scholarly journals Do dried blood spots (DBS) have the potential to support result management processes in routine sports drug testing?

2020 ◽  
Vol 12 (6) ◽  
pp. 704-710 ◽  
Author(s):  
Mario Thevis ◽  
Tiia Kuuranne ◽  
Josef Dib ◽  
Andreas Thomas ◽  
Hans Geyer
Keyword(s):  
Drug Testing ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Sports Drug Testing ◽  
Management Processes ◽  
Result Management

scholarly journals Oral Testosterone Administration Detected by Testosterone Glucuronidation Measured in Blood Spots Dried on Filter Paper

2000 ◽  
Vol 46 (4) ◽  
pp. 515-522 ◽  
Author(s):  
Shi-Hua Peng ◽  
Jordi Segura ◽  
Magí Farré ◽  
Xavier de la Torre
Keyword(s):  
Filter Paper ◽  
Drug Testing ◽  
Dried Blood Spots ◽  
Gas Chromatography Mass Spectrometry ◽  
Specific Marker ◽  
Plasma Samples ◽  
Suitable Alternative ◽  
Testosterone Undecanoate ◽  
Blood Spots ◽  
Sports Drug Testing

Abstract Background: Blood sampling is not a common practice for sports drug testing. Our aim was to investigate whether dried blood spots on filter paper could be an alternative to plasma samples for monitoring steroid profiles in dope testing. Methods: We collected dried blood spots and plasma from six healthy Caucasian subjects after an oral 120-mg dose of testosterone undecanoate (TU). Nonconjugated testosterone, testosterone glucuronide (TG), androsterone glucuronide (AG), and etiocholanolone glucuronide (EtG) were measured by gas chromatography–mass spectrometry in both matrices. 17α-Hydroxyprogesterone (17αOHP) and luteinizing hormone (LH) also were measured in the plasma samples. For comparison, similar measurements were done on samples obtained from the same subjects given 25 mg of testosterone propionate (TP) plus 110 mg of testosterone enanthate (TE) intramuscularly after a wash-out period. Results: After oral TU intake, TG, AG, and EtG increased sharply, whereas nonconjugated testosterone did not change significantly. Results on dried blood spots correlated well with those on plasma. The TG/testosterone ratio in blood or plasma was verified to be a sensitive and specific marker (significantly increased for up to 8 h after intake; P <0.05) for oral TU intake but not for intramuscular administration of TP plus TE. Little suppression of plasma LH and 17αOHP was observed after a single oral dose of TU. One subject did not show a significant increase of blood TG after oral TU intake. Conclusions: The measurement of glucuronide conjugates in blood and plasma samples is relevant for sports drug testing when analyzing the steroid profile. Dried blood spots collected on filter paper are a suitable alternative to plasma for detecting testosterone abuse.

Development and validation of a mass spectrometric detection method of peginesatide in dried blood spots for sports drug testing

2012 ◽  
Vol 403 (9) ◽  
pp. 2715-2724 ◽  
Author(s):  
Ines Möller ◽  
Andreas Thomas ◽  
Hans Geyer ◽  
Wilhelm Schänzer ◽  
Mario Thevis
Keyword(s):  
Drug Testing ◽  
Detection Method ◽  
Dried Blood Spots ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Blood Spots ◽  
Sports Drug Testing ◽  
Development And Validation

Dried blood spots (DBS) in doping controls: a complementary matrix for improved in- and out-of-competition sports drug testing strategies

2015 ◽  
Vol 7 (18) ◽  
pp. 7596-7605 ◽  
Author(s):  
Laura Tretzel ◽  
Andreas Thomas ◽  
Hans Geyer ◽  
Valentin Pop ◽  
Wilhelm Schänzer ◽  
...  
Keyword(s):  
Cost Efficiency ◽  
Drug Testing ◽  
State Of The Art ◽  
Analytical Techniques ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Testing Strategies ◽  
In Doping ◽  
Efficiency Test

The use of dried blood spots as a complementary sample matrix combined with state-of-the-art analytical techniques substantially improves doping control efforts, particularly concerning cost-efficiency, test frequency, and sample collection invasiveness.

