Biologically rapid synthesized silver nanoparticles from aqueous Eucalyptus camaldulensis leaf extract: Effects on hyphal growth, hydrolytic enzymes, and biofilm formation in Candida albicans

2021 ◽  
Author(s):  
Suttiwan Wunnoo ◽  
Supakit Paosen ◽  
Sakkarin Lethongkam ◽  
Rattanavadee Sukkurd ◽  
Tassanai Waen‐ngoen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Leaf Extract ◽  
Hydrolytic Enzymes ◽  
Eucalyptus Camaldulensis ◽  
Hyphal Growth

scholarly journals Facile and Eco-Friendly Fabrication of Silver Nanoparticles Using Nyctanthes arbor-tristis Leaf Extract to Study Antibiofilm and Anticancer Properties against Candida albicans

2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Beema Shafreen Rajamohamed ◽  
Seema Siddharthan ◽  
Velmurugan Palanivel ◽  
Mohanavel Vinayagam ◽  
Vijayanand Selvaraj ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Leaf Extract ◽  
Morphological Changes ◽  
Spherical Shape ◽  
Toxic Chemicals ◽  
X Ray ◽  
Anticancer Properties ◽  
Color Visualization

The synthesis of silver nanoparticles has been gaining more attention in recent years due to their small size and high stability. For this study, silver nanoparticles were biosynthesized from leaf extract of the medicinal plant (N. arbor-tristis). Vitally, the shrub with tremendous medicinal usage was diversely observed in South Asia and South East Asia. The synthesized silver nanoparticles were characterized by color visualization, ultraviolet-visible spectrophotometry (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), field emission-scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS) technique. A sharp peak at 427 nm for biosynthesized nanoparticles was obtained using UV-Vis, which represents surface plasmon resonance. Thus, characterization techniques showed the green synthesis of AgNPs leads to the fabrication of spherical shape particles with a size of 67 nm. Furthermore, AgNPs were subjected to antibiofilm studies against Candida albicans and it was observed that 0.5 μg mL−1 of AgNPs significantly reduced 50% of biofilm formation. These biosynthesized nanoparticles also showed a considerable reduction in viability of HeLa cells at 0.5 μg mL−1. The morphological changes induced by AgNPs were observed by AO/EB staining. The toxic effect of AgNPs was studied using brine shrimp as a model system. Therefore, it is envisaged that further investigation with these AgNPs can replace toxic chemicals, assist in the development of biomedical implants that can prevent biofilm formation, and avoid infections due to C. albicans.

scholarly journals CRISPR-Cas9 mutagenesis and single gene reintegration suggests functional diversity within the Candida albicans TLO gene family

2021 ◽  
Vol 3 (12) ◽  
Author(s):  
Jessica Fletcher ◽  
Gary Moran ◽  
Derek Sullivan
Keyword(s):  
Growth Rate ◽  
Candida Albicans ◽  
Gene Family ◽  
Biofilm Formation ◽  
Single Gene ◽  
Hyphal Growth ◽  
Detectable Effect ◽  
Null Mutant ◽  
Phenotypic Analysis ◽  
Functional Differences

Candida albicans has between 10-15 Telomere-associated ORF family(TLO)genes, whereas its closest relative, Candida dubliniensis, has two. The Tlo proteins are components of the Mediator complex which plays an important role in transcriptional regulation. CRISPR-Cas9 mutagenesis was used to generate a TLOnull mutant of C. albicans. Phenotypic analysis of the mutant showed significantly reduced fitness, with major defects in growth rate, morphogenesis, stress resistance and virulence in a Galleria mellonellamodel. Clade representative TLOα1, TLOβ2 and TLOγ11constructs were reintroduced into the null mutant background to determine if members of the TLO gene family exhibit functional differences. The genes were reintroduced under the control of the TET1 and ENO1promoters. TLOα1and TLOβ2expression restored stress tolerance and growth rate, in some cases to the level of the WT. TLOβ2expression also showed a dramatic effect on morphology resulting in constitutive true hyphal growth. Moderate expression of TLOγ11 had no detectable effect on many of the phenotypes tested, however overexpression increased biofilm formation in Spider medium, and also conferred increased resistance to cell wall stressors. These data suggest that individual TLO genes have distinct functions and that the diversity within the TLO family may contribute to the relative success of C. albicans as a coloniser and pathogen of humans.

