scholarly journals Identification of naturally processed CD8 T cell epitopes from prostein, a prostate tissue-specific vaccine candidate

2004 ◽  
Vol 34 (4) ◽  
pp. 1091-1101 ◽  
Author(s):  
Rachel S. Friedman ◽  
A. Gregory Spies ◽  
Michael Kalos
Keyword(s):  
T Cell ◽  
Vaccine Candidate ◽  
Cd8 T Cell ◽  
Prostate Tissue ◽  
T Cell Epitopes ◽  
Tissue Specific

scholarly journals Link between Organ-specific Antigen Processing by 20S Proteasomes and CD8+ T Cell–mediated Autoimmunity

2002 ◽  
Vol 195 (8) ◽  
pp. 983-990 ◽  
Author(s):  
Ulrike Kuckelkorn ◽  
Thomas Ruppert ◽  
Britta Strehl ◽  
Peter R. Jungblut ◽  
Ursula Zimny-Arndt ◽  
...  
Keyword(s):  
T Cells ◽  
Small Intestine ◽  
T Cell ◽  
Cd8 T Cells ◽  
Antigen Processing ◽  
Specific Antigen ◽  
Cd8 T Cell ◽  
T Cell Epitopes ◽  
Tissue Specific ◽  
Organ Specific

Adoptive transfer of cross-reactive HSP60-specific CD8+ T cells into immunodeficient mice causes autoimmune intestinal pathology restricted to the small intestine. We wondered whether local immunopathology induced by CD8+ T cells can be explained by tissue-specific differences in proteasome-mediated processing of major histocompatibility complex class I T cell epitopes. Our experiments demonstrate that 20S proteasomes of different organs display a characteristic composition of α and β chain subunits and produce distinct peptide fragments with respect to both quality and quantity. Digests of HSP60 polypeptides by 20S proteasomes show most efficient generation of the pathology related CD8+ T cell epitope in the small intestine. Further, we demonstrate that the organ-specific potential to produce defined T cell epitopes reflects quantities that are relevant for cytotoxic T lymphocyte recognition. We propose tissue-specific antigen processing by 20S proteasomes as a potential mechanism to control organ-specific immune responses.

Metadherin peptides containing CD4+ and CD8+ T cell epitopes as a therapeutic vaccine candidate against cancer

2016 ◽  
Vol 60 (9) ◽  
pp. 646-652 ◽  
Author(s):  
Gourav Dhiman ◽  
Neha Lohia ◽  
Sahil Jain ◽  
Manoj Baranwal
Keyword(s):  
T Cell ◽  
Vaccine Candidate ◽  
Therapeutic Vaccine ◽  
Cd8 T Cell ◽  
T Cell Epitopes

HLA-B*27 subtype specificity determines the targeting of HLA-B*27 restricted HCV-specific CD8+ T cell epitopes

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
K Nitschke ◽  
J Schmidt ◽  
HE Blum ◽  
R Thimme ◽  
C Neumann-Haefelin
Keyword(s):  
T Cell ◽  
Cd8 T Cell ◽  
T Cell Epitopes ◽  
Subtype Specificity

scholarly journals Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 29
Author(s):  
Laia Bosch-Camós ◽  
Elisabet López ◽  
María Jesús Navas ◽  
Sonia Pina-Pedrero ◽  
Francesc Accensi ◽  
...  
Keyword(s):  
T Cell ◽  
Mononuclear Cells ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Cd8 T Cell ◽  
Protective Role ◽  
Full Length ◽  
T Cell Epitopes ◽  
Peripheral Blood Mononuclear

The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.

Identification of CD8+ T-cell Epitopes that Associate with Distinct Functionality, Proliferation and Polyfunctionality

2014 ◽  
Vol 30 (S1) ◽  
pp. A175-A175
Author(s):  
Meika EI Richmond ◽  
Sandra A. Kiazyk ◽  
Lyle R. Mckinnon ◽  
Billy Nyanga ◽  
Charles Wachihi ◽  
...  
Keyword(s):  
T Cell ◽  
Cd8 T Cell ◽  
T Cell Epitopes

High-throughput Screening for CD8+ T Cell Epitopes Targeted in Type 1 Diabetes

2010 ◽  
Vol 135 ◽  
pp. S19
Author(s):  
Brian Hondowicz ◽  
Katharine Schwedhelm ◽  
Arnold Kas ◽  
Michael Tasch ◽  
Nirasha Ramchurren ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
T Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cd8 T Cell ◽  
T Cell Epitopes

scholarly journals Extensive Polymorphism and Evidence of Immune Selection in a Highly Dominant Antigen Recognized by Bovine CD8 T Cells Specific for Theileria annulata

2011 ◽  
Vol 79 (5) ◽  
pp. 2059-2069 ◽  
Author(s):  
Niall D. MacHugh ◽  
William Weir ◽  
Alison Burrells ◽  
Regina Lizundia ◽  
Simon P. Graham ◽  
...  
Keyword(s):  
Amino Acid ◽  
T Cell ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Theileria Annulata ◽  
T Cell Epitopes ◽  
Major Histocompatibility Complex Haplotype ◽  
Content Type ◽  
Cell Responses ◽  
Restricted Epitope

ABSTRACTAlthough parasite strain-restricted CD8 T cell responses have been described for several protozoa, the precise role of antigenic variability in immunity is poorly understood. The tick-borne protozoan parasiteTheileria annulatainfects leukocytes and causes an acute, often fatal lymphoproliferative disease in cattle. Building on previous evidence of strain-restricted CD8 T cell responses toT. annulata, this study set out to identify and characterize the variability of the target antigens. Three antigens were identified by screening expressed parasite cDNAs with specific CD8 T cell lines. In cattle expressing the A10 class I major histocompatibility complex haplotype, A10-restricted CD8 T cell responses were shown to be focused entirely on a single dominant epitope in one of these antigens (Ta9). Sequencing of the Ta9 gene from field isolates ofT. annulatademonstrated extensive sequence divergence, resulting in amino acid polymorphism within the A10-restricted epitope and a second A14-restricted epitope. Statistical analysis of the allelic sequences revealed evidence of positive selection for amino acid substitutions within the region encoding the CD8 T cell epitopes. Sequence differences in the A10-restricted epitope were shown to result in differential recognition by individual CD8 T cell clones, while clones also differed in their ability to recognize different alleles. Moreover, the representation of these clonal specificities within the responding CD8 T cell populations differed between animals. As well as providing an explanation for incomplete protection observed after heterologous parasite challenge of vaccinated cattle, these results have important implications for the choice of antigens for the development of novel subunit vaccines.

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