Challenges in mass spectrometry-based quantification of bioactive peptides: A case study exploring the neuropeptide Y family

A viral development of statistical data processing, computing capabilities, chromatography-mass spectrometry, and omics technologies (technologies based on the achievements of genomics, transcriptomics, proteomics, metabolomics) in recent decades has not led to formation of a unified protocol for untargeted profiling. Systematic errors reduce the reproducibility and reliability of the obtained results, and at the same time hinder consolidation and analysis of data gained in large-scale multi-day experiments. We propose an algorithm for conducting omics profiling to identify potential markers in the samples of complex composition and present the case study of urine samples obtained from different clinical groups of patients. Profiling was carried out by the method of liquid chromatography mass spectrometry. The markers were selected using methods of multivariate analysis including machine learning and feature selection. Testing of the approach was performed using an independent dataset by clustering and projection on principal components.

Download Full-text

Direct infusion–tandem mass spectrometry combining with data mining strategies enables rapid chemome characterization of medicinal plants: A case study of Polygala tenuifolia

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2021.114281 ◽

2021 ◽

pp. 114281

Author(s):

Ting Li ◽

Zhizi Zhou ◽

Ke Zhang ◽

Wen Ma ◽

Wei Chen ◽

...

Keyword(s):

Mass Spectrometry ◽

Data Mining ◽

Medicinal Plants ◽

Tandem Mass Spectrometry ◽

Tandem Mass ◽

Direct Infusion ◽

Polygala Tenuifolia

Download Full-text

Ultrahigh Mass Accuracy in Isotope-Selective Collision-Induced Dissociation Using Correlated Sweep Excitation and Sustained Off-Resonance Irradiation: A Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Case Study on the [M + 2H]2+Ion of Bradykinin

Analytical Chemistry ◽

10.1021/ac970254b ◽

1997 ◽

Vol 69 (17) ◽

pp. 3603-3607 ◽

Cited By ~ 25

Author(s):

Albert J. R. Heck ◽

Peter J. Derrick

Keyword(s):

Mass Spectrometry ◽

Fourier Transform ◽

Cyclotron Resonance ◽

Mass Accuracy ◽

Collision Induced Dissociation ◽

Ion Cyclotron Resonance ◽

Ion Cyclotron

Download Full-text

Exosomal Cargo May Hold the Key to Improving Reproductive Outcomes in Dairy Cows

International Journal of Molecular Sciences ◽

10.3390/ijms22042024 ◽

2021 ◽

Vol 22 (4) ◽

pp. 2024

Author(s):

Natalie Turner ◽

Pevindu Abeysinghe ◽

Pawel Sadowski ◽

Murray D. Mitchell

Keyword(s):

Mass Spectrometry ◽

Dairy Cows ◽

Bioactive Peptides ◽

Cell Types ◽

Dairy Herds ◽

Dairy Farmers ◽

Early Biomarkers ◽

Health And Disease ◽

Place Of Origin ◽

Quantitative Profiling

The reproductive status of dairy cows remains a challenge for dairy farmers worldwide, with impaired fertility linked to a significant reduction in herd profitability, due in part to impaired immunity, increased metabolic pressure, and longer postpartum anestrous interval (PPAI). Exosomes are nanovesicles released from a variety of cell types and end up in circulation, and carry proteins, bioactive peptides, lipids, and nucleic acids specific to the place of origin. As such, their role in health and disease has been investigated in humans and animals. This review discusses research into exosomes in the context of reproduction in dairy herds and introduces recent advances in mass-spectrometry (MS) based proteomics that have a potential to advance quantitative profiling of exosomal protein cargo in a search for early biomarkers of cattle fertility.

Download Full-text