Zerumbone inhibits Candida albicans biofilm formation and hyphal growth

2019 ◽  
Vol 65 (10) ◽  
pp. 713-721 ◽  
Author(s):  
Da-Seul Shin ◽  
Yong-Bin Eom
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Antifungal Agents ◽  
Hyphal Growth ◽  
Antibiofilm Activity ◽  
Potential Candidate ◽  
Zingiber Zerumbet ◽  
Dna Contents ◽  
Important Virulence Factor ◽  
Morphologic Changes

Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a monocyclic sesquiterpene extracted from Zingiber zerumbet (L.) Smith, against C. albicans biofilm formation. Our results suggest that zerumbone possesses antifungal and antibiofilm activity that inhibits biofilm formation and eradicates preformed biofilm. Notably, zerumbone considerably reduced carbohydrate and DNA contents of biofilm matrix. In addition, zerumbone showed antivirulence effects by decreasing the growth of hyphae and inhibiting morphologic changes of C. albicans. Furthermore, zerumbone significantly downregulated expression levels of biofilm-related and hyphae-specific genes, including HWP1 and ALS3. Since zerumbone suppresses biofilm formation and hyphae growth, these results indicate that zerumbone could be used as a potential candidate to treat and prevent C. albicans biofilm-related infections.

scholarly journals Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles

2012 ◽  
Vol 112 (6) ◽  
pp. 1163-1172 ◽  
Author(s):  
A.F. Wady ◽  
A.L. Machado ◽  
V. Zucolotto ◽  
C.A. Zamperini ◽  
E. Berni ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Acrylic Resin ◽  
Denture Base

Inhibitory effects of the essential oils α-longipinene and linalool on biofilm formation and hyphal growth of Candida albicans

Biofouling ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 143-155 ◽  
Author(s):  
Ranjith Kumar Manoharan ◽  
Jin-Hyung Lee ◽  
Yong-Guy Kim ◽  
Soon-Il Kim ◽  
Jintae Lee
Keyword(s):  
Candida Albicans ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Hyphal Growth ◽  
Inhibitory Effects

Penicillenols from a deep-sea fungus Aspergillus restrictus inhibit Candida albicans biofilm formation and hyphal growth

2017 ◽  
Vol 70 (6) ◽  
pp. 763-770 ◽  
Author(s):  
Jie Wang ◽  
Qi-Feng Yao ◽  
Muhammad Amin ◽  
Xu-Hua Nong ◽  
Xiao-Yong Zhang ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Deep Sea ◽  
Biofilm Formation ◽  
Hyphal Growth

Green Synthesis of Silver Nanoparticles Using Aqueous Extract of Lamium album and their Antifungal Properties

2021 ◽  
Vol 67 ◽  
pp. 55-67
Author(s):  
Zahra Zareshahrabadi ◽  
Fatemeh Karami ◽  
Saeed Taghizadeh ◽  
Aida Iraji ◽  
Ali Mohammad Amani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Biofilm Formation ◽  
Dependent Manner ◽  
Face Centered Cubic ◽  
Antifungal Activities ◽  
Lamium Album

Biosynthesis of novel metal nanoparticles (especially silver) using plant derivatives has received increasing attention due to their eco-friendly and potential applications in pharmaceutical and medical fields. In this study, silver nanoparticles were synthesized by using aqueous extract of Lamium album as a biocompatible and green method. The synthesized silver nanoparticles were characterized by Ultraviolet-visible (UV–vis) Spectroscopy, Transmission Electron Microscopy (TEM), X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), and Dynamic Light Scattering (DLS) Analysis. Inhibitory activity of silver nanoparticles on fungal growth was evaluated and inhibition of Candida albicans biofilms formation was measured using XTT assay. Their antioxidant and cytotoxic properties have also been evaluated.The synthesized AgNPs were mostly spherical in shape with an average size of nearly 25.2 nm. The AgNPs were crystalline in nature and have a face-centered cubic structure. The results of zeta potential value for AgNPs were −30 mV indicating the normal stability of the synthesized nanoparticles in colloidal systems. The result of antifungal activities showed that the biosynthesized silver nanoparticles had inhibitory activity against Candida albicans, C. tropicalis, C. krusei, C. glabrata, C. dubliniensis, C. parapsilosis, Cryptococcus neoformance, Aspergillus flavus, A. clavatus, Aspergillus fumigatus, Pseudallescheria boydii and Exophiala dermatitidis. The nanoparticles inhibited the Candida albicans biofilm formation in a dose-dependent manner. The cytotoxicity study of silver nanoparticles was revealed IC50 of 110.75 μg/mL against liver cell lines. The recognized bioactivity confirmed by the synthesized silver nanoparticles directs towards the potential for using as an antioxidant, antifungal, and cytotoxic agent.Keywords: Green synthesis, Antifungal activities, Silver nanoparticles, Lamium album, Biofilm formation.

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