1 H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

2020 ◽  
Vol 31 (4) ◽  
pp. 480-487 ◽  
Author(s):  
Elvia Becerra‐Martínez ◽  
Yesenia Pacheco‐Hernández ◽  
Edmundo Lozoya‐Gloria ◽  
Martha G. Betancourt‐Jiménez ◽  
Diego Hidalgo‐Martínez ◽  
...  
Keyword(s):  
Tobacco Plants ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Sesquiterpene Cyclase

scholarly journals Putative imbalanced amino acid metabolism in rainbow trout long term fed a plant-based diet as revealed by 1H-NMR metabolomics

2021 ◽  
Vol 10 ◽  
Author(s):  
Catherine Deborde ◽  
Blandine Madji Hounoum ◽  
Annick Moing ◽  
Mickaël Maucourt ◽  
Daniel Jacob ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Proton Nuclear Magnetic Resonance ◽  
Free Form ◽  
Metabolomic Analysis ◽  
Nmr Metabolomics ◽  
H Nmr

Abstract The long-term effect of a plant (P)-based diet was assessed by proton nuclear magnetic resonance (1H-NMR) metabolomics in rainbow trout fed a marine fish meal (FM)–fish oil (FO) diet (M), a P-based diet and a control commercial-like diet (C) starting with the first feeding. Growth performances were not heavily altered by long-term feeding on the P-based diet. An 1H-NMR metabolomic analysis of the feed revealed significantly different soluble chemical compound profiles between the diets. A set of soluble chemical compounds was found to be specific either to the P-based diet or to the M diet. Pterin, a biomarker of plant feedstuffs, was identified both in the P-based diet and in the plasma of fish fed the P-based diet. 1H-NMR metabolomic analysis on fish plasma and liver and muscle tissues at 6 and 48 h post feeding revealed significantly different profiles between the P-based diet and the M diet, while the C diet showed intermediate results. A higher amino acid content was found in the plasma of fish fed the P-based diet compared with the M diet after 48 h, suggesting either a delayed delivery of the amino acids or a lower amino acid utilisation in the P-based diet. This was associated with an accumulation of essential amino acids and the depletion of glutamine in the muscle, together with an accumulation of choline in the liver. Combined with an anticipated absorption of methionine and lysine supplemented in free form, the present results suggest an imbalanced essential amino acid supply for protein metabolism in the muscle and for specific functions of the liver.

NMR metabolic profiling of lipopolysaccharide-induced mice sepsis and the treatment effects of berberine

RSC Advances ◽  
2016 ◽  
Vol 6 (53) ◽  
pp. 47474-47485 ◽  
Author(s):  
Pei Li ◽  
Shanting Liao ◽  
Junsong Wang ◽  
Dingqiao Xu ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Treatment Effects ◽  
Nmr Metabolomics ◽  
H Nmr

1H NMR metabolomics to study lipopolysaccharide-induced mice sepsis and the treatment effects of berberine.

scholarly journals 1H-NMR Metabolomics Analysis of The Intervention Effects of Ruangan Xiaoji Decoction on CCl4 Induced Liver Fibrosis in Rats

2020 ◽  
Author(s):  
Weiliang Zhu ◽  
Guoqing Wu ◽  
Wenrui Zhu ◽  
Tianwen Zhao ◽  
Hong-Jiang Zhang ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Tissue ◽  
Acid Metabolism ◽  
Clinical Chemistry ◽  
Tca Cycle ◽  
Control Group ◽  
Obvious Effect ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Endogenous Metabolites

Abstract Background: Liver fibrosis is a common consequence of chronic liver diseases resulting from multiple etiologies. Early clinical application shows that Ruangan Xiaoji Decoction (RGXJD) has a very obvious effect on the treatment of liver fibrosis. However, the mechanism of RGXJD cures liver fibrosis requires further elucidation.Methods: In this work, the therapeutic effect of RGXJD on CCl4-induced liver fibroses serum and liver tissue metabolite changes in rat was analyzed by 1H-NMR metabolomics. Meanwhile, histopathology examinations and serum clinical chemistry analysis verified the experimental results of metabolomics.Results: RGXJD treatment could reverse the increase in ALT and AST induced by CCl4 and attenuate the pathological changes in liver tissue. In the 1H-NMR metabolomic analysis, PLS-DA score plots demonstrated that the serum and liver tissue metabolic profiles in rats of the RGXJD groups were similar those of the control group, yet remarkably apart from the CCl4 group. The mechanism may be related to the endogenous metabolites including energy metabolism, amino acid metabolism, TCA cycle and purine metabolism in rats. Correlation analysis were then performed to further confirm the metabolites involved in Isoleucine, Tyrosine, UDP-Glucose, Glutathione and Leucine, etc.Conclusions: These findings may provided new insights into the mechanism of the hepatoprotection of RGXJD.

