extracellular potassium
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2022 ◽  
Vol 12 ◽  
Author(s):  
Gareth Morris ◽  
Mona Heiland ◽  
Kai Lamottke ◽  
Haifeng Guan ◽  
Thomas D. M. Hill ◽  
...  

Drug-resistant epilepsy remains a significant clinical and societal burden, with one third of people with epilepsy continuing to experience seizures despite the availability of around 30 anti-seizure drugs (ASDs). Further, ASDs often have substantial adverse effects, including impacts on learning and memory. Therefore, it is important to develop new ASDs, which may be more potent or better tolerated. Here, we report the preliminary preclinical evaluation of BICS01, a synthetic product based on a natural compound, as a potential ASD. To model seizure-like activity in vitro, we prepared hippocampal slices from adult male Sprague Dawley rats, and elicited epileptiform bursting using high extracellular potassium. BICS01 (200 μM) rapidly and reversibly reduced the frequency of epileptiform bursting but did not change broad measures of network excitability or affect short-term synaptic facilitation. BICS01 was well tolerated following systemic injection at up to 1,000 mg/kg. However, we did not observe any protective effect of systemic BICS01 injection against acute seizures evoked by pentylenetetrazol. These results indicate that BICS01 is able to acutely reduce epileptiform activity in hippocampal networks. Further preclinical development studies to enhance pharmacokinetics and accumulation in the brain, as well as studies to understand the mechanism of action, are now required.


2022 ◽  
Author(s):  
Yangyang Yu ◽  
Jiajia Li ◽  
Zhixuan Yuan ◽  
Yongchen Fan ◽  
Ying Wu

Abstract Epilepsy is the second largest neurological disease which seriously threatens human life and health. The one important reason of inducing epilepsy is ischemic stroke which causes insufficient oxygen supply from blood vessels to neurons. However, few studies focus on the underlying mechanism of the generation and propagation of epilepsy after ischemic stroke by utilizing modeling methods. To explore the mechanism, this paper establishes a coupled network model consisting of neurons and astrocytes, and introduces a blood vessel to simulate the condition of ischemic stroke. First we study the effect of the degree of vascular blockage on the generation of epilepsy. The results demonstrate that the important reason of epilepsy after ischemic stroke is the disruption of ion concentration gradient. Then we study three factors that influence the epileptic propagation after ischemic stroke: massive glutamate release, excessive receptor activation and high extracellular potassium concentration. The results demonstrate that massive glutamate acting on postsynaptic neurons and the excessive activation of glutamate receptors on postsynaptic neurons promote the epileptic propagation in neuronal population, and massive glutamate acting on astrocytes and excessive activation of metabotropic glutamate receptors on presynaptic neurons inhibit the epileptic propagation, and the potassium uptake by astrocytes suppresses the epileptic propagation. The results are consistent with the experimental phenomena. The simulation results also shed light on the fact that astrocytes have neuroprotective effect. Our results on the generation and propagation of epilepsy after ischemic stroke could offer theoretical guidelines for the treatment of epilepsy after ischemic stroke.


2021 ◽  
Author(s):  
Ryan S Phillips ◽  
Jonathan E Rubin

Inspiratory breathing rhythms arise from synchronized neuronal activity in a bilaterally distributed brainstem structure known as the preBötzinger complex (preBötC). In in vitro slice preparations containing the preBötC, extracellular potassium must be elevated above physiological levels (to 7-9mM) to observe regular rhythmic respiratory motor output in the hypoglossal nerve to which the preBötC projects. Reexamination of how extracellular K+ affects preBötC neuronal activity has revealed that low amplitude oscillations persist at physiological levels. These oscillatory events are sub-threshold from the standpoint of transmission to motor output and are dubbed burstlets. Burstlets arise from synchronized neural activity in a rhythmogenic neuronal subpopulation within the preBötC that in some instances may fail to recruit the larger network events, or bursts, required to generate motor output. The fraction of subthreshold preBötC oscillatory events (burstlet fraction) decreases sigmoidally with increasing extracellular potassium. These observations underlie the burstlet theory of respiratory rhythm generation. Experimental and computational studies have suggested that recruitment of the non-rhythmogenic component of the preBötC population requires intracellular Ca2+ dynamics and activation of a calcium-activated non-selective cationic current. In this computational study, we show how intracellular calcium dynamics driven by synaptically triggered Ca2+ influx as well as Ca2+ release/uptake by the endoplasmic reticulum in conjunction with a calcium-activated non-selective cationic current can explain all of the key observations underlying the burstlet theory of respiratory rhythm generation. Thus, we provide a mechanistic basis to unify the experimental findings on rhythm generation and motor output recruitment in the preBötC.


