Q-FIHC: Quantification of fluorescence immunohistochemistry to analysep63 isoforms and cell cycle phases in human limbal stem cells

2006 ◽  
Vol 69 (12) ◽  
pp. 983-991 ◽  
Author(s):  
Enzo Di Iorio ◽  
Vanessa Barbaro ◽  
Stefano Ferrari ◽  
Claudio Ortolani ◽  
Michele De Luca ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Limbal Stem Cells ◽  
Cell Cycle Phases ◽  
Fluorescence Immunohistochemistry

scholarly journals Autophagy mediates cell cycle response by regulating nucleocytoplasmic transport of PAX6 in limbal stem cells under ultraviolet-A stress

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180868 ◽  
Author(s):  
Maria Laggner ◽  
Andreas Pollreisz ◽  
Gerald Schmidinger ◽  
Ursula Schmidt-Erfurth ◽  
Ying-Ting Chen
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Nucleocytoplasmic Transport ◽  
Ultraviolet A ◽  
Limbal Stem Cells

scholarly journals Fluorescent indicators for continuous and lineage‐specific reporting of cell‐cycle phases in human pluripotent stem cells

2020 ◽  
Vol 117 (7) ◽  
pp. 2177-2186
Author(s):  
Yun Chang ◽  
Peter B. Hellwarth ◽  
Lauren N. Randolph ◽  
Yufei Sun ◽  
Yuxian Xing ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Fluorescent Indicators ◽  
Cell Cycle Phases

scholarly journals C/EBPδ regulates cell cycle and self-renewal of human limbal stem cells

2007 ◽  
Vol 177 (6) ◽  
pp. 1037-1049 ◽  
Author(s):  
Vanessa Barbaro ◽  
Anna Testa ◽  
Enzo Di Iorio ◽  
Fulvio Mavilio ◽  
Graziella Pellegrini ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Clonal Evolution ◽  
Limbal Stem Cells ◽  
Direct Role ◽  
Self Renewal ◽  
Corneal Injury ◽  
Enhancer Binding Protein ◽  
Limbal Stem Cell

Human limbal stem cells produce transit amplifying progenitors that migrate centripetally to regenerate the corneal epithelium. Coexpression of CCAAT enhancer binding protein δ (C/EBPδ), Bmi1, and ΔNp63α identifies mitotically quiescent limbal stem cells, which generate holoclones in culture. Upon corneal injury, a fraction of these cells switches off C/EBPδ and Bmi1, proliferates, and differentiates into mature corneal cells. Forced expression of C/EBPδ inhibits the growth of limbal colonies and increases the cell cycle length of primary limbal cells through the activity of p27Kip1 and p57Kip2. These effects are reversible; do not alter the limbal cell proliferative capacity; and are not due to apoptosis, senescence, or differentiation. C/EBPδ, but not ΔNp63α, indefinitely promotes holoclone self-renewal and prevents clonal evolution, suggesting that self-renewal and proliferation are distinct, albeit related, processes in limbal stem cells. C/EBPδ is recruited to the chromatin of positively (p27Kip1 and p57Kip2) and negatively (p16INK4A and involucrin) regulated gene loci, suggesting a direct role of this transcription factor in determining limbal stem cell identity.

Effects of cell cycle phases on the induction of dental pulp stem cells toward dopaminergic‐like cells

2017 ◽  
Vol 12 (2) ◽  
Author(s):  
Nareshwaran Gnanasegaran ◽  
Vijayendran Govindasamy ◽  
Premasangery Kathirvaloo ◽  
Sabri Musa ◽  
Noor Hayaty Abu Kasim
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Cell Cycle Phases

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

High Glucose Affects the Cytotoxic Potential of Rapamycin, Metformin and Hydrogen Peroxide in Cultured Human Mesenchymal Stem Cells

2019 ◽  
Vol 19 (9) ◽  
pp. 688-698 ◽  
Author(s):  
Azam Roohi ◽  
Mahin Nikougoftar ◽  
Hamed Montazeri ◽  
Shadisadat Navabi ◽  
Fazel Shokri ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
High Glucose ◽  
Cell Cycle Distribution ◽  
Efficient Treatment ◽  
Chronic Hyperglycemia ◽  
Placental Mesenchymal Stem Cells

Background: Oxidative stress and chronic hyperglycemia are two major side effects of type 2 diabetes affecting all cell types including mesenchymal stem cells (MSCs). As a cell therapy choice, understanding the behavior of MSCs will provide crucial information for efficient treatment. Methods: Placental mesenchymal stem cells were treated with various concentrations of glucose, metformin, rapamycin, and hydrogen peroxide to monitor their viability and cell cycle distribution. Cellular viability was examined via the MTT assay. Cell cycle distribution was studied by propidium iodide staining and apoptosis was determined using Annexin Vpropidium iodide staining and flow cytometry. Involvement of potential signaling pathways was evaluated by Western blotting for activation of Akt, P70S6K, and AMPK. Results: The results indicated that high glucose augmented cell viability and reduced metformin toxic potential. However, the hydrogen peroxide and rapamycin toxicities were exacerbated. Conclusion: Our findings suggest that high glucose concentration has a major effect on placental mesenchymal stem cell viability in the presence of rapamycin, metformin and hydrogen peroxide in culture.

Hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in HepG2 cells

2020 ◽  
Vol 01 ◽  
Author(s):  
Ayşe Mine Yılmaz ◽  
Gökhan Biçim ◽  
Kübra Toprak ◽  
Betül Karademir Yılmaz ◽  
Irina Milisav ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Hepg2 Cells ◽  
Proteasome Activity ◽  
Cell Cycle Phases ◽  
Induced Changes ◽  
And Oxidative Stress ◽  
Quercetin Treatment

Background: Different cellular responses influence the progress of cancer. In this study, we have investigated the effect of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in human hepatocellular carcinoma (HepG2) cells. Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50 and proteasome activity were determined by Western blot and fluorometry, respectively. Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment. Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.

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