Different grinding speeds affect induced regeneration capacity of human treated dentin matrix

Author(s):  
Min Li ◽  
Sen Yang ◽  
Jinlin Song ◽  
Tiwei Fu ◽  
Panpan Liang ◽  
...  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yaarit Kutsher ◽  
Michal Fisler ◽  
Adi Faigenboim ◽  
Moshe Reuveni

AbstractIt is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.


2018 ◽  
Vol 68 ◽  
pp. 34-40 ◽  
Author(s):  
Rafael Simões Gonçalves ◽  
Polliana Mendes Candia Scaffa ◽  
Marina Ciccone Giacomini ◽  
Marília Afonso Rabelo Buzalaf ◽  
Heitor Marques Honório ◽  
...  

1983 ◽  
Vol 62 (6) ◽  
pp. 713-714 ◽  
Author(s):  
Y. Kuboki ◽  
C.-F. Liu ◽  
T. Fusayama

The caries detector, 1.0% acid red sotution in proyylene glycol, failed to stain EDTA-demineralized bavine dentin matrix, but stained the same matrix when treated by lactic acid in a concentration above 0.01 M. Thus, acid of bacterial origin could possibly be responsible for the stainability change of carlous dentin in vivo.


2021 ◽  
Vol 13 (7) ◽  
pp. 4011
Author(s):  
Alfredo Sánchez-Bautista ◽  
Ester M. Palmero ◽  
Alberto J. Moya ◽  
Diego Gómez-Díaz ◽  
M. Dolores La Rubia

There are a lot of research programs focusing on the development of new solvents for carbon dioxide capture. The most important priority should be reducing the energy consumption needed at the regeneration step, but minimizing solvent degradation and its corrosivity is also considered as a priority. In this research, the aqueous blends of 2-amino-2-methyl-1-propanol (AMP: 1 kmol·m−3) and 1-amino-2-propanol (MIPA: 0.1–0.5 kmol·m−3) are characterized in terms of density, viscosity, and surface tension. The carbon dioxide absorption rate and capacity, the regeneration capacity, and the corrosivity of these solvents are also evaluated.


2001 ◽  
Vol 15 (1) ◽  
pp. 51-54 ◽  
Author(s):  
A.J. Smith ◽  
P.E. Murray ◽  
A.J. Sloan ◽  
J.B. Matthews ◽  
S. Zhao

Trans-dentinal stimulation of tertiary dentinogenesis has long been recognized, and has traditionally been ascribed to diffusion of irritant substances arising during injury and restorative treatment. Identification of bio-active components, especially growth factors including TGF-βs, sequestered within dentin matrix provides a new explanation for cellular signaling during tertiary dentinogenesis. Both isolated dentin matrix components and pure growth factors (TGF-βs) have been shown to signal cellular events leading to reactionary and reparative tertiary dentinogenesis. Release of these bio-active components from dentin matrix may arise during carious attack and other injury to the tissue, and also during subsequent surgical intervention and restoration of the tooth. Both cavity-conditioning agents and leaching from restorative materials may contribute to release of these components. Distance of diffusion, as determined by cavity residual dentin thickness, and other restorative parameters may influence the signaling process after release of these components. Careful consideration of the interplay between tissue injury and surgical and restorative material factors is required for optimum exploitation of the exquisite regenerative capacity of dentin-pulp for more biological approaches to clinical treatment of dental disease.


1997 ◽  
Vol 11 (4) ◽  
pp. 395-402
Author(s):  
J.J. Ten Bosch ◽  
J. De Vries ◽  
M.H. Van Der Veen

XPS, or ESCA, measures the atomic concentration in the outermost layers of the sample. The surface is often etched with Ar+-ions before any measurement. We investigated the role thereof on XPS measurements of root dentin. Nine slices were cut from premolars. Slices were ground or broken. XPS was measured before etching. Six samples were then subjected to Ar+-ion etching (10 kV ions, 0.25 μA/mm2). A gold sample was also included. Relative concentrations of N and C decreased with etching time, while 0, P, and Ca increased. N and C curves were analyzed assuming a sum of two exponential decays and a final level. On average, τ1 = 20 sec for C and 17 sec for N. On average, τ2 = 278 sec for C and 350 sec for N. No differences between differently prepared samples were apparent. The gold sample showed a single decay to noise for C and N, with τ1 = 8 and 7 sec, respectively. We conclude that two decay processes are present, due to gas removal and to the removal of organic material from the dentin matrix, respectively. Thus, true values can be obtained by extrapolation to t = 0 of only the data obtained by summing the slow decay and the final level.


Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2166
Author(s):  
Jeong-Kui Ku ◽  
In-Woong Um ◽  
Mi-Kyoung Jun ◽  
Il-hyung Kim

An autogenous, demineralized, dentin matrix is a well-known osteo-inductive bone substitute that is mostly composed of type I collagen and is widely used in implant dentistry. This single case report describes a successful outcome in guided bone regeneration and dental implantation with a novel human-derived collagen membrane. The authors fabricated a dentin-derived-barrier membrane from a block-type autogenous demineralized dentin matrix to overcome the mechanical instability of the collagen membrane. The dentin-derived-barrier acted as an osteo-inductive collagen membrane with mechanical and clot stabilities, and it replaced the osteo-genetic function of the periosteum. Further research involving large numbers of patients should be conducted to evaluate bone forming capacity in comparison with other collagen membranes.


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