Improving livestock for agriculture - technological progress from random transgenesis to precision genome editing heralds a new era

2014 ◽  
Vol 10 (1) ◽  
pp. 109-120 ◽  
Author(s):  
Götz Laible ◽  
Jingwei Wei ◽  
Stefan Wagner
2021 ◽  
Vol 22 (10) ◽  
pp. 5167
Author(s):  
Lei Liu ◽  
Penelope L. Lindsay ◽  
David Jackson

Artificial domestication and improvement of the majority of crops began approximately 10,000 years ago, in different parts of the world, to achieve high productivity, good quality, and widespread adaptability. It was initiated from a phenotype-based selection by local farmers and developed to current biotechnology-based breeding to feed over 7 billion people. For most cereal crops, yield relates to grain production, which could be enhanced by increasing grain number and weight. Grain number is typically determined during inflorescence development. Many mutants and genes for inflorescence development have already been characterized in cereal crops. Therefore, optimization of such genes could fine-tune yield-related traits, such as grain number. With the rapidly advancing genome-editing technologies and understanding of yield-related traits, knowledge-driven breeding by design is becoming a reality. This review introduces knowledge about inflorescence yield-related traits in cereal crops, focusing on rice, maize, and wheat. Next, emerging genome-editing technologies and recent studies that apply this technology to engineer crop yield improvement by targeting inflorescence development are reviewed. These approaches promise to usher in a new era of breeding practice.


Author(s):  
Akshatha Desai ◽  
Naicy Thomas ◽  
Vasudhar Bhat ◽  
Akhil H ◽  
Aravindakshan V
Keyword(s):  

2019 ◽  
Vol 7 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Qamar U. Zaman ◽  
Chao Li ◽  
Hongtao Cheng ◽  
Qiong Hu

2017 ◽  
Vol 37 (01) ◽  
pp. 45-52 ◽  
Author(s):  
Simone Haas ◽  
Viviane Dettmer ◽  
Toni Cathomen

SummaryTargeted genome editing with designer nucleases, such as zinc finger nucleases, TALE nucleases, and CRISPR-Cas nucleases, has heralded a new era in gene therapy. Genetic disorders, which have not been amenable to conventional gene-addition-type gene therapy approaches, such as disorders with dominant inheritance or diseases caused by mutations in tightly regulated genes, can now be treated by precise genome surgery. Moreover, engineered nucleases enable novel genetic interventions to fight infectious diseases or to improve cancer immunotherapies. Here, we review the development of the different classes of programmable nucleases, discuss the challenges and improvements in translating gene editing into clinical use, and give an outlook on what applications can expect to enter the clinic in the near future.


eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Paul McVeigh ◽  
Aaron G Maule

The first reports of CRISPR/Cas9 genome editing in flatworms could usher in a new era of research on these dangerous human parasites.


2017 ◽  
Vol 95 (2) ◽  
pp. 187-201 ◽  
Author(s):  
Jayme Salsman ◽  
Graham Dellaire

With the introduction of precision genome editing using CRISPR–Cas9 technology, we have entered a new era of genetic engineering and gene therapy. With RNA-guided endonucleases, such as Cas9, it is possible to engineer DNA double strand breaks (DSB) at specific genomic loci. DSB repair by the error-prone non-homologous end-joining (NHEJ) pathway can disrupt a target gene by generating insertions and deletions. Alternatively, Cas9-mediated DSBs can be repaired by homology-directed repair (HDR) using an homologous DNA repair template, thus allowing precise gene editing by incorporating genetic changes into the repair template. HDR can introduce gene sequences for protein epitope tags, delete genes, make point mutations, or alter enhancer and promoter activities. In anticipation of adapting this technology for gene therapy in human somatic cells, much focus has been placed on increasing the fidelity of CRISPR–Cas9 and increasing HDR efficiency to improve precision genome editing. In this review, we will discuss applications of CRISPR technology for gene inactivation and genome editing with a focus on approaches to enhancing CRISPR–Cas9-mediated HDR for the generation of cell and animal models, and conclude with a discussion of recent advances and challenges towards the application of this technology for gene therapy in humans.


2013 ◽  
pp. 19-27
Author(s):  
Petro Sauh

The world in which we live for millennia is a breakthrough, entering into a lane of profound changes, in which the whole of our life is rebuilt and rebuilt. Untwisted to the maximum turns the flywheel of transformations has touched and is ready to deform various spheres of existence of man and humanity: the relation between humanity and the planet in which it lives; the interaction between the states, each of which is looking for its own ways to the future and, at the same time, can not but reckon with the interests of other nations and states; the struggle of social groups and the confrontation of religions, in the interrelationships of which they are struggling to realize that humanity has a common destiny and that universal values ​​and ideals must come first, become the main ones in the interaction between people; high pace of scientific and technological progress, which far from unequivocally affect both the knowledge of our lives, and on ourselves. In other words: we are faced with a new world - both in the latest technologies, in new forms of life, in new ways of worldview and world outlook, and most importantly in those global threats, in which the contradiction between the new realities of our existence and the established forms and methods unfolds. an attitude to this world. It is no coincidence that society and the church face an acute problem of adaptation to global change, the formation of a new worldview that corresponds to a new era.


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