scholarly journals Neonatal procedural pain affects state, but not trait anxiety behavior in adult rats

2021 ◽  
Vol 63 (8) ◽  
Author(s):  
Anne R de Kort ◽  
Elbert A Joosten ◽  
Jacob Patijn ◽  
Dick Tibboel ◽  
Nynke J van den Hoogen
Keyword(s):  
Trait Anxiety ◽  
Procedural Pain ◽  
Adult Rats ◽  
Anxiety Behavior

Anxiogenic-like profile of Wistar adult rats based on the pilocarpine model: an animal model for trait anxiety?

2012 ◽  
Vol 227 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Filipe S. Duarte ◽  
Marcelo Duzzioni ◽  
Alexandre A. Hoeller ◽  
Nayana M. Silva ◽  
Andy L. Ern ◽  
...  
Keyword(s):  
Animal Model ◽  
Trait Anxiety ◽  
Adult Rats ◽  
Pilocarpine Model

scholarly journals Relationship of Genetic Variants With Procedural Pain, Anxiety, and Distress in Children

2017 ◽  
Vol 19 (3) ◽  
pp. 339-349 ◽  
Author(s):  
Anne L. Ersig ◽  
Debra L. Schutte ◽  
Jennifer Standley ◽  
Elizabeth Leslie ◽  
Bridget Zimmerman ◽  
...  
Keyword(s):  
Trait Anxiety ◽  
Genomic Variation ◽  
Self Report ◽  
Procedural Pain ◽  
Candidate Gene Approach ◽  
Medical Procedure ◽  
Pain Anxiety ◽  
Biological Parents ◽  
Family Based ◽  
High Trait Anxiety

Objective: This study used a candidate gene approach to examine genomic variation associated with pain, anxiety, and distress in children undergoing a medical procedure. Study Design: Children aged 4–10 years having an IV catheter insertion were recruited from three Midwestern children’s hospitals. Self-report measures of pain, anxiety, and distress were obtained as well as an observed measure of distress. Samples were collected from children and biological parents for analysis of genomic variation. Genotyped variants had known or suspected association with phenotypes of interest. Analyses included child-only association and family-based transmission disequilibrium tests. Results: Genotype and phenotype data were available from 828 children and 376 family trios. Children were 50% male, had a mean age of 7.2 years, and were 84% White/non-Hispanic. In family-based analysis, one single-nucleotide polymorphism (SNP; rs1143629, interleukin ( IL1B) 1β) was associated with observed child distress at Bonferroni-corrected levels of significance ( p = .00013), while two approached significance for association with high state anxiety (rs6330 Nerve Growth Factor, Beta Subunit, [ NGFB]) and high trait anxiety (rs6265 brain-derived neurotrophic factor [ BDNF]). In the child-only analysis, multiple SNPs showed nominal evidence of relationships with phenotypes of interest. rs6265 BDNF and rs2941026 cholecystokinin B receptor had possible relationships with trait anxiety in child-only and family-based analyses. Conclusions: Exploring genomic variation furthers our understanding of pain, anxiety, and distress and facilitates genomic screening to identify children at high risk of procedural pain, anxiety, and distress. Combined with clinical observations and knowledge, such explorations could help guide tailoring of interventions to limit procedure-related distress and identify genes and pathways of interest for future genotype–phenotype studies.

Repeated forced swim stress has additive effects in anxiety behavior and in cathecolamine levels of adult rats exposed to deltamethrin

2014 ◽  
Vol 46 ◽  
pp. 57-61 ◽  
Author(s):  
Soraya F. Habr ◽  
Daclé J. Macrini ◽  
Helenice de S. Spinosa ◽  
Jorge C. Florio ◽  
Maria M. Bernardi
Keyword(s):  
Additive Effects ◽  
Swim Stress ◽  
Adult Rats ◽  
Forced Swim ◽  
Forced Swim Stress ◽  
Anxiety Behavior

scholarly journals Anxiety Influences Children’s Memory for Procedural Pain

2009 ◽  
Vol 14 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Elizabete M Rocha ◽  
Tammy A Marche ◽  
Carl L von Baeyer
Keyword(s):  
Pain Management ◽  
Trait Anxiety ◽  
Three Dimensions ◽  
Procedural Pain ◽  
Management Intervention ◽  
Dental Procedures ◽  
Pain Ratings ◽  
Children's Memory

