scholarly journals 1H-NMR Metabolomics Analysis of The Intervention Effects of Ruangan Xiaoji Decoction on CCl4 Induced Liver Fibrosis in Rats

2020 ◽  
Author(s):  
Weiliang Zhu ◽  
Guoqing Wu ◽  
Wenrui Zhu ◽  
Tianwen Zhao ◽  
Hong-Jiang Zhang ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Tissue ◽  
Acid Metabolism ◽  
Clinical Chemistry ◽  
Tca Cycle ◽  
Control Group ◽  
Obvious Effect ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Endogenous Metabolites

Abstract Background: Liver fibrosis is a common consequence of chronic liver diseases resulting from multiple etiologies. Early clinical application shows that Ruangan Xiaoji Decoction (RGXJD) has a very obvious effect on the treatment of liver fibrosis. However, the mechanism of RGXJD cures liver fibrosis requires further elucidation.Methods: In this work, the therapeutic effect of RGXJD on CCl4-induced liver fibroses serum and liver tissue metabolite changes in rat was analyzed by 1H-NMR metabolomics. Meanwhile, histopathology examinations and serum clinical chemistry analysis verified the experimental results of metabolomics.Results: RGXJD treatment could reverse the increase in ALT and AST induced by CCl4 and attenuate the pathological changes in liver tissue. In the 1H-NMR metabolomic analysis, PLS-DA score plots demonstrated that the serum and liver tissue metabolic profiles in rats of the RGXJD groups were similar those of the control group, yet remarkably apart from the CCl4 group. The mechanism may be related to the endogenous metabolites including energy metabolism, amino acid metabolism, TCA cycle and purine metabolism in rats. Correlation analysis were then performed to further confirm the metabolites involved in Isoleucine, Tyrosine, UDP-Glucose, Glutathione and Leucine, etc.Conclusions: These findings may provided new insights into the mechanism of the hepatoprotection of RGXJD.

Experimental Study Oviductus ranae for the Change of α-SMA and TGF-β in Hepatic Fibrosis Rat Liver

2014 ◽  
Vol 912-914 ◽  
pp. 1940-1943
Author(s):  
Yan Li ◽  
Xiao Ou Li ◽  
Feng Hao ◽  
Lei Zhang ◽  
Lei Liu ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Fibrosis ◽  
Rat Liver ◽  
Liver Tissue ◽  
Female Rats ◽  
Control Group ◽  
Group Model ◽  
Model Group ◽  
Model Control ◽  
Oviductus Ranae

To evaluate the control effect of Oviductus ranae on liver fibrosis in rats, and the change of TGF-β and α-SMA in liver of. To explore the mechanism of Oviductus ranae decoction on liver fibrosis. Methods Wistar female rats were randomly divided into a blank control group, model control group, colchicines group, Oviductus ranae group. Using the CCl4composite approach to make the rat modle. The course of treat-mart was 12 weeks.After treatment,All the rats was killed,and the materials and blood was taken,and to detect biochemical test of liver function after eight weeks. Investigating the variation of liver histology. Meanwhile detecting protein expression of TGF-β and α-SMA and by immunehistochemical method.Result The general condition of rats in all treatment groups are worse than the blank group,but better than the model group. And the rats in the model group were all occurred in liver fibrosis,and liver fibrosis is the most serious.In a normal rat liver tissue of TGF-β and α-SMA were significantly lower in model group and each treatment group, and there were significant differences, and the TGF-β and α-SMA in expression of liver tissue in model rats of TGF-β and α-SMA the highest. Conclusion: Oviductus ranae can effectively improve liver fibrosis rats induced by CC14liver function.Oviductus ranae can reduce the expression of TGF-β1in liver tissue of hepatic fibrosis rats induced by CCl4in. This may be one of the mechanisms of Oviductus ranae in prevention and treatment of liver fibrosis. Even though both increased expression of TGF-β and α-SMA expression, is able to determine TGF-β and α-SMA for the intervention of liver TGF-β signal transduction pathway in liver fibrosis.