Author(s):  
Haruhi Terai ◽  
Mayeso Naomi Victoria Gwedela ◽  
Koichi Kawakami ◽  
Hidenori Aizawa

Spreading depolarization (SD) is a slowly propagating wave of neuronal and glial depolarization. A growing number of studies show that SD and SD-like phenomena play a role in neurological disorders such as migraine, stroke, and traumatic brain injury. Despite the clinical importance of SD, its underlying molecular and cellular mechanisms remain elusive, possibly because of insufficient animal model allowing genetic manipulation. Such a model would also allow high-throughput screening for SD-suppressing drug development. To address this, we developed a novel experimental system to study SD using zebrafish. Electrophysiological recordings in the immobilized adult zebrafish revealed that increasing extracellular potassium concentration elicited SD with a large and long-lasting negative shift of direct current (DC) potential in the optic tectum. It also reduced the oscillatory activity in the extracellular field potential and increased the expression of the immediate early gene c-fos. Pharmacological blocking of the N-methyl-d-aspartate (NMDA) glutamate receptor attenuated the propagation of SD, suggesting that glutamatergic neurotransmission mediated tectal SD in zebrafish. Our analyses revealed that the zebrafish tectum and rodent cortex had similar SD kinetics. The current study provides electrophysiological and pharmacological evidence that zebrafish SD and mammal SD are comparable. This zebrafish SD model is suitable for genetic manipulation and cost-effective high-throughput screening. It could pave the way to novel diagnostic and therapeutic methods applicable to SD-associated neurological disorders.


2021 ◽  
Author(s):  
Wan-Yu Huang ◽  
Yen-Ling Lai ◽  
Ko-Hung Liu ◽  
Shankung Lin ◽  
Hsuan-Ying Chen ◽  
...  

Abstract BackgroundSystemic inflammation is a potent contributor to increased seizure susceptibility. However, less is known about the effects of systemic inflammation on blood-brain barrier (BBB) that affect neuron excitability. Necroptosis and inflammation are intimately associated in various neurological diseases. We hypothesized that necroptosis is involved in the mechanism underlying sepsis-associated neuronal excitability in BBB components.MethodsSystemic inflammation was induced by LPS. Seizure susceptibility of mice was measured by kainic acid intraperitoneal injection. Pharmacological inhibitors (C87 and GSK872) were used to block signaling of TNFα receptors and necroptosis. To identify the features of sepsis-associated response in the BBB and CNS, brain tissues of mice were obtained for assays of the necroptosis-related protein expression, and immunofluorescence staining for morphological changes of endothelia and glia. Microdialysis assay was also used to evaluate the changes of extracellular potassium and glutamate levels in brain.ResultsSignificant findings including induced increased seizure susceptibility and BBB endothelia necroptosis and leakage, Kir4.1 dysfunction, and microglia activation were observed in mice following LPS injection. Inhibition of TNFa receptor inhibitor C87 significantly attenuated increased kainic acid-induced seizure susceptibility and endothelia necroptosis and microglia activation, and restored kir4.1 protein expression, compared with those in controls. GSK872 (a RIP3 inhibitor) treatment, like C87, had consistent effects on these changes following LPS.ConclusionsOur results showed that TNFα-mediated necroptosis in BBB endothelia damage contributes to the development of increased seizure susceptibility in mice after systemic inflammation. Pharmacologic inhibition targeting this necroptosis pathway may provide a promising therapeutic approach to reduce sepsis-associated BBB dysfunction, astrocyte ion channel dysfunction, and subsequent neuronal excitability.


2021 ◽  
Vol 22 (19) ◽  
pp. 10312
Author(s):  
Takuma Kumamoto ◽  
Tomokazu Tsurugizawa

Astrocytes provide trophic and metabolic support to neurons and modulate circuit formation during development. In addition, astrocytes help maintain neuronal homeostasis through neurovascular coupling, blood–brain barrier maintenance, clearance of metabolites and nonfunctional proteins via the glymphatic system, extracellular potassium buffering, and regulation of synaptic activity. Thus, astrocyte dysfunction may contribute to a myriad of neurological disorders. Indeed, astrocyte dysfunction during development has been implicated in Rett disease, Alexander’s disease, epilepsy, and autism, among other disorders. Numerous disease model mice have been established to investigate these diseases, but important preclinical findings on etiology and pathophysiology have not translated into clinical interventions. A multidisciplinary approach is required to elucidate the mechanism of these diseases because astrocyte dysfunction can result in altered neuronal connectivity, morphology, and activity. Recent progress in neuroimaging techniques has enabled noninvasive investigations of brain structure and function at multiple spatiotemporal scales, and these technologies are expected to facilitate the translation of preclinical findings to clinical studies and ultimately to clinical trials. Here, we review recent progress on astrocyte contributions to neurodevelopmental and neuropsychiatric disorders revealed using novel imaging techniques, from microscopy scale to mesoscopic scale.