OBJECTIVE: To examine the effects of temperament and trait anxiety on memory for pain.METHODS: Three dimensions of temperament, as well as trait anxiety, were assessed in 36 children (five to 12 years of age) undergoing dental procedures; after the procedure, the children provided pain ratings. Following a six- to eight-week delay, the children reported how much pain they remembered.RESULTS: Most children (85%) accurately recalled their pain. Temperament had no significant effect, but trait-anxious children showed a greater likelihood of recalling more pain than they initially reported, suggesting that they may negatively distort recollections of painful experiences.CONCLUSIONS: When treating children, in particular trait-anxious children, clinicians should consider what children remember as part of pain management intervention.

scholarly journals Prefrontal dopamine D1 receptor manipulation influences anxiety behavior and induces neuroinflammation within the hippocampus

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Dominik K. E. Beyer ◽  
Annika Mattukat ◽  
Nadja Freund
Keyword(s):  
Bipolar Disorder ◽  
Animal Model ◽  
Elevated Plus Maze ◽  
D1 Receptor ◽  
Adult Rats ◽  
Male Adult ◽  
Respective Control ◽  
Plus Maze ◽  
Inflammatory State ◽  
Anxiety Behavior

Abstract Background Prefrontal dopamine D1 receptor (D1R) mediates behavior related to anxiety, reward and memory, and is involved in inflammatory processes, all of which are affected in bipolar disorder. Interleukin-6 (IL-6), a pro-inflammatory cytokine, is increased in patients with bipolar disorder in plasma samples, imaging studies and postmortem tissue and is an indicator for an inflammatory state. We could previously show that lentiviral overexpression of D1R in the medial prefrontal cortex (mPFC) of male adult rats and its termination induces bipolar disorder-like behavior. The purpose of this study was to investigate anxiety and the role of the immune system, specifically IL-6 positive neurons in this animal model. Due to its high density of inflammatory mediator receptors and therewith sensibility to immune activation, the hippocampus was investigated. Methods Expression of the gene for D1R in glutamatergic neurons within the mPFC of male, adult rats was manipulated through an inducible lentiviral vector. Animals over-expressing the gene (mania-like state), after termination of the expression (depressive-like) and their respective control groups were investigated. Anxiety behavior was studied in the elevated plus maze and marble burying test. Furthermore, IL-6-positive cells were counted within several subregions of the hippocampus. Results D1R manipulation in the mPFC had only mild effects on anxiety behavior in the elevated plus maze. However, subjects after termination buried more marbles compared to D1R over-expressing animals and their respective control animals indicating elevated anxiety behavior. In addition, animals in the depressive-like state showed higher numbers of IL-6 positive cells reflecting an elevated pro-inflammatory state in the hippocampus, in the CA3 and dentate gyrus. Consistently, inflammatory state in the whole hippocampus and anxiety behavior correlated positively, indicating a connection between anxiety and inflammatory state of the hippocampus. Conclusions Behavioral and neurobiological findings support the association of manipulation of the D1R in the mPFC on anxiety and inflammation in the hippocampus. In addition, by confirming changes in the inflammatory state, the proposed animal model for bipolar disorder has been further validated.

Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

1974 ◽  
Vol 32 ◽  
pp. 288-289
Author(s):  
Alfredo Feria-Velasco ◽  
Guadalupe Tapia-Arizmendi
Keyword(s):  
Fine Structure ◽  
Domestic Fowl ◽  
Animal Species ◽  
Acinar Cells ◽  
Harderian Gland ◽  
Myoepithelial Cells ◽  
The Other ◽  
Albino Rats ◽  
Adult Rats ◽  
Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

1992 ◽  
Vol 50 (2) ◽  
pp. 1094-1095
Author(s):  
Beverly L. Giammara ◽  
Jennifer S. Stevenson ◽  
Peggy E. Yates ◽  
Robert H. Gunderson ◽  
Jacob S. Hanker
Keyword(s):  
Image Analysis ◽  
Basement Membrane ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Computer Assisted ◽  
Type Iii Collagen ◽  
Image Analysis System ◽  
Biodegradable Polyester ◽  
Adult Rats ◽  
Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

High-voltage EM description of the types and distribution of sensory endings in the inner conical body of the rat vibrissal follicle-sinus complex

1990 ◽  
Vol 48 (3) ◽  
pp. 410-411
Author(s):  
Tony M. Mosconi ◽  
Min J. Song ◽  
Frank L. Rice
Keyword(s):  
Mammalian Species ◽  
Dorsal Side ◽  
Hair Shaft ◽  
Sensory Innervation ◽  
Conical Body ◽  
Adult Rats ◽  
Perpendicular Plane ◽  
Ultrastructural Studies ◽  
Unmyelinated Axons ◽  
Sensory Endings

Whiskers or vibrissal follicle-sinus complexes (F-SCs) on the snouts of many mammalian species are structures that have complex, dense sensory innervation. The innervation of F-SCs is remarkably similar in all species with the exception of one site - the inner conical body (ICB). The ICB is an elongated cylindrical structure that encircles the hair shaft near the neck of the follicle. This site has received only cursory attention in ultrastructural studies of the F-SCAdult rats were perfused after the method of Renehan and Munger2. F-SCs were quartered longitudinally and embedded separately in Epon-Araldite. Serial 0.25 μm sections were cut in either the longitudinal or perpendicular plane through the ICB and examined with an AEI EM7 1.2 MV HVEM (Albany, NY) at 1000 KV. Sensory endings were reconstructed from serial micrographs through at least 20 μm in the longitudinal plane and through 10 μm in the perpendicular plane.From two to six small superficial vibrissal nerves converge upon the neck of the F-SC and descend into the ICB. The nerves branch into smaller bundles of myelinated and unmyelinated axons along the dorsal side of the hair shaft.

Effects of Fear Induction on Heart Period Variability

1999 ◽  
Vol 13 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Rudolf Stark ◽  
Alfons Hamm ◽  
Anne Schienle ◽  
Bertram Walter ◽  
Dieter Vaitl
Keyword(s):  
Blood Pressure ◽  
Trait Anxiety ◽  
State Anxiety ◽  
Self Report ◽  
Emotional States ◽  
Heart Period ◽  
Experimental Conditions ◽  
Heart Period Variability ◽  
Physiological Variables ◽  
Period Variability

Abstract The present study investigated the influence of contextual fear in comparison to relaxation on heart period variability (HPV), and analyzed differences in HPV between low and high anxious, nonclinical subjects. Fifty-three women participated in the study. Each subject underwent four experimental conditions (control, fear, relaxation, and a combined fear-relaxation condition), lasting 10 min each. Fear was provoked by an unpredictable aversive human scream. Relaxation should be induced with the aid of verbal instructions. To control for respiratory effects on HPV, breathing was paced at 0.2 Hz using an indirect light source. Besides physiological measures (HPV measures, ECG, respiration, forearm EMG, blood pressure), emotional states (pleasure, arousal, dominance, state anxiety) were assessed by subjects' self-reports. Since relaxation instructions did not have any effect neither on the subjective nor on the physiological variables, the present paper focuses on the comparison of the control and the fear condition. The scream reliably induced changes in both physiological and self-report measures. During the fear condition, subjects reported more arousal and state anxiety as well as less pleasure and dominance. Heart period decreased, while EMG and diastolic blood pressure showed a tendency to increase. HPV remained largely unaltered with the exception of the LF component, which slightly decreased under fear induction. Replicating previous findings, trait anxiety was negatively associated with HPV, but there were no treatment-specific differences between subjects with low and high trait anxiety.

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