Activation of EGFR promotes squamous carcinoma SCC10A cell migration and invasion via inducing EMT-like phenotype change and MMP-9-mediated degradation of E-cadherin

2011 ◽  
Vol 112 (9) ◽  
pp. 2508-2517 ◽  
Author(s):  
Jian-Hong Zuo ◽  
Wei Zhu ◽  
Mao-Yu Li ◽  
Xin-Hui Li ◽  
Hong Yi ◽  
...  
Keyword(s):  
Cell Migration ◽  
Squamous Carcinoma ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
E Cadherin

scholarly journals Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

BMC Urology ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Cai Lv ◽  
Yuan Huang ◽  
Qingqing Lei ◽  
Zhenxiang Liu ◽  
Shixing Shen ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Migration ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Normal Tissue ◽  
Negative Control ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Interfering Rna ◽  
E Cadherin

Abstract Background The metastasis-associated gene 1 (MTA1) has been extensively reported as a crucial oncogene, and its abnormal expression has been associated with the progression of numerous cancers. However, the role of MTA1 in renal cell carcinoma (RCC) progression and metastasis remains unclear. Herein, we investigated the expression of MTA1 and its role in RCC. Methods 109 matched clear cell RCCs (ccRCCs) and corresponding normal tissue samples were analyzed via immunohistochemistry to test the expression of MTA1. Human A498 cell lines were transfected with pcDNA3.1-Flag (control) or Flag-MTA1 to overexpress MTA1 or with specific interfering RNA (si-MTA1) or specific interfering negative control to knockdown MTA1 expression. Transfected cells were used in wound healing and transwell invasion assay. Quantitative real time polymerase chain reaction was used to assess the effect of MTA1 on MMP2/MMP9 and E-cadherin gene expression. Western blot was used to qualify the phosphorylation of p65. Results Herein, we found a significantly increased expression of MTA1 in 109 ccRCCs, compared to the corresponding normal tissue. In addition, the overexpression of MTA1 in A498 cells facilitated cell migration and invasion, while the down-regulation of MTA1 expression using specific interfering RNA sequences could decrease cell migration and invasion. Furthermore, we showed that MTA1 is up-regulated in ccRCCs, which contributes to the migration and invasion of human kidney cancer cells by mediating the expression of MMP2 and MMP9 through the NF-κB signaling pathway. Similarly, we found that MTA1 could regulate E-cadherin expression in RCCs. Conclusions MTA1 is overexpressed in RCC and is involved in the progression of RCC through NF-κB.

scholarly journals MicroRNA-1275 inhibits cell migration and invasion in gastric cancer by regulating vimentin and E-cadherin via JAZF1

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jia-Wei Mei ◽  
Zi-Yi Yang ◽  
Hong-Gang Xiang ◽  
Runfa Bao ◽  
Yuan-Yuan Ye ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Migration ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
E Cadherin

Abstract 2332: The new protein FAM110B regulates the expression of E-cadherin and is involved in cell migration and invasion

2011 ◽  
Author(s):  
Mehdi Naouar ◽  
Danièle Montaudon ◽  
Vincent Verbiest ◽  
Audrey Kauffmann ◽  
Audrey Laroche-Clary ◽  
...  
Keyword(s):  
Cell Migration ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
New Protein ◽  
E Cadherin

scholarly journals p110 CUX1 Homeodomain Protein Stimulates Cell Migration and Invasion in Part through a Regulatory Cascade Culminating in the Repression of E-cadherin and Occludin

2009 ◽  
Vol 284 (40) ◽  
pp. 27701-27711 ◽  
Author(s):  
Valerie Kedinger ◽  
Laurent Sansregret ◽  
Ryoko Harada ◽  
Charles Vadnais ◽  
Chantal Cadieux ◽  
...  
Keyword(s):  
Cell Migration ◽  
Migration And Invasion ◽  
Homeodomain Protein ◽  
Cell Migration And Invasion ◽  
Regulatory Cascade ◽  
E Cadherin

488 SNAIL TRANSFECTION INDUCES EPITHELIAL-MESENCHYMAL TRANSITION WITH PROPERTIES OF CANCER STEM-LIKE CELL IN ESOPHAGEAL SQUAMOUS CELL CANCER

2021 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Ching Tzao ◽  
Meng-Hsun Wu ◽  
Ben-Hen Chen
Keyword(s):  
Cell Migration ◽  
Cell Lines ◽  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Cell Cancer ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Cell Migration And Invasion ◽  
Sphere Formation ◽  
E Cadherin

Abstract   Cancer stem-like cell (CSC) is an important player in tumorigenesis and tumor progression. Snail has been demonstrated as a key driver for epithelial-mesenchymal transition (EMT) that is closely linked with generation of stem-like cell in human cancer. We aim to investigate if Snail transfection induces EMT and properties of stem-like cell in esophageal squamous cell carcinoma (ESCC) cell lines. Methods A lentivirus system was used to transfect human Snail via a plasmid pLV-EF1a-hSnail-Hyg into KYSE-170 and KYSE-510 ESCC cell lines. Immunoblotting was used to determine expression of EMT associated markers including Vimentin, E-cadherin, Fibronectin and N-cadherin. Assays for cell migration and invasion were conducted in Snail-transfected cells and its vector control. Cytotoxicity (MTT) and cell proliferation assay was used to determine cell growth. Sphere formation assay and flow cytometry (FCM) were employed to characterize stem cell properties while examining expression of stemness genes by using real-time polymerase chain reaction (PCR). Results After Snail transfection, mesenchymal markers, Vimentin, N-cadherin increased, whereas epithelial marker E-cadherin decreased. Snail-transfected. ESCC cells presented a significant increase in RNA expression of stemness genes such as Nanog, Oct4, ABCG2 and Sox2 with an induction in sphere formation. Moreover, ability of cell migration and invasion increased after Snail-transfection in ESCC with increased chemoresistance to cisplatin, taxol, and 5-Fluorouracil(5-FU) and an increase in radioresistance as well. Conclusion Our results demonstrated that Snail transfection induced EMT with properties of CSC in ESCC cell lines.

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