Simple and rapid system for two-dimensional gel electrophoresis technique: A laboratory exercise for high school students

2018 ◽  
Vol 46 (3) ◽  
pp. 237-244 ◽  
Author(s):  
Praveen Maurye ◽  
Arpita Basu ◽  
Jayanta Kumar Biswas ◽  
Tapas Kumar Bandyopadhyay ◽  
Malay Naskar
Keyword(s):  
High School ◽  
High School Students ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
School Students ◽  
Two Dimensional Gel Electrophoresis ◽  
Laboratory Exercise ◽  
Electrophoresis Technique

User-friendly tool kits for protein gel electrophoresis techniques: A training program for high school students

2018 ◽  
Vol 46 (5) ◽  
pp. 566-577 ◽  
Author(s):  
Praveen Maurye ◽  
Arpita Basu ◽  
Sohini Sen ◽  
Jayanta Kumar Biswas ◽  
Tapas Kumar Bandyopadhyay ◽  
...  
Keyword(s):  
High School ◽  
High School Students ◽  
Training Program ◽  
Gel Electrophoresis ◽  
School Students ◽  
Protein Gel ◽  
User Friendly

scholarly journals Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis.

1977 ◽  
Vol 146 (5) ◽  
pp. 1261-1279 ◽  
Author(s):  
P P Jones
Keyword(s):  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Surface Proteins ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Cell Surface Proteins ◽  
Ia Antigens ◽  
Polypeptide Chains ◽  
Kinetics Of ◽  
Electrophoresis Technique

Mouse lymphocyte H-2 and Ia glycoproteins have been analyzed with a two-dimensional (2-D) acrylamide gel electrophoresis technique, in which proteins are separated first according to their charge in isoelectrofocusing gels and then according to their size in sodium dodecyl sulfate gels. Individual polypeptide chains from radiolabeled cells are resolved as discrete spots on autoradiograms of the gels, forming patterns which are characteristic of the proteins in the sample. 2-D gels of H-2K, H-2D, and Ia glycoproteins immunoprecipitated from 35S-methionine-labeled cells reveal that these proteins exist in the cells as complex arrays of molecules heterogeneous in both size and charge. Lactoperoxidase-catalyzed radioiodination of lymphocyte surfaces labels only subsets of the total H-2 and Ia molecules with 125I, indicating that some of the molecules may represent cytoplasmic precursors of the cell surface proteins. This theory is supported by the kinetics of labeling of various spots in 35S-methionine pulse-chase experiments. The 2-D gel patterns obtained for both H-2 and Ia antigens have also been shown to be haplotype-specific and independent of the genetic background.

Proteomics in the Classroom: An Investigative Study of Proteins in Microorganisms

2012 ◽  
Vol 74 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Jon Benskin ◽  
Sixue Chen
Keyword(s):  
Mass Spectrometry ◽  
High School ◽  
High School Students ◽  
Data Analysis ◽  
Molecular Biology ◽  
Gel Electrophoresis ◽  
Protein Extraction ◽  
Molecular Techniques ◽  
School Students

As advances in biotechnology and molecular biology rapidly expand in research settings, it is vital that we continue to prepare high school students to enter and thrive in those modern laboratories. This multistep, inquiry-based lab describes highly adaptable methods to teach students not only current molecular techniques and technologies, but also about proteomics and microorganisms. Students participate in protein extraction, gel electrophoresis, mass spectrometry, and data analysis to identify proteins present in microorganisms.

scholarly journals Analisis Kemampuan Representasi Siswa Dalam Menyelesaikan Soal Materi Bangun Datar

2021 ◽  
Vol 9 (2) ◽  
pp. 143-152
Author(s):  
Nabyllah Agnielia Mulyadi ◽  
Shofan Fiangga
Keyword(s):  
High School ◽  
Qualitative Research ◽  
High School Students ◽  
Junior High School ◽  
Junior High ◽  
Visual Representation ◽  
Junior High School Students ◽  
Two Dimensional ◽  
Research Subjects ◽  
School Students

Abstract Representation is the embodiment of a student's idea of a problem, and representation is used to assist students in solving the problem. This research is qualitative research that aims to analyze students' representational abilities in solving problems of a two-dimensional figure. The research instrument consisted of test questions with two-dimensional figure material and interviews. The research subjects were 2 junior high school students in Surabaya City who had different representational abilities. The results showed that students had moderate representational abilities because they gave rise to 2 indicators of representational ability, namely indicators of verbal and symbolic representation in different ways of conveying, but the two subjects had not yet produced indicators of visual representation. Keywords: Representation ability, junior high school, two-dimensional figure   Abstrak Representasi adalah perwujudan ide dari seorang siswa terhadap suatu masalah, dan representasi digunakan untuk membantu siswa dalam menyelesaikan masalah tersebut. Penelitian ini merupakan penelitian deskriptif kualitatif yang bertujuan untuk menganalisis kemampuan representasi siswa dalam menyelesaikan soal bangun datar. Instrumen penelitian terdiri dari soal tes dengan materi bangun datar dan pedoman wawancara. Subjek penelitian adalah 2 siswa SMP Kota Surabaya yang memiliki kemampuan representasi berbeda, subjek AM memenuhi indikator representasi A1, C1 dan subjek MIS memenuhi indikator representasi A1, C1, A2, C2. Hasil penelitian menunjukkan bahwa siswa memiliki kemampuan representasi sedang, karena memunculkan 2 tipe kemampuan representasi yaitu tipe representasi verbal dan simbolik dengan cara penyampaian yang berbeda, namun kedua subjek belum memunculkan tipe representasi visual. Kata Kunci: Kemampuan representasi, siswa SMP, bangun datar

scholarly journals Differences in Reading Strategies Employed by Students Constructing Graphic Organizers and Students Producing Summaries in EFL Reading

2006 ◽  
Vol 28 (2) ◽  
pp. 177 ◽  
Author(s):  
Akio Suzuki
Keyword(s):  
High School ◽  
High School Students ◽  
Reading Strategies ◽  
Graphic Organizers ◽  
Protocol Analysis ◽  
Comprehension Strategies ◽  
Two Dimensional ◽  
School Students ◽  
Visual Argument ◽  
Think Aloud Protocol

The big difference between summaries and graphic organizers (GOs) as adjunct aids for EFL reading is that GOs can reduce the cognitive burden on readers because of their two-dimensional spatial displays while summaries cannot. In this study, five Japanese high school students were required to construct GOs and another five were required to produce summaries while reading a passage written in English. They were required to report what they were thinking while reading the passage and producing adjunct aids so that their use of reading strategies could be examined. The results gained from think-aloud protocol analysis indicated that the GO group reported more general comprehension strategies than the summary group. The findings are discussed from the perspective that the task of constructing the GOs using visual argument allowed the students to employ more general comprehension strategies. 学習者の英文読解を補助するために、要約を書かせる方法と英文の構成を図式化させる方法（Graphic　Organizer；GO）がある。両者の最も大きな違いはGOの場合、二次元の空間配置を利用することによって読み手の認知的負荷を軽減できることにある。本研究では５名の被験者に構成図を、５名の被験者に要約を書かせる課題を与え、使用される読解方略の違いをプロトコル分析によって検討した。分散分析の結果、構成図を書いた群は、要約を書いた群よりも、テキスト全体の構成に関わる読解方略を多く使用し、言語的な情報に関わる読解方略を少なくすることが示された。図式化した読者は情報を視覚化することにより、より包括的な読解方略を使ったためであろうという解釈がなされた。

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