HBV genotype C strains with spontaneous YMDD mutations may be a risk factor for hepatocellular carcinoma

Abstract Objective To investigate the occurrence of basal core promoter (BCP) and pre-C mutations in patients with hepatitis B virus (HBV) infection in Gansu Province, China, and to analyze the correlation of HBV mutation and HBV genotype with primary hepatocellular carcinoma (HCC). Methods PCR-RFLP was applied to detect HBV subgenotypes, and the presence of the pre-C and BCP mutations in 62 patients with HCC, 70 patients with hepatitis B induced liver cirrhosis (LC) and 90 patients with chronic hepatitis B (CHB). Results In HCC patients, genotype C was the major genotype (70.97%). The pre-C mutation was found in 59.68%, 31.43% and 16.67% patients with HCC, LC and CHB, respectively. The frequency of BCP mutations was significantly different between patients with HCC, LC and CHB (74.19%, 51.43% and 37.78%, respectively; X2=30.727, 19.540, respectively, P < 0.01). Patients in HCC group had a higher incidence of pre-C as well as BCP mutations compared to the other groups. The prevalence of pre-C and BCP mutations was significantly higher in patients with genotype C1 (44.32% and 69.32%, respectively) compared to patients with other subgenotypes (P < 0.05). Conclusions The incidence of pre-C and BCP mutations increases with disease progression. Pre-C and BCP mutations frequently occur in patients with genotype C1. HBV genotype C, pre-C mutations and BCP mutations are closely related to the occurrence of HCC.

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High Viral Load and Hepatitis B Virus Subgenotype Ce Are Associated With Increased Risk of Hepatocellular Carcinoma

Journal of Clinical Oncology ◽

10.1200/jco.2007.13.2043 ◽

2008 ◽

Vol 26 (2) ◽

pp. 177-182 ◽

Cited By ~ 200

Author(s):

Henry Lik-Yuen Chan ◽

Chi-Hang Tse ◽

Frankie Mo ◽

Jane Koh ◽

Vincent Wai-Sun Wong ◽

...

Keyword(s):

Risk Factors ◽

Hepatocellular Carcinoma ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Hbv Dna ◽

Hazard Ratio ◽

Hbv Genotype ◽

Hbv Genotypes ◽

B Virus ◽

Genotype C

Purpose We aimed to investigate the impact of hepatitis B virus (HBV) DNA and HBV genotypes/subgenotypes on the risk of hepatocellular carcinoma (HCC). Patients and Methods A prospective cohort of patients infected with chronic HBV in a surveillance program for HCC since 1997 was studied. Ultrasound and alpha-fetoprotein evaluation were regularly performed to detect HCC. Risk factors for HCC and the relationship between HBV DNA and HBV genotypes were determined. Results Among 1,006 patients with a median follow-up of 7.7 years, 86 patients (8.5%) developed HCC. With reference to the low HBV DNA stratum (log HBV DNA ≤ 4.5 copies/mL), the hazard ratio for HCC of the intermediate HBV DNA stratum (log HBV DNA > 4.5 to 6.5 copies/mL) was 1.62 (95% CI, 1.05 to 2.48; P = .027) and that of the high HBV DNA stratum (log HBV DNA > 6.5 copies/mL) was 2.73 (95% CI, 1.76 to 4.25; P < .001). Among patients with genotyping results, 330 patients had HBV genotype B and 439 patients had HBV genotype C (94 subgenotype Ce and 345 subgenotype Cs). With reference to HBV genotype B, HBV subgenotype Ce has the highest risk of HCC (hazard ratio = 2.75; 95% CI, 1.66 to 4.56; P < .0001) and HBV subgenotype Cs has intermediate risk (hazard ratio = 1.70; 95% CI, 1.09 to 2.64; P = .020). On multivariate analysis, HBV DNA, HBV genotypes, liver cirrhosis, male sex, older age, and lower serum albumin were independent risk factors of HCC. Conclusion High HBV DNA level and HBV genotype C, particularly subgenotype Ce, increased the risk of HCC in chronic hepatitis B.

