Bioremediation of petroleum hydrocarbons by alkali–salt‐tolerant microbial consortia and their community profiles

2020 ◽  
Author(s):  
Xuwang Zhang ◽  
Dongli Bao ◽  
Maoting Li ◽  
Qidong Tang ◽  
Minghuo Wu ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Microbial Consortia ◽  
Salt Tolerant ◽  
Alkali Salt

Biodegradation of different petroleum hydrocarbons by free and immobilized microbial consortia

2015 ◽  
Vol 17 (12) ◽  
pp. 2022-2033 ◽  
Author(s):  
Tiantian Shen ◽  
Yongrui Pi ◽  
Mutai Bao ◽  
Nana Xu ◽  
Yiming Li ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Microbial Consortia ◽  
Biodegradation Rate

The biodegradation rate of crude oil by semi-coke immobilized microbial consortia was higher than that by free microbial consortia.

Microbial Consortia and Biodegradation of Petroleum Hydrocarbons in Marine Environments

2018 ◽  
pp. 1-20 ◽  
Author(s):  
J. Paniagua-Michel ◽  
Babu Z. Fathepure
Keyword(s):  
Petroleum Hydrocarbons ◽  
Microbial Consortia ◽  
Marine Environments

Diagnostic and resulting approaches to restore petroleum-contaminated soil in a Mexican tropical swamp

2000 ◽  
Vol 42 (5-6) ◽  
pp. 377-384 ◽  
Author(s):  
M. Gallegos Martínez ◽  
A. Gómez Santos ◽  
L. González Cruz ◽  
M.A. Montes de Oca García ◽  
L. Yáñez Trujillo ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Total Petroleum Hydrocarbons ◽  
Microbial Consortia ◽  
Contaminated Site ◽  
Second Phase ◽  
Remote Sensors ◽  
Petroleum Contaminated Soil ◽  
Low Sensitivity ◽  
Oil Company

A multidisciplinary three-step methodology is being developed to diagnose the extent and type of petroleum pollutants and resulting technological approaches to restore a contaminated site. At first, the site was delimitated and its zones identified by using remote sensors. An area of 307 ha considered of major importance to the national Mexican oil company, Petróleos Mexicanos (PEMEX), was identified. 75% of total analyzed soil samples ranged between 10-50,000 ppm of total petroleum hydrocarbons (TPH) and 25% between 50,000 and 434,000 ppm. Aliphatic and asphaltene groups were predominant and technological alternatives were proposed. In a second phase the identification of native botanical and microbial capabilities to biodegrade pollutants was achieved. Three native botanical species were selected for greenhouse studies: Cyperus laxus showed low sensitivity to TPH resulting in higher seed germination efficiency and growth rate. Since microbial consortia isolated from C. laxus rhizosphere were able todegrade up to 70% of TPH in 30 days laboratory cultures, a phytoremediation-reforest alternative was finally proposed to PEMEX. In a third step, the construction of a pilot plant in situ is now in course wherein both treatability studies and reforest strategies are being developed.

scholarly journals Petroleum Hydrocarbon Contamination in Terrestrial Ecosystems—Fate and Microbial Responses

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3400 ◽  
Author(s):  
Adam Truskewycz ◽  
Taylor D. Gundry ◽  
Leadin S. Khudur ◽  
Adam Kolobaric ◽  
Mohamed Taha ◽  
...  
Keyword(s):  
Natural Attenuation ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Terrestrial Ecosystems ◽  
Hydrocarbon Contamination ◽  
Microbial Consortia ◽  
Metagenomic Sequencing ◽  
Petroleum Hydrocarbon Contamination ◽  
The Impact

