Comparative expression analysis of hypoxia-inducible factor-alpha and its natural occurring antisense in breast cancer tissues and adjacent noncancerous tissues

2016 ◽  
Vol 34 (8) ◽  
pp. 572-578 ◽  
Author(s):  
Behnoosh Tasharrofi ◽  
Mohammad Soudyab ◽  
Elahe Nikpayam ◽  
Mostafa Iranpour ◽  
Reza Mirfakhraie ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Expression Analysis ◽  
Hypoxia Inducible Factor ◽  
Comparative Expression Analysis ◽  
Factor Alpha ◽  
Cancer Tissues

scholarly journals The G Protein-coupled Receptor 30 Is Up-regulated by Hypoxia-inducible Factor-1α (HIF-1α) in Breast Cancer Cells and Cardiomyocytes

2011 ◽  
Vol 286 (12) ◽  
pp. 10773-10782 ◽  
Author(s):  
Anna Grazia Recchia ◽  
Ernestina Marianna De Francesco ◽  
Adele Vivacqua ◽  
Diego Sisci ◽  
Maria Luisa Panno ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Hypoxia Inducible Factor ◽  
Low Oxygen ◽  
Downstream Target ◽  
Adaptive Mechanisms ◽  
Cancer Tissues ◽  
Apoptotic Effects ◽  
G Protein Coupled

GPR30, also known as GPER, has been suggested to mediate rapid effects induced by estrogens in diverse normal and cancer tissues. Hypoxia is a common feature of solid tumors involved in apoptosis, cell survival, and proliferation. The response to low oxygen environment is mainly mediated by the hypoxia-inducible factor named HIF-1α, which activates signaling pathways leading to adaptive mechanisms in tumor cells. Here, we demonstrate that the hypoxia induces HIF-1α expression, which in turn mediates the up-regulation of GPER and its downstream target CTGF in estrogen receptor-negative SkBr3 breast cancer cells and in HL-1 cardiomyocytes. Moreover, we show that HIF-1α-responsive elements located within the promoter region of GPER are involved in hypoxia-dependent transcription of GPER, which requires the ROS-induced activation of EGFR/ERK signaling in both SkBr3 and HL-1 and cells. Interestingly, the apoptotic response to hypoxia was prevented by estrogens through GPER in SkBr3 cells. Taken together, our data suggest that the hypoxia-induced expression of GPER may be included among the mechanisms involved in the anti-apoptotic effects elicited by estrogens, particularly in a low oxygen microenvironment.

Expression analysis of growth arrest specific 8 and its anti-sense in breast cancer tissues

2020 ◽  
Vol 114 ◽  
pp. 104414
Author(s):  
Sepideh Dashti ◽  
Zahra Taherian-Esfahani ◽  
Vahid Kholghi-Oskooei ◽  
Soudeh Ghafouri-Fard ◽  
Mohammad Taheri
Keyword(s):  
Breast Cancer ◽  
Expression Analysis ◽  
Growth Arrest ◽  
Cancer Tissues

scholarly journals PCAT-1 Facilitates Breast Cancer Progression via Enhancing Oxygen-independent Stability of Hypoxia-inducible Factor-1α

2020 ◽  
Author(s):  
Jianlong Wang ◽  
Xuyi Chen ◽  
Ning Zhang ◽  
Jianzhao Gao ◽  
Bin Liu
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Metabolic Regulation ◽  
Hypoxia Inducible Factor ◽  
Breast Cancer Progression ◽  
Physical Interaction ◽  
Breast Cancer Patients ◽  
Loss Of Function ◽  
Hypoxia Inducible Factor 1Α ◽  
Cancer Tissues

Abstract Background: Hypoxia induces a series of cellular adaptive responses that enable to promote inflammation and cancer development. However, only few have been fully characterized about the roles of long noncoding RNAs (lncRNAs) in hypoxia-associated cancer progression. Methods: The involvement of lncRNAs in hypoxia-related cancer progression was screened by qRT-PCR. Based on the public databases and integrating bioinformatics analysis, the alteration of prostate cancer associated transcript-1 (PCAT-1) in breast cancer tissues was detected and validated in a cohort of breast cancer tissues. Overexpression and knocking down experiments were performed to uncover the biological roles of PCAT-1 on cell hypoxia-associated phenotypes and biological behaviors. RNA immunoprecipitation (RIP) and RNA pull-down were carried out to reveal the physical interaction between PCAT-1 and receptor of activated protein-C kinase-1 (RACK1). Moreover, xenograft mouse models were used to evaluate the influence of PCAT-1 on cancer progression and metastasis in vivo.Results: We identified PCAT-1 as a hypoxia-inducible lncRNA that regulated the hypoxia-inducible factor-1α (HIF-1α) stability, crucial for cancer progression. Extensive analyses of clinical data indicated that PCAT-1 was elevated in breast cancer patients and was associated with pathological grade, tumor size and poor clinical outcomes. Through gain and loss of function experiments, we found that PCAT-1 promoted hypoxia-associated breast cancer progression including growth, migration, invasion, colony formation, and metabolic regulation. Mechanistically, PCAT-1 directly interacted with RACK1 protein and prevented RACK1 from binding to HIF-1α, thus protecting HIF-1α from RACK1-induced oxygen-independent degradation.Conclusions: These findings provide a new insight into lncRNA-mediated mechanisms for HIF-1α stability and suggest that a novel role of PCAT-1 as a potential therapeutic target for breast cancer.

scholarly journals Expression Analysis of MALAT1, GAS5, SRA, and NEAT1 lncRNAs in Breast Cancer Tissues from Young Women and Women over 45 Years of Age

2018 ◽  
Vol 12 ◽  
pp. 751-757 ◽  
Author(s):  
Asghar Arshi ◽  
Fatemeh Sadat Sharifi ◽  
Milad Khorramian Ghahfarokhi ◽  
Zahra Faghih ◽  
Abbas Doosti ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Expression Analysis ◽  
Young Women ◽  
Cancer Tissues

scholarly journals Expression Analysis of Two Cancer-testis Genes, FBXO39 and TDRD4, in Breast Cancer Tissues and Cell Lines

2013 ◽  
Vol 14 (11) ◽  
pp. 6625-6629 ◽  
Author(s):  
Mahnaz Seifi-Alan ◽  
Roshanak Shamsi ◽  
Soudeh Ghafouri-Fard ◽  
Reza Mirfakhraie ◽  
Davood Zare-Abdollahi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Expression Analysis ◽  
Cancer Tissues ◽  
Cancer Testis
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