Regulation on BCAAs catabolism pathway plays the key role in cyclophosphamide-induced leucopenia BALB/c mice after the treatment of a typical Traditional Chinese Medicine of Lvjiao Buxue Granules

2020 ◽  
Author(s):  
Jun-sheng Tian ◽  
Hui-liang Zhao ◽  
Qi Wang ◽  
Huan Xiang ◽  
Xiang-ping Xu ◽  
...  
Keyword(s):  
Cancer Chemotherapy ◽  
Blood Cells ◽  
White Blood Cells ◽  
Degradation Pathway ◽  
Integrated Analysis ◽  
Nmr Metabolomics ◽  
Biological Targets ◽  
H Nmr ◽  
Branched Chain ◽  
Endogenous Metabolites

Abstract Background: Cyclophosphamide is a common tumor chemotherapy drug used to treat various cancers, but the resulting immunosuppression leads to leukopenia, which is a serious limiting factor in clinical application. Therefore, the introduction of immunomodulators as adjuvant therapy may help to reduce the hematological side effects of cyclophosphamide. Lvjiao Buxue Granules has been widely used in clinical treatment of gynecological diseases such as anemia and irregular menstruation, and recently, it has been found to increase the role of white blood cells, but its mechanism of action is still unclear. In this research, we applied the 1H-NMR metabolomics approach to characterize metabolites in cyclophosphamide-induced leucopenia mice spleen, so as to fully understand the metabolic processes of leucopenia and improve the leukocyte function of Lvjiao Buxue Granules.Methods: Cyclophosphamide was used to establish the leucopenia mice with cancer chemotherapy and the content of white blood cells, red blood cells, hemoglobin, platelets, and other routine blood indexes were measured. The changes of endogenous metabolites in spleen analyzed by 1H-NMR metabolomics technique were investigated the regulation effect of LBG in mice with leukopenia. Afterward, the chemical components-targets-differential metabolites network of Lvjiao Buxue Granules was constructed by the use of biological targets network, thus leucopenia-relevant metabolism pathways were dissected. Results: The blood routine parameters and organ indexes levels of leucopenia mice with cancer chemotherapy were improved by Lvjiao Buxue Granules. The metabolomic study revealed that 15 endogenous metabolites in mice spleen were considered as potential biomarkers of Lvjiao Buxue Granules for its protective effect. Integrated analysis of metabolomics and biological targets network indicated that Lvjiao Buxue Granules exerted the leukocyte elevation activity by inhibiting the branched-chain amino acids (BCAAs) degradation pathway and increasing the levels of valine, leucine and isoleucine. Conclusion: Lvjiao Buxue Granules exert obvious efficacy on the mice model of leucopenia, which could be improved by regulating the branched-chain amino acid degradation pathway and the levels of valine, leucine and isoleucine.

scholarly journals Hepatoprotective effect of Moringa oleifera extract on TNF-α and TGF-β expression in acetaminophen-induced liver fibrosis in rats

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Omnia Aly ◽  
Dalia M. Abouelfadl ◽  
Olfat G. Shaker ◽  
Gehan A. Hegazy ◽  
Ahmed M. Fayez ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Tissue ◽  
Moringa Oleifera ◽  
Hepatoprotective Effect ◽  
Tissue Homogenate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Group I ◽  
Tnf Α

