Differentiation between isomeric oxidative metabolites of a piperidine-containing drug by liquid chromatography-tandem mass spectrometry with stable-isotope tracer technique

2002 ◽  
Vol 16 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Koji Kato ◽  
Shigeji Jingu ◽  
Naoyoshi Ogawa ◽  
Shohei Higuchi
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass ◽  
Tracer Technique ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Chromatography Tandem Mass Spectrometry ◽  
Oxidative Metabolites ◽  
Liquid Chromatography Tandem Mass

Stable isotope-dilution liquid chromatography/tandem mass spectrometry method for determination of thyroxine in saliva

2011 ◽  
Vol 879 (13-14) ◽  
pp. 1013-1017 ◽  
Author(s):  
Tatsuya Higashi ◽  
Takuya Ichikawa ◽  
Chikara Shimizu ◽  
So Nagai ◽  
Shinsuke Inagaki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Spectrometry Method ◽  
Stable Isotope Dilution ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Micromethod for Quantification of Cinacalcet in Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry Using a Stable Isotope-Labeled Internal Standard

2013 ◽  
Vol 35 (1) ◽  
pp. 112-117 ◽  
Author(s):  
Giuliana Cangemi ◽  
Sebastiano Barco ◽  
Enrico E. Verrina ◽  
Samuele Scurati ◽  
Giovanni Melioli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
Human Plasma ◽  
Internal Standard ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A sensitive method to determine melatonin in saliva by automated online in-tube solid-phase microextraction coupled with stable isotope-dilution liquid chromatography-tandem mass spectrometry

2017 ◽  
Vol 9 (21) ◽  
pp. 3134-3140 ◽  
Author(s):  
Atsushi Ishizaki ◽  
Akiko Uemura ◽  
Hiroyuki Kataoka
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Tandem Mass ◽  
Stable Isotope Dilution ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Sensitive Method

Melatonin (MLT) plays important roles in regulating the sleep-wake cycle, and has many beneficial effects on health. A simple, rapid, and sensitive method was developed for the determination of MLT in human saliva by automated online in-tube solid-phase microextraction coupled with stable isotope-dilution liquid chromatography-tandem mass spectrometry.

scholarly journals Measurement of Urinary d- and l-2-Hydroxyglutarate Enantiomers by Stable-Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry after Derivatization with Diacetyl-l-Tartaric Anhydride

2004 ◽  
Vol 50 (8) ◽  
pp. 1391-1395 ◽  
Author(s):  
Eduard A Struys ◽  
Erwin E W Jansen ◽  
Nanda M Verhoeven ◽  
Cornelis Jakobs
Keyword(s):  
Mass Spectrometry ◽  
Differential Diagnosis ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Internal Standards ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Abstract Background: The differential diagnosis of d-2-hydroxyglutaric aciduria (d-2-HGA), l-2-hydroxyglutaric aciduria (l-2-HGA), and the combined d/l-2-hydroxyglutaric aciduria (d/l-2-HGA) can be accomplished only by the measurement of the corresponding 2-hydroxyglutarate (2-HG). Available methods for the determination of d- and l-2-HG in urine are either time-consuming and expensive or have not been extensively validated. We aimed to develop a method for their rapid and sensitive measurement. Methods: We used liquid chromatography–tandem mass spectrometry (LC-MS/MS) for the determination of d- and l-2-HG with stable-isotope-labeled internal standards. Urine samples of 20 μL were mixed with 250 μL of methanol containing the internal standards and subsequently dried under nitrogen. The analytes were derivatized by use of diacetyl-l-tartaric anhydride (DATAN) to obtain diastereomers, which were separated on an achiral C18 HPLC column and detected by MS/MS in multiple-reaction-monitoring mode. Results: The use of DATAN as chiral derivatization reagent provided very well separated peaks of the formed diastereomers of d- and l-2-HG, with a total runtime of 5 min. The inter- and intraassay CVs for d- and l-2-HG ranged from 3.4% to 6.2%. Mean recoveries of d- and l-2-HG, evaluated on two concentrations, were 94%. Detection limit of the presented method was 20 pmol for a sample volume of 20 μL. Method comparison of the LC-MS/MS method with a gas chromatography–mass spectrometry method, in which d- and l-2-HG were derivatized with R-(−)-butanol, showed good agreement between the two methods. Conclusions: Urinary d- and l-2-HG can be analyzed by MS/MS after derivatization with DATAN. The presented method may be suitable for the differential diagnosis of 2-HGA.

Quantification of benzo[a]pyrene diol epoxide DNA-adducts by stable isotope dilution liquid chromatography/tandem mass spectrometry

2006 ◽  
Vol 20 (8) ◽  
pp. 1369-1380 ◽  
Author(s):  
Qian Ruan ◽  
Hye-Young H. Kim ◽  
Hao Jiang ◽  
Trevor M. Penning ◽  
Ronald G. Harvey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
Dna Adducts ◽  
Isotope Dilution ◽  
Tandem Mass ◽  
Stable Isotope Dilution ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass
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