Optimization of an acetate reduction pathway for producing cellulosic ethanol by engineered yeast

2016 ◽  
Vol 113 (12) ◽  
pp. 2587-2596 ◽  
Author(s):  
Guo-Chang Zhang ◽  
In Iok Kong ◽  
Na Wei ◽  
Dairong Peng ◽  
Timothy L. Turner ◽  
...  
Keyword(s):  
Cellulosic Ethanol ◽  
Engineered Yeast ◽  
Acetate Reduction

scholarly journals Engineered yeast tolerance enables efficient production from toxified lignocellulosic feedstocks

2021 ◽  
Vol 7 (26) ◽  
pp. eabf7613
Author(s):  
Felix H. Lam ◽  
Burcu Turanlı-Yıldız ◽  
Dany Liu ◽  
Michael G. Resch ◽  
Gerald R. Fink ◽  
...  
Keyword(s):  
Cellulosic Ethanol ◽  
Lightweight Design ◽  
Cost Effective ◽  
Extracellular Potassium ◽  
Efficient Production ◽  
Single Strain ◽  
Lignocellulosic Feedstocks ◽  
Engineered Yeast ◽  
Fuels And Chemicals ◽  
Aldehyde Reduction

Lignocellulosic biomass remains unharnessed for the production of renewable fuels and chemicals due to challenges in deconstruction and the toxicity its hydrolysates pose to fermentation microorganisms. Here, we show in Saccharomyces cerevisiae that engineered aldehyde reduction and elevated extracellular potassium and pH are sufficient to enable near-parity production between inhibitor-laden and inhibitor-free feedstocks. By specifically targeting the universal hydrolysate inhibitors, a single strain is enhanced to tolerate a broad diversity of highly toxified genuine feedstocks and consistently achieve industrial-scale titers (cellulosic ethanol of >100 grams per liter when toxified). Furthermore, a functionally orthogonal, lightweight design enables seamless transferability to existing metabolically engineered chassis strains: We endow full, multifeedstock tolerance on a xylose-consuming strain and one producing the biodegradable plastics precursor lactic acid. The demonstration of “drop-in” hydrolysate competence enables the potential of cost-effective, at-scale biomass utilization for cellulosic fuel and nonfuel products alike.

Sud-Chemie Will Enter Cellulosic Ethanol

2010 ◽  
pp. 10072210114425
Author(s):  
Michael McCoy
Keyword(s):  
Cellulosic Ethanol

scholarly journals Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liang Sun ◽  
Jae Won Lee ◽  
Sangdo Yook ◽  
Stephan Lane ◽  
Ziqiao Sun ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cellulosic Biomass ◽  
Hemicellulose Hydrolysate ◽  
Acetyl Coa ◽  
Engineered Yeast ◽  
Fermentation Inhibitor ◽  
Acid Lactone

AbstractPlant cell wall hydrolysates contain not only sugars but also substantial amounts of acetate, a fermentation inhibitor that hinders bioconversion of lignocellulose. Despite the toxic and non-consumable nature of acetate during glucose metabolism, we demonstrate that acetate can be rapidly co-consumed with xylose by engineered Saccharomyces cerevisiae. The co-consumption leads to a metabolic re-configuration that boosts the synthesis of acetyl-CoA derived bioproducts, including triacetic acid lactone (TAL) and vitamin A, in engineered strains. Notably, by co-feeding xylose and acetate, an enginered strain produces 23.91 g/L TAL with a productivity of 0.29 g/L/h in bioreactor fermentation. This strain also completely converts a hemicellulose hydrolysate of switchgrass into 3.55 g/L TAL. These findings establish a versatile strategy that not only transforms an inhibitor into a valuable substrate but also expands the capacity of acetyl-CoA supply in S. cerevisiae for efficient bioconversion of cellulosic biomass.