Petroleum hydrocarbons represent the most frequent environmental contaminant. The introduction of petroleum hydrocarbons into a pristine environment immediately changes the nature of that environment, resulting in reduced ecosystem functionality. Natural attenuation represents the single, most important biological process which removes petroleum hydrocarbons from the environment. It is a process where microorganisms present at the site degrade the organic contaminants without the input of external bioremediation enhancers (i.e., electron donors, electron acceptors, other microorganisms or nutrients). So successful is this natural attenuation process that in environmental biotechnology, bioremediation has developed steadily over the past 50 years based on this natural biodegradation process. Bioremediation is recognized as the most environmentally friendly remediation approach for the removal of petroleum hydrocarbons from an environment as it does not require intensive chemical, mechanical, and costly interventions. However, it is under-utilized as a commercial remediation strategy due to incomplete hydrocarbon catabolism and lengthy remediation times when compared with rival technologies. This review aims to describe the fate of petroleum hydrocarbons in the environment and discuss their interactions with abiotic and biotic components of the environment under both aerobic and anaerobic conditions. Furthermore, the mechanisms for dealing with petroleum hydrocarbon contamination in the environment will be examined. When petroleum hydrocarbons contaminate land, they start to interact with its surrounding, including physical (dispersion), physiochemical (evaporation, dissolution, sorption), chemical (photo-oxidation, auto-oxidation), and biological (plant and microbial catabolism of hydrocarbons) interactions. As microorganism (including bacteria and fungi) play an important role in the degradation of petroleum hydrocarbons, investigations into the microbial communities within contaminated soils is essential for any bioremediation project. This review highlights the fate of petroleum hydrocarbons in tertial environments, as well as the contributions of different microbial consortia for optimum petroleum hydrocarbon bioremediation potential. The impact of high-throughput metagenomic sequencing in determining the underlying degradation mechanisms is also discussed. This knowledge will aid the development of more efficient, cost-effective commercial bioremediation technologies.

scholarly journals Quantifying Microbial Utilization of Petroleum Hydrocarbons in Salt Marsh Sediments by Using the 13C Content of Bacterial rRNA

2007 ◽  
Vol 74 (4) ◽  
pp. 1157-1166 ◽  
Author(s):  
Ann Pearson ◽  
Kimberly S. Kraunz ◽  
Alex L. Sessions ◽  
Anne E. Dekas ◽  
William D. Leavitt ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Petroleum Hydrocarbons ◽  
Oil Spills ◽  
Bacterial Biomass ◽  
Magnetic Bead ◽  
16S Rrna Genes ◽  
Microbial Consortia ◽  
Rrna Genes ◽  
Carbon Isotopic ◽  
Microbial Utilization

ABSTRACT Natural remediation of oil spills is catalyzed by complex microbial consortia. Here we took a whole-community approach to investigate bacterial incorporation of petroleum hydrocarbons from a simulated oil spill. We utilized the natural difference in carbon isotopic abundance between a salt marsh ecosystem supported by the 13C-enriched C4 grass Spartina alterniflora and 13C-depleted petroleum to monitor changes in the 13C content of biomass. Magnetic bead capture methods for selective recovery of bacterial RNA were used to monitor the 13C content of bacterial biomass during a 2-week experiment. The data show that by the end of the experiment, up to 26% of bacterial biomass was derived from consumption of the freshly spilled oil. The results contrast with the inertness of a nearby relict spill, which occurred in 1969 in West Falmouth, MA. Sequences of 16S rRNA genes from our experimental samples also were consistent with previous reports suggesting the importance of Gamma- and Deltaproteobacteria and Firmicutes in the remineralization of hydrocarbons. The magnetic bead capture approach makes it possible to quantify uptake of petroleum hydrocarbons by microbes in situ. Although employed here at the domain level, RNA capture procedures can be highly specific. The same strategy could be used with genus-level specificity, something which is not currently possible using the 13C content of biomarker lipids.

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

Managing the risks from petroleum hydrocarbons at contaminated sites: achievements and future research directions

2005 ◽  
Vol 13 (2) ◽  
pp. 115-122 ◽  
Author(s):  
S.J.T. Pollard ◽  
R. Duarte-Davidson ◽  
K. Askari ◽  
E. Stutt
Keyword(s):  
Petroleum Hydrocarbons ◽  
Contaminated Sites ◽  
Future Research ◽  
Research Directions ◽  
Future Research Directions

Development of Salt Tolerant Eucalyptus Globulus by Selection

2006 ◽  
Vol 60 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Akira Murakami
Keyword(s):  
Eucalyptus Globulus ◽  
Salt Tolerant
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