Cell Detachment | ScienceGate

Within the last years a comprehensive number of scientific studies demonstrated beneficial effect of Arthropira platensis (AP) as dietary supplement due to a high content of proteins, minerals and vitamins. Positive effects like promoting the immune system, reducing inflammation and an anti-oxidant capacity are reported. In this study, the effect of an aqueous AP extract on primary human venous endothelial cells (HUVEC) was investigated. In addition, the effect of AP on HUVEC treated with a bacterial toxin (lipopolysaccharide, LPA), inducing an activation of HUVEC and cellular detachment, was analyzed. Depending on the concentration of AP extract a significantly accelerated formation of an endothelial cell monolayer was observed. Furthermore, the detachment of HUVEC after LPA addition was dramatically reduced by AP. In conclusion, the data are promising and indicatory for an application of Arthrospira platensis in the clinical field.

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INHIBITION OF PROTEIN KINASE C EPSILON CAUSES CILIATED BOVINE BRONCHIAL CELL DETACHMENT

Experimental Lung Research ◽

10.1080/01902140600959630 ◽

2006 ◽

Vol 32 (8) ◽

pp. 349-362 ◽

Cited By ~ 10

Author(s):

Rebecca E. Slager ◽

Joseph H. Sisson ◽

Jaqueline A. Pavlik ◽

Jennifer K. Johnson ◽

Jeremy R. Nicolarsen ◽

...

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Cell Detachment ◽

Kinase C ◽

Protein Kinase C Epsilon

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Characterisation of Cell Adhesion to Substrate Materials and the Resistance to Enzymatic and Mechanical Cell-Removal

MRS Proceedings ◽

10.1557/proc-1097-gg03-05 ◽

2008 ◽

Vol 1097 ◽

Cited By ~ 2

Author(s):

Helen Jane Griffiths ◽

John G Harvey ◽

James Dean ◽

James A Curran ◽

Athina E Markaki ◽

...

Keyword(s):

Fluid Dynamics ◽

Computational Fluid Dynamics ◽

Cell Adhesion ◽

Liquid Flow ◽

Adhesive Strength ◽

Cell Detachment ◽

Shear Forces ◽

Cfd Modelling ◽

Computational Fluid Dynamics Cfd

AbstractCell-implant adhesive strength is important for prostheses. In this paper, an investigation is described into the adhesion of bovine chondrocytes to Ti6Al4V-based substrates with different surface roughnesses and compositions. Cells were cultured for 2 or 5 days, to promote adhesion. The ease of cell removal was characterised, using both biochemical (trypsin) and mechanical (accelerated buoyancy and liquid flow) methods. Computational fluid dynamics (CFD) modelling has been used to estimate the shear forces applied to the cells by the liquid flow. A comparison is presented between the ease of cell detachment indicated using these methods, for the three surfaces investigated.

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Cell cycle regulation of mammary epithelial cell detachment byStaphylococcus aureus

Journal of Dairy Research ◽

10.1017/s0022029900032088 ◽

1996 ◽

Vol 63 (4) ◽

pp. 543-553 ◽

Cited By ~ 1

Author(s):

Boris Zavizion ◽

Andrew J. Bramley ◽

Ioannis Politis

Keyword(s):

Cell Cycle ◽

Epithelial Cells ◽

Culture Medium ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

Cell Detachment ◽

Staph Aureus ◽

Bovine Mammary Epithelial Cells ◽

Isogenic Mutants ◽

Cycle Regulation

SummaryThe effect ofStaphylococcus aureuson detachment of bovine mammary epithelial cells in culture was examined. Mammary epithelial cells became detached from fresh monolayers following a 3 h incubation in the presence ofStaph. aureusM60. Two different procedures indicated that cell detachment coincided with the S-phase of the cell cycle. The roles of proteinases, toxins and Ca availability in inducing cell detachment were examined. Addition of the proteinase inhibitor phenyl-methylsulphonyl fluoride (1 mM) to the culture medium prevented cell detachment. Addition of a combination of purified staphylococcal proteinases XVI and XVII-B to the culture medium of mammary epithelial cells induced cell detachment in the absence ofStaph. aureus. Cell detachment may be caused by a staphylococcal proteinase. However, addition of Ca (10 mM) to the culture medium abolishedStaph. aureus-induced cell detachment, despite the fact that proteinase activity was still apparently present. Isogenic mutants ofStaph. aureusM60, expressing either ± or β toxins but not both, induced cell detachment, but to a lesser extent than the wild type. Thus, Ca and toxins play some role during cell detachment. Clones established from detached cells that were washed and replated showed the same susceptibility toStaph. aureus-induced cell detachment as the parental cells. This indicated that there is no subclone of mammary epithelial cells more sensitive to this effect.

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