes and signaling cascades that enable cells to adapt to their physical environment. The signaling pathways involved in mechanical signaling are highly complex, but numerous studies have highlighted a central role for the Hippo pathway and other signaling networks in regulating the YAP and TAZ (YAP/TAZ) proteins to mediate the effects of mechanical stimuli on cellular behavior. How mechanical cues control YAP/TAZ has been poorly understood. However, rapid progress in the last few years is beginning to reveal a surprisingly diverse set of pathways for controlling YAP/TAZ. In this review, we will focus on how mechanical perturbations are sensed through changes in the actin cytoskeleton and mechanosensors at focal adhesions, adherens junctions, and the nuclear envelope to regulate YAP/TAZ.

Homing and home range of the European spiny lobster, Palinurus elephas (Decapoda, Palinuridae) acoustically tracked

Crustaceana ◽  
2019 ◽  
Vol 92 (4) ◽  
pp. 463-476
Author(s):  
Vincenzo M. Giacalone ◽  
Arturo Zenone ◽  
Fabio Badalamenti ◽  
Javier Ciancio ◽  
Gaspare Buffa ◽  
...  
Keyword(s):  
Home Range ◽  
Protected Area ◽  
Marine Protected Area ◽  
High Spatial Resolution ◽  
Spiny Lobster ◽  
Ultrasonic Telemetry ◽  
Central Mediterranean ◽  
In The Wild ◽  
First Time ◽  
Palinurus Elephas

Abstract A specific study has been carried out for the first time to investigate the homing capability and daily home range of the spiny lobster Palinurus elephas by means of ultrasonic telemetry. Nine lobsters collected in the Capo Gallo — Isola delle Femmine marine protected area (northwestern Sicily, central Mediterranean) were tagged with miniaturized transmitters and released at a single site inside the protected area. The lobsters were monitored with the purpose of calculating their horizontal and vertical positions, analysing their movement patterns to assess their homing capability, and calculating their daily home range. Five lobsters moved back close to the capture sites within the first 20 hours after release (‘homed’). The remaining four lobsters ‘relocated’ to a different refuge. Homed lobsters had a larger home range than relocated lobsters. This study provides the first description of a homing pattern with high spatial resolution in the wild European spiny lobster as inferred by ultrasonic telemetry.

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