Abstract Background It has been reported that Moringa oleifera (MO) has different medicinal properties. The aim of this study was to evaluate the hepatoprotective role of Moringa oleifera extract on acetaminophen-induced liver fibrosis in albino rats on a biochemical and histological basis. Forty male albino rats were divided into four groups: group I (control group), healthy rates; group II (acetaminophen group), rates received acetaminophen for induction of liver fibrosis; group III (treated group), liver fibrosis of rates treated with Moringa oleifera extract; and group IV (prophylactic group), rates treated with Moringa oleifera extract before and after induction of liver fibrosis. Serum liver function parameters were quantified using a spectrophotometer, while tumor necrosis factor α (TNF-α) and transformed growth factor beta (TGF- β) in liver tissue homogenate by means of enzyme-linked immunosorbent assay (ELISA), and expression of liver tissue TNF-α and TGF-genes was measured by real-time PCR after extraction and purification. Hepatic tissue was also evaluated under a microscope for histopathological changes. Results Our results showed a significant decrease in liver enzymes, TNF-α, and TGF-β in the treated and prophylactic groups compared to the acetaminophen group, and our biochemical data were consistent with the histopathological findings confirming the hepatoprotective effect of Moringa oleifera extract. Conclusions Biochemical parameters and histopathology results provide evidence that Moringa oleifera ethanolic extract has a great potential to prevent and improve liver damage due to its protective activity.

scholarly journals QGQS Granule in SHR Serum Metabonomics Study Based on Tools of UPLC-Q-TOF and Renin-Angiotensin-Aldosterone System Form Protein Profilin-1

2017 ◽  
Vol 2017 ◽  
pp. 1-13
Author(s):  
Ke Li ◽  
Caicai Li ◽  
Jialong Wang ◽  
Hanming Cui ◽  
Yu Dong ◽  
...  
Keyword(s):  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Control Group ◽  
Ang Ii ◽  
Sample Analysis ◽  
Model Group ◽  
Tail Artery ◽  
Endogenous Metabolites ◽  
Tof Ms

QGQS granule is effective for the therapeutic of hypertension in clinic. The aim of this research is to observe the antihypertension effect of QGQS granule on SHR and explain the mechanism of its lowering blood pressure. 30 SHR were selected as model group, captopril group, and QGQS group, 10 WKYr were used as control group, and RBP were measured on tail artery consciously. And all the serum sample analysis was carried out on UPLC-TOF-MS system to determine endogenous metabolites and to find the metabonomics pathways. Meanwhile, ELISA kits for the determination pharmacological indexes of PRA, AngI, AngII, and ALD were used for pathway confirmatory; WB for determination of profilin-1 protein expression was conducted for Ang II pathway analysis as well. It is demonstrated that QGQS granule has an excellent therapeutic effect on antihypertension, which exerts effect mainly on metabonomics pathway by regulating glycerophospholipid, sphingolipid, and arachidonic acid metabolism, and it could inhibit the overexpression of the profilin-1 protein. We can come to a conclusion that RAAS should be responsible mainly for the metabonomics pathway of QGQS granule on antihypertension, and it plays a very important role in protein of profilin-1 inhibition.

scholarly journals Metabolic Profiling Analysis of the Alleviation Effect of the Fractions of Niuhuang Jiedu Tablet on Realgar Induced Toxicity in Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Wenfeng Xu ◽  
Yuehu Pei ◽  
Shuo Xu ◽  
Haifeng Wang ◽  
Pengfei Jin
Keyword(s):  
Histopathological Examination ◽  
Clinical Chemistry ◽  
Control Group ◽  
Metabolic Profiles ◽  
Acute Tonsillitis ◽  
H Nmr ◽  
Liver And Kidney ◽  
Metabolic Profiling Analysis ◽  
Pattern Recognition Analysis ◽  
Urine And Serum

