Interleukin-6 treatment induces beta-cell apoptosis via STAT-3-mediated nitric oxide production

2011 ◽  
Vol 27 (8) ◽  
pp. 813-819 ◽  
Author(s):  
Yoon Sin Oh ◽  
Youn-Jung Lee ◽  
Eun Young Park ◽  
Hee-Sook Jun
Keyword(s):  
Nitric Oxide ◽  
Beta Cell ◽  
Interleukin 6 ◽  
Cell Apoptosis ◽  
Beta Cell Apoptosis ◽  
Nitric Oxide Production ◽  
Oxide Production

Coacervate microdroplet protocells-mediated gene transfection for nitric oxide production and induction of cell apoptosis

2021 ◽  
Author(s):  
Yan-Wen Zhang ◽  
Yu Yao ◽  
Songyang Liu ◽  
Yufeng Chen ◽  
Shaohong Zhou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Apoptosis ◽  
Gene Transfection ◽  
Nitric Oxide Production ◽  
Therapeutic Applications ◽  
Oxide Production ◽  
Gene Carriers ◽  
Cargo Delivery

Liquid coacervate microdroplets have been widely explored as membrane-free compartment protocells for cargo delivery in therapeutic applications. In this study, coacervate protocells were developed as gene carriers for transfection of...

scholarly journals MicroRNA-181c Inhibits Interleukin-6-mediated Beta Cell Apoptosis by Targeting TNF-α Expression

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1410 ◽  
Author(s):  
Yoon Sin Oh ◽  
Gong Deuk Bae ◽  
Eun-Young Park ◽  
Hee-Sook Jun
Keyword(s):  
Beta Cell ◽  
Mrna Expression ◽  
Beta Cells ◽  
Interleukin 6 ◽  
Cell Apoptosis ◽  
Neutralizing Antibodies ◽  
Annexin V ◽  
Beta Cell Apoptosis ◽  
Tnf Α ◽  
Long Term Treatment

We have previously reported that long-term treatment of beta cells with interleukin-6 (IL-6) is pro-apoptotic. However, little is known about the regulatory mechanisms that are involved. Therefore, we investigated pro-apoptotic changes in mRNA expression in beta cells in response to IL-6 treatment. We analyzed a microarray with RNA from INS-1 beta cells treated with IL-6, and found that TNF-α mRNA was significantly upregulated. Inhibition of TNF-α expression by neutralizing antibodies significantly decreased annexin V staining in cells compared with those treated with a control antibody. We identified three microRNAs that were differentially expressed in INS-1 cells incubated with IL-6. In particular, miR-181c was significantly downregulated in IL-6-treated cells compared with control cells and the decrease of miR-181c was attenuated by STAT-3 signaling inhibition. TNF-α mRNA was a direct target of miR-181c and upregulation of miR-181c by mimics, inhibited IL-6-induced increase in TNF-α mRNA expression. Consequently, reduction of TNF-α mRNA caused by miR-181c mimics enhanced cell viability in IL-6 treated INS-1 cells. These results demonstrated that miR-181c regulation of TNF-α expression plays a role in IL-6-induced beta cell apoptosis.

Nitric Oxide Production is not involved in the Effects of Interleukin-1β on CAMP, Thyroglobulin and Interleukin-6 in TSH-Stimulated Human Thyroid Cells

Autoimmunity ◽  
1994 ◽  
Vol 19 (4) ◽  
pp. 239-245 ◽  
Author(s):  
Ase K. Rasmussen ◽  
Roberto Di Marco ◽  
Marcus Diamant ◽  
Ulla Feldt-Rasmussen ◽  
Klaus Bendtzen
Keyword(s):  
Nitric Oxide ◽  
Interleukin 6 ◽  
Interleukin 1Β ◽  
Human Thyroid ◽  
Nitric Oxide Production ◽  
Thyroid Cells ◽  
Oxide Production

scholarly journals Effect of interleukin-6 and interleukin-10 on nitric oxide production by endothelial cells

Critical Care ◽  
10.1186/cc157 ◽  
1998 ◽  
Vol 2 (Suppl 1) ◽  
pp. P027
Author(s):  
V Brix-Christensen ◽  
Y Liu ◽  
E Tonnesen ◽  
G Stefano
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Interleukin 6 ◽  
Interleukin 10 ◽  
Nitric Oxide Production ◽  
Oxide Production
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