Production and Application of Lignin-Based Chemicals and Materials in the Cellulosic Ethanol Production: An Overview on Lignin Closed-Loop Biorefinery Approaches

2021 ◽  
Author(s):  
Carlos Eduardo de Araújo Padilha ◽  
Cleitiane da Costa Nogueira ◽  
Bárbara Ribeiro Alves Alencar ◽  
Íthalo Barbosa Silva de Abreu ◽  
Emmanuel Damilano Dutra ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Closed Loop

scholarly journals Transforming biorefinery designs with ‘Plug-In Processes of Lignin’ to enable economic waste valorization

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhi-Hua Liu ◽  
Naijia Hao ◽  
Yun-Yan Wang ◽  
Chang Dou ◽  
Furong Lin ◽  
...  
Keyword(s):  
Cellulosic Ethanol ◽  
Cost Effective ◽  
Substantial Improvement ◽  
Selling Price ◽  
Waste Valorization ◽  
Lignin Valorization ◽  
Capital Costs ◽  
Carbon Efficiency ◽  
Synergistic Enhancement ◽  
Total Capital

AbstractBiological lignin valorization has emerged as a major solution for sustainable and cost-effective biorefineries. However, current biorefineries yield lignin with inadequate fractionation for bioconversion, yet substantial changes of these biorefinery designs to focus on lignin could jeopardize carbohydrate efficiency and increase capital costs. We resolve the dilemma by designing ‘plug-in processes of lignin’ with the integration of leading pretreatment technologies. Substantial improvement of lignin bioconversion and synergistic enhancement of carbohydrate processing are achieved by solubilizing lignin via lowering molecular weight and increasing hydrophilic groups, addressing the dilemma of lignin- or carbohydrate-first scenarios. The plug-in processes of lignin could enable minimum polyhydroxyalkanoate selling price at as low as $6.18/kg. The results highlight the potential to achieve commercial production of polyhydroxyalkanoates as a co-product of cellulosic ethanol. Here, we show that the plug-in processes of lignin could transform biorefinery design toward sustainability by promoting carbon efficiency and optimizing the total capital cost.

scholarly journals Engineered yeast genomes accurately assembled from pure and mixed samples

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Joseph H. Collins ◽  
Kevin W. Keating ◽  
Trent R. Jones ◽  
Shravani Balaji ◽  
Celeste B. Marsan ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Metagenomic Sequencing ◽  
Long Reads ◽  
Whole Genomes ◽  
Genome Features ◽  
Engineered Yeast ◽  
Yeast Genomes ◽  
Yeast Genetic ◽  
Yeast Plasmids ◽  
Assembly Software

AbstractYeast whole genome sequencing (WGS) lacks end-to-end workflows that identify genetic engineering. Here we present Prymetime, a tool that assembles yeast plasmids and chromosomes and annotates genetic engineering sequences. It is a hybrid workflow—it uses short and long reads as inputs to perform separate linear and circular assembly steps. This structure is necessary to accurately resolve genetic engineering sequences in plasmids and the genome. We show this by assembling diverse engineered yeasts, in some cases revealing unintended deletions and integrations. Furthermore, the resulting whole genomes are high quality, although the underlying assembly software does not consistently resolve highly repetitive genome features. Finally, we assemble plasmids and genome integrations from metagenomic sequencing, even with 1 engineered cell in 1000. This work is a blueprint for building WGS workflows and establishes WGS-based identification of yeast genetic engineering.

Enhancing cellulosic ethanol production through coevolution of multiple enzymatic characteristics of β-glucosidase from Penicillium oxalicum 16

2020 ◽  
Vol 104 (19) ◽  
pp. 8299-8308
Author(s):  
Qiuxia Huang ◽  
Kexin Wang ◽  
Hanxin Li ◽  
Shi Yi ◽  
Xihua Zhao
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Penicillium Oxalicum ◽  
Enzymatic Characteristics

Cellulosic ethanol: M&G and Beta Renewables to build unit in Slovakia

2014 ◽  
Vol 2014 (12) ◽  
pp. 5
Keyword(s):  
Cellulosic Ethanol
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