Niuhuang Jiedu Tablet (NJT) is a classical formula in treating acute tonsillitis, pharyngitis, and so on. In the formula, significant level of Realgar as a potentially toxic element is contained. Our previous experiments revealed that it was less toxic for combined Realgar in NJT. However, the active fraction of this prescription with toxicity alleviation effect on Realgar was still obscure. NJT was divided into five different polar fractions (NJT-PET, NJT-25, NJT-50, NJT-75, and NJT-95), and we explored the toxicity alleviation effect on Realgar. Based on 1H NMR spectra of urine and serum from rats, PCA and PLS-DA were performed to identify different metabolic profiles. Liver and kidney histopathology examinations and serum clinical chemistry analysis were also performed. With pattern recognition analysis of metabolites in urine and serum, Realgar group showed a clear separation from control group, while the metabolic profiles of NJT-PET, NJT-25, NJT-50, and NJT-95 groups were similar to Realgar group, and the metabolic profiles of NJT and NJT-75 groups were very close to control group. Statistics results were confirmed by the histopathological examination and biochemical assay. The present work indicated that 75% EtOH fraction of NJT was the most valid fraction with the toxicity alleviation effect on Realgar.

scholarly journals Urine Organic Acids Can Determine the Metabolic Effects of Traditional Korean Diet, a Cardiometabolic Diet

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 14-14
Author(s):  
Sukyung Chun ◽  
Phil-Kyung Shin ◽  
Myung Sunny Kim ◽  
Min Jung Kim ◽  
Hae-Jeung Lee ◽  
...  
Keyword(s):  
Amino Acid ◽  
Organic Acids ◽  
Acid Metabolism ◽  
Tca Cycle ◽  
Diet Group ◽  
Metabolic Effects ◽  
Control Group ◽  
Branched Chain Amino Acid ◽  
Branched Chain ◽  
Urine Organic Acids

Abstract Objectives Urine organic acids are water-soluble chemical compounds excreted in the urine that are intermediates in many metabolic pathways. Because excess metabolites are excreted in the urine, they may reflect the metabolic status. We investigated whether urine organic acids can determine the metabolic influence of traditional Korean diet (K-diet), a cardiometabolic diet. Methods Fifty two healthy premenopausal women were recruited into a 2 × 2 crossover study with two different diets, K diet and control diet (Westernized current Korean diet). Each diet was provided alternately to all subjects for 1 month with 1 month washout period. Blood and urine samples were collected with anthropometric measurements. Sixty five urine organic acids were measured by LC-MS/MS. Results In the K-diet group, the mean difference of triglyceride (−6.58 ± 4.80 vs −26.35 ± 6.05 mg/dL, P = 0.012), total cholesterol (−12.77 ± 2.78 vs −30.02 ± 2.65 mg/dL, P < 0.001) and LDL- cholesterol (−5.52 ± 2.30 vs −15.88 ± 2.29 mg/dL, P = 0.002) were significantly lower compared to the control group. Body weight and body mass index were also lowered in the K-diet group. Among 65 urine organic acids, TCA cycle associated α-ketoglutarate (21.73 ± 1.27 vs 19.05 ± 1.15 ug/mg creatinine, P = 0.008), malate (1.61 ± 0.10 vs 1.34 ± 0.09, P = 0.008) and isocitrate (22.90 ± 0.68 vs 21.27 ± 0.54, P = 0.014) were lowered in the K-diet group, while citrate (249.16 ± 16.96 vs 324.42 ± 21.18, P = 0.001) and furmarate (0.51 ± 0.03 vs 0.65 ± 0.06, P = 0.029) were elevated in the control group. α-Keto-β-methylvalerate (1.46 ± 0.09 vs 1.09 ± 0.07, P = 0.001), α-ketoisovalerate (0.22 ± 0.02 vs 0.16 ± 0.02, P = 0.012) and methylmalonate (1.30 ± 0.06 vs 1.00 ± 0.06, P < 0.001), which are associated with branched chain amino acid metabolism, were decreased in the K-diet group. Adipate (0.90 ± 0.07 vs 0.65 ± 0.04, P < 0.001) and α-ketoisocaproate (0.46 ± 0.02 vs 0.38 ± 0.02, P = 0.002), which are associated with fatty acid metabolism, were also decreased in the K-diet group. Conclusions K-diet improves clinical parameters and alters urine organic acids associated with TCA cycle and metabolisms of fatty acids and branched-chain amino acid, suggesting that urine organic acids can be useful endpoints to determine the metabolic effects of K-diet and even other diets. Funding Sources Korea Food Research Institute.

