scholarly journals 1 H NMR metabolomics study of age profiling in children

2009 ◽  
Vol 22 (8) ◽  
pp. 826-833 ◽  
Author(s):  
Haiwei Gu ◽  
Zhengzheng Pan ◽  
Bowei Xi ◽  
Bryan E. Hainline ◽  
Narasimhamurthy Shanaiah ◽  
...  
Keyword(s):  
Nmr Metabolomics ◽  
H Nmr ◽  
Metabolomics Study

scholarly journals Nor-kurarinone Characteristic of Chinese Sophora flavescens

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Ryuichiro Suzuki ◽  
Utsumi Takahiro ◽  
Risa Takao ◽  
Yoshiaki Shirataki
Keyword(s):  
Nmr Spectra ◽  
Hplc Analysis ◽  
Sophora Flavescens ◽  
Nmr Metabolomics ◽  
Root Extracts ◽  
H Nmr ◽  
Plot Analysis ◽  
Metabolomics Study ◽  
Loading Plot ◽  
Nmr Signals

The root of Sophora flavescens is used as a Chinese crude drug for treating various disorders. S. flavescens is grown in Japan, China and Korea. We previously used Principle Component Analysis (PCA) to demonstrate that 1H NMR spectra of root extracts of S. flavescens can be used to identify the country of origin of the sample (Japan or China). In the present study we identified a compound characteristic of Chinese S. flavescens using NMR metabolomics. Loading plot analysis of the PCA results and an NMR metabolomics study identified NMR signals from a compound identified as nor-kurarinone and characteristic of Chinese S. flavescens grown in Hangzhou. This compound was confirmed by HPLC analysis to be a distinctive marker for Chinese S. flavescens.

scholarly journals Putative imbalanced amino acid metabolism in rainbow trout long term fed a plant-based diet as revealed by 1H-NMR metabolomics

2021 ◽  
Vol 10 ◽  
Author(s):  
Catherine Deborde ◽  
Blandine Madji Hounoum ◽  
Annick Moing ◽  
Mickaël Maucourt ◽  
Daniel Jacob ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Proton Nuclear Magnetic Resonance ◽  
Free Form ◽  
Metabolomic Analysis ◽  
Nmr Metabolomics ◽  
H Nmr

Abstract The long-term effect of a plant (P)-based diet was assessed by proton nuclear magnetic resonance (1H-NMR) metabolomics in rainbow trout fed a marine fish meal (FM)–fish oil (FO) diet (M), a P-based diet and a control commercial-like diet (C) starting with the first feeding. Growth performances were not heavily altered by long-term feeding on the P-based diet. An 1H-NMR metabolomic analysis of the feed revealed significantly different soluble chemical compound profiles between the diets. A set of soluble chemical compounds was found to be specific either to the P-based diet or to the M diet. Pterin, a biomarker of plant feedstuffs, was identified both in the P-based diet and in the plasma of fish fed the P-based diet. 1H-NMR metabolomic analysis on fish plasma and liver and muscle tissues at 6 and 48 h post feeding revealed significantly different profiles between the P-based diet and the M diet, while the C diet showed intermediate results. A higher amino acid content was found in the plasma of fish fed the P-based diet compared with the M diet after 48 h, suggesting either a delayed delivery of the amino acids or a lower amino acid utilisation in the P-based diet. This was associated with an accumulation of essential amino acids and the depletion of glutamine in the muscle, together with an accumulation of choline in the liver. Combined with an anticipated absorption of methionine and lysine supplemented in free form, the present results suggest an imbalanced essential amino acid supply for protein metabolism in the muscle and for specific functions of the liver.

An NMR-based metabolomics study on sea anemones Exaiptasia diaphana (Rapp, 1829) with atrazine exposure

2021 ◽  
Author(s):  
Lin Jiang ◽  
Hunter Sullivan ◽  
Cole Seligman ◽  
Sandra Gilchrist ◽  
Bo Wang
Keyword(s):  
Sea Anemones ◽  
Nmr Metabolomics ◽  
Metabolomics Study ◽  
Early Biomarker

NMR metabolomics was performed on E. diaphana exposed to atrazine, and glutamate was discovered as a potential early biomarker.

1 H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

2020 ◽  
Vol 31 (4) ◽  
pp. 480-487 ◽  
Author(s):  
Elvia Becerra‐Martínez ◽  
Yesenia Pacheco‐Hernández ◽  
Edmundo Lozoya‐Gloria ◽  
Martha G. Betancourt‐Jiménez ◽  
Diego Hidalgo‐Martínez ◽  
...  
Keyword(s):  
Tobacco Plants ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Sesquiterpene Cyclase

scholarly journals 1H NMR metabolomics study of metastatic melanoma in C57BL/6J mouse spleen

Metabolomics ◽  
2014 ◽  
Vol 10 (6) ◽  
pp. 1129-1144 ◽  
Author(s):  
Xuan Wang ◽  
Mary Hu ◽  
Ju Feng ◽  
Maili Liu ◽  
Jian Zhi Hu
Keyword(s):  
Metastatic Melanoma ◽  
1H Nmr ◽  
Mouse Spleen ◽  
Nmr Metabolomics ◽  
Metabolomics Study

NMR metabolic profiling of lipopolysaccharide-induced mice sepsis and the treatment effects of berberine

RSC Advances ◽  
2016 ◽  
Vol 6 (53) ◽  
pp. 47474-47485 ◽  
Author(s):  
Pei Li ◽  
Shanting Liao ◽  
Junsong Wang ◽  
Dingqiao Xu ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Treatment Effects ◽  
Nmr Metabolomics ◽  
H Nmr

1H NMR metabolomics to study lipopolysaccharide-induced mice sepsis and the treatment effects of berberine.