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Hepatic iron accumulation is an independent risk factor for the development of hepatocellular carcinoma after interferon therapy in patients with chronic hepatitis C

Gastroenterology ◽

10.1016/s0016-5085(01)80381-4 ◽

2001 ◽

Vol 120 (5) ◽

pp. A77-A77

Author(s):

N IZUMI ◽

Y ASAHINA ◽

M UCHIHARA ◽

N OSAMU ◽

N KANAZAWA ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Chronic Hepatitis ◽

Risk Factor ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Independent Risk Factor ◽

Interferon Therapy ◽

Iron Accumulation ◽

Hepatic Iron

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Mechanisms of liver damage in COVID-19

Medical alphabet ◽

10.33667/2078-5631-2020-19-39-46 ◽

2020 ◽

Vol 1 (19) ◽

pp. 39-46

Author(s):

T. V. Pinchuk ◽

N. V. Orlova ◽

T. G. Suranova ◽

T. I. Bonkalo

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Transplantation ◽

Risk Factor ◽

Liver Disease ◽

Liver Function ◽

Liver Damage ◽

The World ◽

Coronavirus Infection ◽

The Impact ◽

Analyze Data

At the end of 2019, a new coronavirus (SARS-CoV-2) was discovered in China, causing the coronavirus infection COVID-19. The ongoing COVID-19 pandemic poses a major challenge to health systems around the world. There is still little information on how infection affects liver function and the significance of pre-existing liver disease as a risk factor for infection and severe COVID-19. In addition, some drugs used to treat the new coronavirus infection are hepatotoxic. In this article, we analyze data on the impact of COVID-19 on liver function, as well as on the course and outcome of COVID-19 in patients with liver disease, including hepatocellular carcinoma, or those on immunosuppressive therapy after liver transplantation.

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Faculty Opinions recommendation of Obesity is an independent risk factor for hepatocellular carcinoma development in chronic hepatitis C patients.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1112156.568142 ◽

2008 ◽

Author(s):

Dieter Juengst

Keyword(s):

Hepatocellular Carcinoma ◽

Chronic Hepatitis ◽

Risk Factor ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Independent Risk Factor ◽

Chronic Hepatitis C Patients

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Study of Toll-like Receptor 3 Gene Polymorphism as a Novel Risk Factor for HCV-related Hepatocellular Carcinoma in Egypt

Current Cancer Drug Targets ◽

10.2174/1568009620666200319102929 ◽

2020 ◽

Vol 20 (5) ◽

pp. 382-389 ◽

Cited By ~ 1

Author(s):

Shimaa EL-Sharawy ◽

Osama El- Sayed Negm ◽

Sherief Abd-Elsalam ◽

Hesham Ahmed EL-Sorogy ◽

Mona Ahmed Helmy Shehata

Keyword(s):

Hepatocellular Carcinoma ◽

Risk Factor ◽

Gene Polymorphism ◽

Clinical Laboratory ◽

Treatment Options ◽

Laboratory Evaluation ◽

Toll Like Receptor ◽

Focal Lesions ◽

Tlr3 Gene ◽

Cirrhotic Patients

Background & Aims: Hepatocellular carcinoma (HCC) is a highly aggressive cancer with few treatment options. Toll-like receptor 3 (TLR3) plays a key role in innate immunity and may affect the development of cancers. This study aimed to investigate the association between TLR3 gene polymorphism and HCV-related hepatocellular carcinoma in Egypt. Methods: This work was conducted on 70 individuals; fifty HCV cirrhotic patients were included in two groups; with HCC (30 patients) and without HCC (20 patients) compared with a group of 20 apparently healthy controls. All of the studied individuals underwent clinical-laboratory evaluation. TLR3 gene single-nucleotide polymorphism (SNP) (+1234C/T) was tested by polymerase chain reaction- restriction fragment length polymorphism. Results: This study reported that the prevalence of TLR3 +1234TT genotype was significantly increased in cirrhotic patients with HCC than without HCC, while it was not detected at all among the controls. When analyzing the TLR3 SNP +1234C/T with different clinical parameters in HCC patients, there was a significant association between+1234C/T SNP; namely TT genotype and each of the hepatic focal lesions᾽ number, size and the patients᾽ higher Okuda and BCLC stages. No association could be detected between TLR3 SNP and the age, sex, Child-Pugh grades, MELD score or AFP of the studied HCC cases. Conclusion: TLR3 gene SN P +1234C/T could be a novel risk factor for the HCV-related HCC among the Egyptian population.

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