Withdrawn: Hepatoprotective effects of Tribulus terrestris L. (Zygophyllales: Zygophyllaceae) hydro-alcholic extract on non-alcoholic fatty liver-induced rats

2016 ◽  
Vol 3 (5) ◽  
pp. 143
Author(s):  
Fatemeh Almasi ◽  
Mozafar Khazaei ◽  
Shima Chehrei ◽  
Ali Ghanbari
Keyword(s):  
Fatty Liver ◽  
Liver Tissue ◽  
Serum Lipid ◽  
Histopathological Examination ◽  
Serum Levels ◽  
Lipid Profiles ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Protective Effects ◽  
Alcoholic Fatty Liver

Non-alcoholic fatty liver induces many complications to the liver tissue and also serum related parameters. Medicinal plants are the safe therapeutic strategy for the treatment of diseases. In this regards, the present study was conducted to evaluate the effect of Tribulus terrestris L. (Zygophyllales: Zygophyllaceae) extract on non-alcoholic fatty liver in rats. In this experimental study, thirty male Wistar rats were divided into five groups (n = 6). Animals in experimental groups were received high fructose diet (70%) (HDF) daily alone or in combined with daily intraperitoneal injection of 500, 700 and 1,000 mg/kg extract of T. terrestris. Control group of rats was feed with standard chow. The serum levels of biomarkers of liver and serum lipid profiles were assessed, also histopathological examination of liver tissue done. Data were analyzed using One-way ANOVA method followed by Tukey’s post-hoc multiple comparison test and P < 0.05 was considered statistically significant. There were significant improvements for biomarkers of liver tissue (P < 0.05) and serum lipid profiles (P < 0.01) in the HFD-fed rats that were treated with T. terrestris extract compare to HFD-fed group. In addition, accumulation of lipids in hepatocytes was significantly reduced in the HFD-fed + extract administrated groups in comparison to HFD-fed rats (P < 0.01). T. terrestris extract has protective effects against non-alcoholic fatty liver by changing biomarkers of liver tissue, serum lipid profiles and histopathological anomalies of liver tissue, to normal range.

Effect of Chinese Herbal Monomer Hairy Calycosin on Nonalcoholic Fatty Liver Rats and its Mechanism

2019 ◽  
Vol 22 (3) ◽  
pp. 194-200 ◽  
Author(s):  
Xiang Liu ◽  
Zhi-Hong Xie ◽  
Chen-Yuan Liu ◽  
Ying Zhang
Keyword(s):  
Fatty Liver ◽  
Liver Tissue ◽  
Liver Homogenate ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Alcoholic Fatty Liver ◽  
Nonalcoholic Fatty Liver ◽  
Chinese Herbal ◽  
Tnf Α ◽  
Model Control

Background: Chinese herbal monomer hairy Calycosin is a flavonoid extracted from Radix astragali. Aims and Scope: The aim of the research was to investigate the effect and mechanism of Hairy Calycosin on Non-Alcoholic Fatty Liver Dieases (NAFLD) in rats. Materials and Methods: 60 rats were randomly divided into 6 groups, then NAFLD rat models were prepared and treated with different doses of Hairy Calycosin (0.5, 1.0, 2.0 mg/kg) or Kathyle relatively. Results: Both 1.0 mg/kg and 2.0 mg/kg Hairy Calycosin treatment could significantly increase the serum Superoxide Dismutase (SOD) content of the model rats and reduce the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), Free Fatty Acid (FFA), IL-6, tumor necrosis factor-alpha (TNF-α) and liver homogenate malondialdehyde (MDA), while 2.0 mg/kg Hairy Calycosin can down-regulate liver tissue cytochrome p450 2E1 (CYP2E1). In the electron microscope, compared with the model control group, the mitochondrial swelling in the hepatocytes of Hairy Calycosin (1.0, 2.0 mg/kg) treatment group was significantly reduced, the ridge on the inner membrane of mitochondria increased, and the lipid droplets became much smaller. Conclusion: Hairy Calycosin can effectively control the lipid peroxidation in liver tissues of rats with NAFLD, and reduce the levels of serum TNF-α, IL-6, MDA and FFA, effectively improve the steatosis and inflammation of liver tissue, and down-regulate the expression of CYP2E1, inhibit apoptosis of hepatocytes.

