Seawater-irrigation effects on growth, ion concentration, and photosynthesis of transgenic poplar overexpressing the Na+/H+ antiporter AtNHX1

2011 ◽  
Vol 174 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Chaoqiang Jiang ◽  
Qingsong Zheng ◽  
Zhaopu Liu ◽  
Ling Liu ◽  
Gengmao Zhao ◽  
...  
Keyword(s):  
Ion Concentration ◽  
Transgenic Poplar ◽  
Seawater Irrigation ◽  
Irrigation Effects

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

Confocal imaging of calcium in intact ventricular muscle provides a new view of excitation-contraction coupling

1996 ◽  
Vol 54 ◽  
pp. 738-739
Author(s):  
W.G. Wier
Keyword(s):  
Local Control ◽  
Cardiac Tissue ◽  
Cell Function ◽  
Isolated Heart ◽  
Cardiac Cells ◽  
Confocal Imaging ◽  
Ion Concentration ◽  
Heart Cell ◽  
Free Calcium ◽  
Heart Cells

A fundamentally new understanding of cardiac excitation-contraction (E-C) coupling is being developed from recent experimental work using confocal microscopy of single isolated heart cells. In particular, the transient change in intracellular free calcium ion concentration ([Ca2+]i transient) that activates muscle contraction is now viewed as resulting from the spatial and temporal summation of small (∼ 8 μm3), subcellular, stereotyped ‘local [Ca2+]i-transients' or, as they have been called, ‘calcium sparks'. This new understanding may be called ‘local control of E-C coupling'. The relevance to normal heart cell function of ‘local control, theory and the recent confocal data on spontaneous Ca2+ ‘sparks', and on electrically evoked local [Ca2+]i-transients has been unknown however, because the previous studies were all conducted on slack, internally perfused, single, enzymatically dissociated cardiac cells, at room temperature, usually with Cs+ replacing K+, and often in the presence of Ca2-channel blockers. The present work was undertaken to establish whether or not the concepts derived from these studies are in fact relevant to normal cardiac tissue under physiological conditions, by attempting to record local [Ca2+]i-transients, sparks (and Ca2+ waves) in intact, multi-cellular cardiac tissue.

INVESTIGATION OF BEE BREAD AND DEVELOPMENT OF ITS DOSAGE FORMS

2014 ◽  
Vol 21 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Ilze Barene ◽  
Irena Daberte ◽  
Sanita Siksna
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Potato Starch ◽  
Microscopic Analysis ◽  
Stability Study ◽  
Ion Concentration ◽  
Wet Granulation ◽  
Hydrogen Ion Concentration ◽  
Bee Bread ◽  
Layer Chromatography

The aim of the study. The objective of this study was to investigate microscopic, physical and chemical properties of bee bread collected in three regions of Latvia in order to compare the quality and to investigate the possibility of producing granules containing bee bread. Material and methods. Microscopic analysis of bee bread samples was performed. Plant herbaria, special literature and internet sources were used for identification of pollen. Thin layer chromatography was used for identification of carotenoids and flavonoids. Granules were prepared by wet granulation method. Lactose, calcium lactate, calcium carbonate, potato starch and purified water were used as excipients. Appearance, loss on drying, pH of aqueous solution and content of carotenes were estimated. Results. Microscopic analysis showed mostly native pollen identified as willow pollen. Beta-carotene identified and 2 carotenoids found by thin layer chromatography. Two zones of flavonoids found on chromatograms at day light and 6 zones at ultra violet light. The comparison of bee bread samples of 3 regions of Latvia showed insignificant differences in appearance and consistency, hydrogen ion concentration 3.93–4.23, loss on drying 7.72–11.07 %; content of carotenes calculated to bcarotene 6.77–9.35 mg%. Stability study of bee bread samples showed greater changes after storage at 40ºC temperature. All compositions of granules showed appropriate appearance and flowability. Quality of granules: loss on drying 5.48–13.5%, content of carotenes calculated to b-carotene 5.77–6.75 mg%. Conclusions. Pollen of willow can be considered as an indicator of the origin of bee bread in Latvia. Bee bread samples of three regions of Latvia have insignificant differences in physical, chemical parameters.

Fluctuations of Annual Precipitation and Water Resources Quality in Ukraine

2016 ◽  
Vol 10 (4s) ◽  
pp. 621-629
Author(s):  
Valentina Pidlisnyuk ◽  
◽  
John Harrington JR ◽  
Yulia Melnyk ◽  
Yuliya Vystavna ◽  
...  
Keyword(s):  
Water Resources ◽  
Surface Waters ◽  
Precipitation Amount ◽  
Ion Concentration ◽  
Annual Precipitation ◽  
Resource Quality ◽  
Phosphate Ion ◽  
Regional Trends

The article focuses on examining the influence of fluctuations in annual precipitation amount on the quality of surface waters. Water quality was estimated with data on BOD, COD and phosphate–ion concentration within five selected regions of Ukraine. Analysis of the precipitation data (1991 – 2010) showed different regional trends. Using the statistics, determination of the interconnection between precipitation amount and water resources quality were done. The obtained regularities and associated uncertainties can be used for prediction of changes in water resource quality and as a guide for future adaptation to possible climate change.

Hydro-geochemical properties with respect to landuse in the southernmost river of Kerala

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Badusha M. ◽  
Santhosh S
Keyword(s):  
Organic Carbon ◽  
Drainage Basin ◽  
Anthropogenic Activities ◽  
Oxygen Demand ◽  
Ion Concentration ◽  
Total Hardness ◽  
Hydrogen Ion Concentration ◽  
Geochemical Properties ◽  
Quality Of Water

The hydro geochemical features of Neyyar River for a period of one year from May 2015 to April 2016 were analyzed. Six sampling sites were fixed considering physiography and present landuse pattern of the river basin. The residents in the drainage basin are primarily responsible for framing a better landuse and thereby maintain a good water and sediment regime. Geospatial pattern of the present landuse of the study area indicated that the sustainability of this river ecosystem is in danger due to unscientific landuse practices, which is reflected in the river quality as well. The parameters such as hydrogen ion concentration, electrical conductivity, chloride, Biological Oxygen Demand, total hardness and sulphate of river water and Organic Carbon of river bed sediments were analyzed in this study. The overall analysis shows that the highland areas are characterized by better quality of water together with low organic carbon, which is mainly due to better landuse and minimal reclamation. The midland and lowland areas are characterized by poor quality of water with high organic carbon, which is due to high anthropogenic activities and maximum pollutants associated with the region together with the alteration in landuse from a traditional eco-friendly pattern to a severely polluted current pattern.

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