Application of oral fluid and dried blood spots as a matrix for roadside drug testing

2015 ◽  
pp. 94-109 ◽  
Author(s):  
Sarah MR Wille ◽  
Ann-Sofie ME Ingels ◽  
Nele Samyn
Keyword(s):  
Drug Testing ◽  
Oral Fluid ◽  
Dried Blood Spots ◽  
Blood Spots

scholarly journals Dietary Supplement and Food Contaminations and Their Implications for Doping Controls

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1012 ◽  
Author(s):  
Katja Walpurgis ◽  
Andreas Thomas ◽  
Hans Geyer ◽  
Ute Mareck ◽  
Mario Thevis
Keyword(s):  
Dietary Supplements ◽  
Drug Testing ◽  
Analytical Data ◽  
Good Manufacturing Practice ◽  
Therapeutic Drug ◽  
Positive Effects ◽  
Sports Drug Testing ◽  
And Performance ◽  
Androgenic Steroids ◽  
Result Management

A narrative review with an overall aim of indicating the current state of knowledge and the relevance concerning food and supplement contamination and/or adulteration with doping agents and the respective implications for sports drug testing is presented. The identification of a doping agent (or its metabolite) in sports drug testing samples constitutes a violation of the anti-doping rules defined by the World Anti-Doping Agency. Reasons for such Adverse Analytical Findings (AAFs) include the intentional misuse of performance-enhancing/banned drugs; however, also the scenario of inadvertent administrations of doping agents was proven in the past, caused by, amongst others, the ingestion of contaminated dietary supplements, drugs, or food. Even though controversial positions concerning the effectiveness of dietary supplements in healthy subjects exist, they are frequently used by athletes, anticipating positive effects on health, recovery, and performance. However, most supplement users are unaware of the fact that the administration of such products can be associated with unforeseeable health risks and AAFs in sports. In particular anabolic androgenic steroids (AAS) and stimulants have been frequently found as undeclared ingredients of dietary supplements, either as a result of cross-contaminations due to substandard manufacturing practices and missing quality controls or an intentional admixture to increase the effectiveness of the preparations. Cross-contaminations were also found to affect therapeutic drug preparations. While the sensitivity of assays employed to test pharmaceuticals for impurities is in accordance with good manufacturing practice guidelines allowing to exclude any physiological effects, minute trace amounts of contaminating compounds can still result in positive doping tests. In addition, food was found to be a potential source of unintentional doping, the most prominent example being meat tainted with the anabolic agent clenbuterol. The athletes’ compliance with anti-doping rules is frequently tested by routine doping controls. Different measures including offers of topical information and education of the athletes as well as the maintenance of databases summarizing low- or high-risk supplements are important cornerstones in preventing unintentional anti-doping rule violations. Further, the collection of additional analytical data has been shown to allow for supporting result management processes.

Therapeutic drug monitoring (TDM) of the recent antipsychotic ziprasidone in dried blood spots (DBS) and plasma

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
L Mercolini ◽  
G Fulgenzi ◽  
M Melis ◽  
G Boncompagni ◽  
LJ Albers ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Blood Spots

scholarly journals Multianalyte serology in home-sampled blood enables an unbiased assessment of the immune response against SARS-CoV-2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Niclas Roxhed ◽  
Annika Bendes ◽  
Matilda Dale ◽  
Cecilia Mattsson ◽  
Leo Hanke ◽  
...  
Keyword(s):  
Immune Response ◽  
Dried Blood Spots ◽  
Serological Testing ◽  
S Protein ◽  
Individual Level ◽  
Blood Spots

AbstractSerological testing is essential to curb the consequences of the COVID-19 pandemic. However, most assays are still limited to single analytes and samples collected within healthcare. Thus, we establish a multianalyte and multiplexed approach to reliably profile IgG and IgM levels against several versions of SARS-CoV-2 proteins (S, RBD, N) in home-sampled dried blood spots (DBS). We analyse DBS collected during spring of 2020 from 878 random and undiagnosed individuals from the population in Stockholm, Sweden, and use classification approaches to estimate an accumulated seroprevalence of 12.5% (95% CI: 10.3%–14.7%). This includes 5.4% of the samples being IgG+IgM+ against several SARS-CoV-2 proteins, as well as 2.1% being IgG−IgM+ and 5.0% being IgG+IgM− for the virus’ S protein. Subjects classified as IgG+ for several SARS-CoV-2 proteins report influenza-like symptoms more frequently than those being IgG+ for only the S protein (OR = 6.1; p < 0.001). Among all seropositive cases, 30% are asymptomatic. Our strategy enables an accurate individual-level and multiplexed assessment of antibodies in home-sampled blood, assisting our understanding about the undiagnosed seroprevalence and diversity of the immune response against the coronavirus.

Methylmercury Measurements in Dried Blood Spots from Electronic Waste Workers Sampled from Agbogbloshie, Ghana

2021 ◽  
Author(s):  
Andrea Santa Rios ◽  
Julius Fobil ◽  
Niladri Basu
Keyword(s):  
Electronic Waste ◽  
Dried Blood Spots ◽  
Blood Spots
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