Postprandial response on fatty meal is affected by sea buckthorn (Hippophaë rhamnoides) supplementation: NMR metabolomics study

2014 ◽  
Vol 58 ◽  
pp. 23-34 ◽  
Author(s):  
Anni Lindstedt ◽  
Riikka Järvinen ◽  
Jari Sinkkonen ◽  
Henna-Maria Lehtonen ◽  
Gonçalo Graça ◽  
...  
Keyword(s):  
Sea Buckthorn ◽  
Hippophae Rhamnoides ◽  
Fatty Meal ◽  
Nmr Metabolomics ◽  
Hippophaë Rhamnoides ◽  
Postprandial Response ◽  
Metabolomics Study

scholarly journals 1H-NMR Metabolomics Analysis of The Intervention Effects of Ruangan Xiaoji Decoction on CCl4 Induced Liver Fibrosis in Rats

2020 ◽  
Author(s):  
Weiliang Zhu ◽  
Guoqing Wu ◽  
Wenrui Zhu ◽  
Tianwen Zhao ◽  
Hong-Jiang Zhang ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Tissue ◽  
Acid Metabolism ◽  
Clinical Chemistry ◽  
Tca Cycle ◽  
Control Group ◽  
Obvious Effect ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Endogenous Metabolites

Abstract Background: Liver fibrosis is a common consequence of chronic liver diseases resulting from multiple etiologies. Early clinical application shows that Ruangan Xiaoji Decoction (RGXJD) has a very obvious effect on the treatment of liver fibrosis. However, the mechanism of RGXJD cures liver fibrosis requires further elucidation.Methods: In this work, the therapeutic effect of RGXJD on CCl4-induced liver fibroses serum and liver tissue metabolite changes in rat was analyzed by 1H-NMR metabolomics. Meanwhile, histopathology examinations and serum clinical chemistry analysis verified the experimental results of metabolomics.Results: RGXJD treatment could reverse the increase in ALT and AST induced by CCl4 and attenuate the pathological changes in liver tissue. In the 1H-NMR metabolomic analysis, PLS-DA score plots demonstrated that the serum and liver tissue metabolic profiles in rats of the RGXJD groups were similar those of the control group, yet remarkably apart from the CCl4 group. The mechanism may be related to the endogenous metabolites including energy metabolism, amino acid metabolism, TCA cycle and purine metabolism in rats. Correlation analysis were then performed to further confirm the metabolites involved in Isoleucine, Tyrosine, UDP-Glucose, Glutathione and Leucine, etc.Conclusions: These findings may provided new insights into the mechanism of the hepatoprotection of RGXJD.

scholarly journals Metabolic profiling of CSF from people suffering from Sporadic and LRRK2 Parkinson’s disease: a pilot study.

2020 ◽  
Author(s):  
Ali Yilmaz ◽  
Zafer Ugur ◽  
Ilyas Ustun ◽  
Ray O. Bahado-Singh ◽  
Michael Maddens ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Machine Learning Techniques ◽  
Bile Acid Metabolism ◽  
Holistic View ◽  
H Nmr ◽  
Biochemical Pathways ◽  
Metabolomics Study ◽  
And Gender ◽  
The Brain

Abstract Background: CSF from unique groups of Parkinson’s disease (PD) patients were biochemically profiled to identify previously unreported metabolic pathways linked to PD pathogenesis and novel biochemical biomarkers of the disease were characterized. Methods: Utilizing both 1H NMR and DI-LC-MS/MS we quantitatively profiled CSF from patients with sporadic PD (n=20), those who are genetically predisposed (LRRK2) to the disease (n=20) and compared them with age,- and gender-matched controls (n=20). Further, we systematically evaluated the utility of several machine learning techniques for the diagnosis of PD.Results: 1H NMR and mass spectrometry-based metabolomics, in combination with bioinformatic analyses, provided useful information highlighting previously unreported biochemical pathways and CSF based biomarkers associated with both sporadic PD (sPD) and LRRK2 PD. Results of this metabolomics study further support our group’s previous findings identifying Bile Acid metabolism as one of the major aberrant biochemical pathways in PD patients.Conclusion: This study demonstrates that a combination of two complimentary techniques can provide a much more holistic view of the CSF metabolome and by association, the brain metabolome. Future studies for the prediction of those at risk of developing PD should investigate the clinical utility of these CSF based biomarkers in more accessible biomatrices. Further, it is essential that we determine if the biochemical pathways highlighted here are recapitulated in the brain of PD patients with the aim of identifying potential therapeutic targets.

Sign in / Sign up

Export Citation Format

Share Document