Lipid conversion of Scenedesmus rubescens biomass into biodiesel using biochar catalysts from malt spent rootlets

2020 ◽  
Vol 95 (9) ◽  
pp. 2421-2429 ◽  
Author(s):  
Vasiliki D Tsavatopoulou ◽  
John Vakros ◽  
Ioannis D Manariotis

2014 ◽  
Vol 38 (2) ◽  
pp. 379-387 ◽  
Author(s):  
Isabella Moro ◽  
Valerio Matozzo ◽  
Anna Piovan ◽  
Emanuela Moschin ◽  
Francesca Dalla Vecchia






2018 ◽  
Vol 40 (2) ◽  
pp. 187-192 ◽  
Author(s):  
R. Campiche ◽  
P. Sandau ◽  
E. Kurth ◽  
M. Massironi ◽  
D. Imfeld ◽  
...  


Photonics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 42
Author(s):  
Konstantina Tourlouki ◽  
Vasiliki Tsavatopoulou ◽  
Dimitris Alexandropoulos ◽  
Ioannis D. Manariotis ◽  
Simone Mazzucato

Microalgae are an ideal source for next-generation biofuels due to their high photosynthetic rate. However, a key process limitation in microalgal biofuel production is harvesting of biomass and extraction of lipids in a cost-effective manner. The harvesting of the algal biomass amounts to approximately 20 to 30% of the total cost of the cultivation; hence, developing an efficient and universal harvesting method will make the commercialization of microalgal bio-cultures sustainable. In this study, we developed, demonstrated, and evaluated a novel harvesting method based on Glass Reinforced Fiber Polymer (GFRP) panels, suitable for industrial-scale installations. The proposed method was based on previous observations of preferential micro-algae development on glass surfaces, as well as in the assumption that the microalgae cells would prefer to attach to and grow on substrates with a similar size as them. At first, we developed a laser micromachining protocol for removing the resin and revealing the glass fibers of the GFRP, available for algal adhesion, thus acting as a microalgae biomass harvesting center. Surface micromachining was realized using a ns pulsed ultraviolet laser emitting at 355 nm. This laser ensured high machining quality of the GFRP, because of its selective material ablation, precise energy deposition, and narrow heat affected zone. A specially built open pond system was used for the cultivation of the microalgae species Scenedesmus rubescens, which was suitable for biofuel production. The cultivation was used for the experimental evaluation of the proposed harvesting method. The cultivation duration was set to 16 days in order for the culture to operate at the exponential growth phase. The biomass maximum recovery due to microalgae attachment on the GFRP surface was 13.54 g/m2, a yield comparable to other studies in the literature. Furthermore, the GFRP surfaces could be upscaled to industrial dimensions and positioned in any geometry dictated by the photobioreactor design. In this study, the glass fiber reinforced polymer used was suitable for the adhesion of Scenedesmus rubescens due to its fiber thickness. Other microalgae species could be cultivated, adhere, and harvested using GFRP of different fiber sizes and/or with a modified laser treatment. These very encouraging results validated GFRPs’ harvesting capabilities as an attachment substrate for microalgae. Additional studies with more algae species will further strengthen the method.



Energies ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4895
Author(s):  
Joseph Christian Utomo ◽  
Young Mo Kim ◽  
Hyun Uk Cho ◽  
Jong Moon Park

This study examined the feasibility of using non-sterilized swine wastewater for lipid production by an isolated microalga, Scenedesmus rubescens. Different dilution ratios using municipal wastewater as a diluent were tested to determine the suitable levels of microalgal growth in the wastewaters, its nutrient removal, and its lipid production. The highest lipid productivity (8.37 mg/L/d) and NH4+ removal (76.49%) were achieved in swine wastewater that had been diluted to 30 times using municipal wastewater. Various bacteria coexisted in the wastewaters during the cultivation of S. rubescens. These results suggest the practical feasibility of a system to produce lipids from swine wastewater by using microalgae.





Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2262
Author(s):  
Andriana F. Aravantinou ◽  
Fytoula Andreou ◽  
Ioannis D. Manariotis

The scope of this study was to investigate the toxic effects of zinc oxide (ZnO) nanoparticles (NPs) on freshwater microalgae, in long-term semi-batch feeding mode at two different hydraulic retention times (HRTs) (20 and 40 days). A freshwater microalgae, Scenedesmus rubescens, was employed and exposed to a semi-continuous supply of ZnO NPs at a low concentration of 0.081 mg/L for a period of 28 d. Experiments were conducted under controlled environmental conditions. Τhe impact of ZnO NPs on S. rubescens, which was assessed in terms of nutrient removal, biomass growth, and algal lipid content. Semi-batch mode cultures showed that low ZnO NP concentrations at an HRT of 40 d did not have any negative effect on microalgae growth after the fourth day of culture. In contrast, algal growth was inhibited up to 17.5% at an HRT of 20 d in the presence of ZnO NPs. This might be attributed to the higher flow rate applied and ZnO NPs load. A positive correlation between nutrient removal and microalgae growth was observed. The algal lipid content was, in most cases, higher in the presence of ZnO NPs at both HRTs, indicating that even low ZnO NPs concentration cause stress resulting in higher lipid content.



Energies ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1336 ◽  
Author(s):  
Michael Kröger ◽  
Marco Klemm ◽  
Michael Nelles

Microalgae utilized for experiments are often not produced by the researchers that are doing experiments with them. The microalgae are made storable through thermal or freeze-drying by the producer. In an industrial scaled process, because of efficiency reasons, microalgae would not be dried, but processed directly. With that, the question is, if drying already could change the composition or structure that much, that a process scaled up from laboratory to productive scale with fresh microalgae would be less efficient or even would not work at all. The effect of freeze drying on the extraction behavior for the species Scenedesmus rubescens was investigated. It was obtained in freeze-dried condition and again was delivered in fresh state. The utilized microalgae were extracted with n-hexane, without and with different pretreatments (acidic hydrolysis and hydrothermal carbonization) to examine the differences in the yields. In conclusion, it was demonstrated that freeze drying harms the cell wall and therefore this process already influences the quantity of extracted lipids. Depending on the harshness of the treatment process for cell wall disruption this might influence the extracted yield when the algae are not freeze-dried. The quality of the extracted lipids does not change when freeze-dried.



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