2021 ◽  
Vol 5 (1) ◽  
pp. 693-703
Author(s):  
Md A. Hakim ◽  
Erik J. Behringer

Background: As the sixth-leading cause of death in the United States, Alzheimer’s disease (AD) entails deteriorating endothelial control of blood flow throughout the brain. In particular, reduced inward-rectifying K+ (KIR) channel function in animal models of aging and AD compromises endothelial function and optimal perfusion of brain parenchyma. Deficient endothelial KIR channels may result from aberrant interaction with plasma membrane cholesterol as a primary regulator of membrane fluidity and ion channels. Objective: We tested the hypothesis that mild methyl-β-cyclodextrin (MβCD) treatment to reduce membrane cholesterol may restore endothelial KIR channel function in brain endothelium of old AD mice. Methods: Membrane potential was continuously measured in isolated endothelial tubes from posterior cerebral arteries of young (1 to 3 months) and old (16 to 19 months) female 3xTg-AD mice before and after mild treatment with the cholesterol-removing agent MβCD (1 mmol/L). Elevated extracellular potassium ([K+]E; 15 mmol/L) and NS309 (1μmol/L) activated KIR and Ca2+-activated K+ (SKCa/IKCa) channels respectively before and after MβCD treatment. Results: SKCa/IKCa channel function for producing hyperpolarization remained stable regardless of age group and MβCD treatment (ΔVm: ∼–33 mV). However, as deficient during AD, KIR channel function was restored (ΔVm: –9±1 mV) versus pre-MβCD conditions (–5±1 mV); a progressive effect that reached –14±1 mV hyperpolarization at 60 min following MβCD washout. Conclusion: In female animals, MβCD treatment of brain endothelium selectively restores KIR versus SKCa/IKCa channel function during AD. Thus, the endothelial cholesterol-KIR channel interface is a novel target for ameliorating perfusion of the AD brain.


2021 ◽  
Vol 22 (16) ◽  
pp. 8658
Author(s):  
Azin EbrahimAmini ◽  
Shanthini Mylvaganam ◽  
Paolo Bazzigaluppi ◽  
Mohamad Khazaei ◽  
Alexander Velumian ◽  
...  

A normally functioning nervous system requires normal extracellular potassium ion concentration ([K]o). Throughout the nervous system, several processes, including those of an astrocytic nature, are involved in [K]o regulation. In this study we investigated the effect of astrocytic photostimulation on [K]o. We hypothesized that in vivo photostimulation of eNpHR-expressing astrocytes leads to a decreased [K]o. Using optogenetic and electrophysiological techniques we showed that stimulation of eNpHR-expressing astrocytes resulted in a significantly decreased resting [K]o and evoked K responses. The amplitude of the concomitant spreading depolarization-like events also decreased. Our results imply that astrocytic membrane potential modification could be a potential tool for adjusting the [K]o.


Author(s):  
Margrethe A. Boyd ◽  
Anna M. Davis ◽  
Nora R. Chambers ◽  
Peter Tran ◽  
Arthur Prindle ◽  
...  

2021 ◽  
Vol 17 (7) ◽  
pp. e1009239
Author(s):  
Louisiane Lemaire ◽  
Mathieu Desroches ◽  
Martin Krupa ◽  
Lara Pizzamiglio ◽  
Paolo Scalmani ◽  
...  

Loss of function mutations of SCN1A, the gene coding for the voltage-gated sodium channel NaV1.1, cause different types of epilepsy, whereas gain of function mutations cause sporadic and familial hemiplegic migraine type 3 (FHM-3). However, it is not clear yet how these opposite effects can induce paroxysmal pathological activities involving neuronal networks’ hyperexcitability that are specific of epilepsy (seizures) or migraine (cortical spreading depolarization, CSD). To better understand differential mechanisms leading to the initiation of these pathological activities, we used a two-neuron conductance-based model of interconnected GABAergic and pyramidal glutamatergic neurons, in which we incorporated ionic concentration dynamics in both neurons. We modeled FHM-3 mutations by increasing the persistent sodium current in the interneuron and epileptogenic mutations by decreasing the sodium conductance in the interneuron. Therefore, we studied both FHM-3 and epileptogenic mutations within the same framework, modifying only two parameters. In our model, the key effect of gain of function FHM-3 mutations is ion fluxes modification at each action potential (in particular the larger activation of voltage-gated potassium channels induced by the NaV1.1 gain of function), and the resulting CSD-triggering extracellular potassium accumulation, which is not caused only by modifications of firing frequency. Loss of function epileptogenic mutations, on the other hand, increase GABAergic neurons’ susceptibility to depolarization block, without major modifications of firing frequency before it. Our modeling results connect qualitatively to experimental data: potassium accumulation in the case of FHM-3 mutations and facilitated depolarization block of the GABAergic neuron in the case of epileptogenic mutations. Both these effects can lead to pyramidal neuron hyperexcitability, inducing in the migraine condition depolarization block of both the GABAergic and the pyramidal neuron. Overall, our findings suggest different mechanisms of network hyperexcitability for migraine and epileptogenic NaV1.1 mutations, implying that the modifications of firing frequency may not be the only relevant pathological mechanism.


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