Automatic medium exchange for micro‐volume cell samples based on dielectrophoresis

2021 ◽  
Author(s):  
Zhouyang Ma ◽  
Hongwang Zhao ◽  
Liujia Shi ◽  
Duli Yu ◽  
Xiaoliang Guo
Keyword(s):  
2012 ◽  
Vol 68 (1) ◽  
pp. 91-91 ◽  
Author(s):  
K. Röttger ◽  
A. Endriss ◽  
Jörg Ihringer ◽  
S. Doyle ◽  
W. F. Kuhs

In a previous paper we reported the lattice constants and thermal expansion of normal and deuterated ice Ih [Röttger et al. (1994). Acta Cryst. B50, 644–648]. Synchrotron X-ray powder diffraction data were used to obtain the lattice constants and unit-cell volumes of H2O and D2O ice Ih in the temperature range 15–265 K. A polynomial expression was given for the unit-cell volumes. It turns out that the coefficients quoted have an insufficient number of digits to faithfully reproduce the volume cell data. Here we provide a table with more significant digits. Moreover, we also provide the coefficients of a polynomial fit to the previously published a and c lattice constants of normal and deuterated ice Ih for the same temperature range.


2018 ◽  
Vol 47 (3) ◽  
pp. 958-970
Author(s):  
Roberto Fernández de Luis ◽  
Edurne S. Larrea ◽  
Joseba Orive ◽  
Arkaitz Fidalgo-Marijuan ◽  
Luis Lezama ◽  
...  

Cationic [Cu2(Tae)(Bpa)2]2+[NO3]22−·nH2O interpenetrated networks show a solvent loss triggering dynamic aperture of the porous structure from a closed to an open form with a difference of 29% of the volume cell.


Author(s):  
John Davenport

When exposed to water of low salinity specimens of Mytilus edulis L. keep their shell valves tightly closed; they do not gape periodically to test the external medium. Exchange of salts and water between the mantle cavity and the environment is thus minimized. Rising salinities are registered by diffusion of salts to the tentaculate portion of the inhalent siphon and not to any other portion of the mantle edge or to any more deeply located structures.


1995 ◽  
Vol 361 (4) ◽  
pp. 585-601 ◽  
Author(s):  
M. Dulce Madeira ◽  
N. Sousa ◽  
R. M. Santer ◽  
M. M. Paula-Barbosa ◽  
H. J. G. Gundersen

1978 ◽  
Vol 56 (2) ◽  
pp. 245-251 ◽  
Author(s):  
E. B. Vadas ◽  
E. A. Hosein

The effects of acute morphine administration on intact erythrocytes and on their flow properties were studied by measuring the mean cell volume, cell geometry, and whole blood and plasma viscosities. Morphine caused a small (2–7%) increase in mean cell volume. Changes in cell geometry were found to be time dependent and most pronounced in concave portions of the red cells. Whole blood viscosity was found to decrease upon morphine treatment; this may be due in part to a concurrent decrease in plasma viscosity.


1987 ◽  
Vol 42 (4) ◽  
pp. 408-413 ◽  
Author(s):  
H. Eikmeier ◽  
H. J. Rehm

Abstract The citric acid excretion of Ca-alginate-immobilized cells of Aspergillus niger in batch culture decreased with a half-time of approximately 19 days. Reactivation of the biocatalysts by regeneration in growth medium was possible, but it was followed by a submerged sporulation of the fungus, and medium was highly contaminated with free cells. Citric acid production could better be prolonged by semicontinuous cultivation with medium exchange every 7 or 14 days, respectively. After 32 days the remaining activity in semicontinuous culture was 1.4-fold higher than in comparable batch experiments. Similar improvements were obtained with a continuous process at a dilution rate of 0.125 v/v · d, whereby medium efflux completely free of detaching mycelia.


Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 744 ◽  
Author(s):  
Xiangchun Xuan

Magnetic field-induced particle manipulation is simple and economic as compared to other techniques (e.g., electric, acoustic, and optical) for lab-on-a-chip applications. However, traditional magnetic controls require the particles to be manipulated being magnetizable, which renders it necessary to magnetically label particles that are almost exclusively diamagnetic in nature. In the past decade, magnetic fluids including paramagnetic solutions and ferrofluids have been increasingly used in microfluidic devices to implement label-free manipulations of various types of particles (both synthetic and biological). We review herein the recent advances in this field with focus upon the continuous-flow particle manipulations. Specifically, we review the reported studies on the negative magnetophoresis-induced deflection, focusing, enrichment, separation, and medium exchange of diamagnetic particles in the continuous flow of magnetic fluids through microchannels.


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