scholarly journals Effect of Huangqin Tang on Urine Metabolic Profile in Rats with Ulcerative Colitis Based on UPLC-Q-Exactive Orbitrap MS

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Dunfang Wang ◽  
Xuran Ma ◽  
Shanshan Guo ◽  
Yanli Wang ◽  
Tao Li ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Fatty Acids ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Unsaturated Fatty Acids ◽  
Tricarboxylic Acid Cycle ◽  
Tca Cycle ◽  
Q Exactive ◽  
Therapeutic Mechanisms

As a classic prescription, Huangqin Tang (HQT) has been widely applied to treat ulcerative colitis (UC), although its pharmacological mechanisms are not clear. In this study, urine metabolomics was first analysed to explore the therapeutic mechanisms of HQT in UC rats induced by TNBS. We identified 28 potential biomarkers affected by HQT that might cause changes in urine metabolism in UC rats, mapped the network of metabolic pathways, and revealed how HQT affects metabolism of UC rats. The results showed that UC affects amino acid metabolism and biosynthesis of unsaturated fatty acids and impairs the tricarboxylic acid cycle (TCA cycle). UC induced inflammatory and gastrointestinal reactions by inhibiting the transport of fatty acids and disrupting amino acid metabolism. HQT plays key roles via regulating the level of biomarkers in the metabolism of amino acids, lipids, and so on, normalizing metabolic disorders. In addition, histopathology and other bioinformatics analysis further confirm that HQT altered UC rat physiology and pathology, ultimately affecting metabolic function of UC rats.

scholarly journals Putative imbalanced amino acid metabolism in rainbow trout long term fed a plant-based diet as revealed by 1H-NMR metabolomics

2021 ◽  
Vol 10 ◽  
Author(s):  
Catherine Deborde ◽  
Blandine Madji Hounoum ◽  
Annick Moing ◽  
Mickaël Maucourt ◽  
Daniel Jacob ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Proton Nuclear Magnetic Resonance ◽  
Free Form ◽  
Metabolomic Analysis ◽  
Nmr Metabolomics ◽  
H Nmr

Abstract The long-term effect of a plant (P)-based diet was assessed by proton nuclear magnetic resonance (1H-NMR) metabolomics in rainbow trout fed a marine fish meal (FM)–fish oil (FO) diet (M), a P-based diet and a control commercial-like diet (C) starting with the first feeding. Growth performances were not heavily altered by long-term feeding on the P-based diet. An 1H-NMR metabolomic analysis of the feed revealed significantly different soluble chemical compound profiles between the diets. A set of soluble chemical compounds was found to be specific either to the P-based diet or to the M diet. Pterin, a biomarker of plant feedstuffs, was identified both in the P-based diet and in the plasma of fish fed the P-based diet. 1H-NMR metabolomic analysis on fish plasma and liver and muscle tissues at 6 and 48 h post feeding revealed significantly different profiles between the P-based diet and the M diet, while the C diet showed intermediate results. A higher amino acid content was found in the plasma of fish fed the P-based diet compared with the M diet after 48 h, suggesting either a delayed delivery of the amino acids or a lower amino acid utilisation in the P-based diet. This was associated with an accumulation of essential amino acids and the depletion of glutamine in the muscle, together with an accumulation of choline in the liver. Combined with an anticipated absorption of methionine and lysine supplemented in free form, the present results suggest an imbalanced essential amino acid supply for protein metabolism in the muscle and for specific functions of the liver.

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