Biosynthesis of protein by microscopic fungi in solid state fermentation. II. Optimization of aspergillus oryzae A. or. 11 cultivation for protein enrichment of starchy raw materials

Rice bran is a primary by-product of the traditional rice processing industry. Once oil is extracted from rice bran, the left over residue is called de oiled rice bran (DOB). Its disposal presents economic and environmental problems. Microbial conversion of DOB into single cell protein (SCP) is an innovative practical approach for protein supplementation of a staple diet. The present research was undertaken to test the growth of industrially important fungi, Aspergillus oryzae MTCC 1846 on DOB through solid state fermentation (SSF) technology. Various process parameters effect such as moisture, pH of the substrate, inoculum size, temperature and nitrogen source for maximum protein enrichment were studied. The optimum conditions for the enrichment process were found to be moisture content 60%; temperature 280C; pH 6.0; inoculums concentration 109 spores/ g substrate and particle size of DOB, 0.3 mm. Among the various nitrogen source tested, ammonium sulfate (0.6% w/w) showed maximum protein enrichment (24.30%) followed by vegetable + fruit waste extract (23.50%) and legume root extract (23.10%).

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Optimization of Cultural Conditions for Solid State Fermentation of Amylase Production by Aspergillus species

Nepal Journal of Science and Technology ◽

10.3126/njst.v14i1.8924 ◽

2013 ◽

Vol 14 (1) ◽

pp. 67-74

Author(s):

Bina Gautam ◽

Tika B Karki ◽

Om Prakash Panta

Keyword(s):

Solid State ◽

Rice Bran ◽

Solid State Fermentation ◽

Food Industry ◽

Raw Materials ◽

Morphological Characteristics ◽

Amylolytic Enzyme ◽

Cultural Conditions ◽

Amylase Production ◽

Selection Of

Amylase is an amylolytic enzyme used in food industry which is generally produced by Aspergillus spp. under solid state fermentation. The present study is concerned with the isolation, screening and selection of suitable strains of Aspergillus spp. and optimization of cultural conditions for the biosynthesis of amylase. Rice and wheat brans were used as substrates which are readily available inexpensive raw materials for amylase production. From 85 samples of rice and wheat grains, 55 colonies obtained on potato dextrose agar (PDA) were suspected to be Aspergillus oryzae and only 35 colonies possessed the morphological characteristics similar to that of A. oryzae indicating the isolates were most likely the strains of A. oryzae. Of all the fungal isolates of Aspergillus spps., Asp.31 gave maximum production of amylase (720.782 IUgds-1) in solid state fermentation media. This strain was selected as a parental strain for optimization for cultural conditions. The obtained data were analyzed using SPSS- 11.5 program. Of all the substrates (rice bran, wheat bran and their mixture), rice bran was the best for producing amylase of highest activity 611.614 IUgds-1.The highest enzyme activity of 698.749 IUgds-1 was observed at 50% initial moisture level of the substrate. The optimum temperature was 25°C for producing the crude amylase enzyme with amylase activity of 577.757 IUgds-1. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 67-74 DOI: http://dx.doi.org/10.3126/njst.v14i1.8924

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PENGARUH UKURAN PARTIKEL AMPAS SAGU DALAM PRODUKSI PIGMEN ANGKAK MENGGUNAKAN Monascus purpureus

Jurnal Teknologi Pertanian Andalas ◽

10.25077/jtpa.22.2.111-117.2018 ◽

2018 ◽

Vol 22 (2) ◽

pp. 111

Author(s):

Alfi Asben ◽

Deivy Andhika Permata

Keyword(s):

Particle Size ◽

Solid State ◽

Solid State Fermentation ◽

Raw Materials ◽

Rice Flour ◽

Monascus Purpureus ◽

Color Intensity ◽

Food Colorants

Angka pigment is one of food colorants that safe to used. It can be produced by subtrate that contain of sago hampas. The objective of the research was to get the appropriate of sago hampas particle size to produce the angkak pigment. The steps to produce of angkak pigment were (a) Preparation of raw materials (sago hampas and rice flour substrate with comparison 1:1 (12.5 : 12.5). This research used three treatments of sago hampas particle size (40-60 mesh, 60-80 mesh, and >80 mesh) with 3 replications, (b) Preparation of Monascus purpureus culture, (c) Solid state fermentation to produce angkak pigment using M. purpureus. The results of the research showed that the substrate with hampas sago particle size 40-60 mesh produced the best angkak pigment. The angkak pigment obtain the highest color intensity on λ 400 nm, λ 470 nm, λ 500 nm were 6004, 5110 and 3650 respectively, the highest used starch, antioxidant, toxicity, lovastatin and spore of M. purpureus were 11.07%, 45.95%, 1719.86 (LC50), 79 ppm, and 3.4 x 103 CPU/g respectively.

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Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

Waste Management & Research ◽

10.1177/0734242x18798699 ◽

2018 ◽

Vol 37 (2) ◽

pp. 149-156 ◽

Cited By ~ 12

Author(s):

C. Marzo ◽

A.B. Díaz ◽

I. Caro ◽

A. Blandino

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Reducing Sugars ◽

Raw Materials ◽

Hydrolytic Enzymes ◽

Industrial Wastes ◽

Added Value ◽

Orange Peels ◽

Enzyme Cocktail ◽

Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

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TECHNOLOGY OF PLANT RAW MATERIALS MICROBIOLOGICAL PROCESSING WITH PLEUROTUS STRAINS FOR FEED PRODUCTS PRODUCTION

chemistry of plant raw material ◽

10.14258/jcprm.2020048445 ◽

2020 ◽

pp. 405-414

Author(s):

Veronika Valentinovna Tarnopol’skaya ◽

Tat'yana Vasil'yevna Ryazanova ◽

Natal'ya Yur'yevna Demidenko ◽

Oksana Nikolayevna Eryomenko

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Solid Phase ◽

Raw Materials ◽

Jerusalem Artichoke ◽

Seed Stock ◽

Raw Material ◽

Pine Sawdust ◽

Plant Raw Materials ◽

Pure Cultures

A technology for pilot production of feed products via microbiological conversion of plant raw materials (mixed substrate of pine sawdust and vegetative part of Jerusalem artichoke) by Plerotus ostreatus PO-4.1 and Pleurotus djamor PD-3.2 strains is developed. The technology includes hydrodynamic activation of substrate at the seed stock production stage. The overall technology includes three key stages: submerged fermentation of pure cultures of production strains; submerged-solid phase fermentation of hydrodynamicly activated plant raw materials for seed stock production; solid-state fermentation of mechanically ground plant substrate for feed products production. A successful approbation of submerged-solid state fermentation of production strains on media containing 3% of hydrodynamicly activated raw materials allowed for obtaining seed stock with 14.5 g/l yield of submerged mycelium biomass fully adopted for this type of substrate. Further use of this seed stock biomass at the solid state fermentation stage makes the overall process duration three times shorter compared to existing technologies for direct wood waste bioconversion. The pilot plant results show valuable practicability of plant raw material hydrodynamic activation with the purpose of enhancing its bioaccessibility with consequent increase in degree of microbiological conversion. The product of bioconversion contains 14–16% of protein, biofiber, vitamins and minerals and could be considered for successful use as feed by agricultural enterprises.

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