Regulation on BCAAs catabolism pathway plays the key role in cyclophosphamide-induced leucopenia BALB/c mice after the treatment of a typical Traditional Chinese Medicine of Lvjiao Buxue Granules

2020 ◽  
Author(s):  
Jun-sheng Tian ◽  
Hui-liang Zhao ◽  
Qi Wang ◽  
Huan Xiang ◽  
Xiang-ping Xu ◽  
...  
Keyword(s):  
Cancer Chemotherapy ◽  
Blood Cells ◽  
White Blood Cells ◽  
Degradation Pathway ◽  
Integrated Analysis ◽  
Nmr Metabolomics ◽  
Biological Targets ◽  
H Nmr ◽  
Branched Chain ◽  
Endogenous Metabolites

Abstract Background: Cyclophosphamide is a common tumor chemotherapy drug used to treat various cancers, but the resulting immunosuppression leads to leukopenia, which is a serious limiting factor in clinical application. Therefore, the introduction of immunomodulators as adjuvant therapy may help to reduce the hematological side effects of cyclophosphamide. Lvjiao Buxue Granules has been widely used in clinical treatment of gynecological diseases such as anemia and irregular menstruation, and recently, it has been found to increase the role of white blood cells, but its mechanism of action is still unclear. In this research, we applied the 1H-NMR metabolomics approach to characterize metabolites in cyclophosphamide-induced leucopenia mice spleen, so as to fully understand the metabolic processes of leucopenia and improve the leukocyte function of Lvjiao Buxue Granules.Methods: Cyclophosphamide was used to establish the leucopenia mice with cancer chemotherapy and the content of white blood cells, red blood cells, hemoglobin, platelets, and other routine blood indexes were measured. The changes of endogenous metabolites in spleen analyzed by 1H-NMR metabolomics technique were investigated the regulation effect of LBG in mice with leukopenia. Afterward, the chemical components-targets-differential metabolites network of Lvjiao Buxue Granules was constructed by the use of biological targets network, thus leucopenia-relevant metabolism pathways were dissected. Results: The blood routine parameters and organ indexes levels of leucopenia mice with cancer chemotherapy were improved by Lvjiao Buxue Granules. The metabolomic study revealed that 15 endogenous metabolites in mice spleen were considered as potential biomarkers of Lvjiao Buxue Granules for its protective effect. Integrated analysis of metabolomics and biological targets network indicated that Lvjiao Buxue Granules exerted the leukocyte elevation activity by inhibiting the branched-chain amino acids (BCAAs) degradation pathway and increasing the levels of valine, leucine and isoleucine. Conclusion: Lvjiao Buxue Granules exert obvious efficacy on the mice model of leucopenia, which could be improved by regulating the branched-chain amino acid degradation pathway and the levels of valine, leucine and isoleucine.

scholarly journals 1 H NMR metabolomics study of age profiling in children

2009 ◽  
Vol 22 (8) ◽  
pp. 826-833 ◽  
Author(s):  
Haiwei Gu ◽  
Zhengzheng Pan ◽  
Bowei Xi ◽  
Bryan E. Hainline ◽  
Narasimhamurthy Shanaiah ◽  
...  
Keyword(s):  
Nmr Metabolomics ◽  
H Nmr ◽  
Metabolomics Study

Chronic toxicity of crude ricinine in rats assessed by1H NMR metabolomics analysis

RSC Advances ◽  
2015 ◽  
Vol 5 (34) ◽  
pp. 27018-27028 ◽  
Author(s):  
Pingping Guo ◽  
Dandan Wei ◽  
Junsong Wang ◽  
Ge Dong ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Castor Bean ◽  
Chronic Toxicity ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
First Time ◽  
Metabolomics Analysis

A1H-NMR based metabolomics approach combined with OSC-PLS-DA was applied to investigate the chronic toxicity of crude ricinine from castor bean shell in rats for the first time.

scholarly journals Diagnosis of Bovine Respiratory Disease in feedlot cattle using blood 1H NMR metabolomics

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
C. Blakebrough-Hall ◽  
A. Dona ◽  
M. J. D’occhio ◽  
J. McMeniman ◽  
L. A González
Keyword(s):  
Diagnostic Accuracy ◽  
Respiratory Disease ◽  
Bovine Respiratory Disease ◽  
High Accuracy ◽  
Feedlot Cattle ◽  
Disease Classification ◽  
Lung Lesions ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Predictive Algorithms

AbstractCurrent diagnosis methods for Bovine Respiratory Disease (BRD) in feedlots have a low diagnostic accuracy. The current study aimed to search for blood biomarkers of BRD using 1H NMR metabolomics and determine their accuracy in diagnosing BRD. Animals with visual signs of BRD (n = 149) and visually healthy (non-BRD; n = 148) were sampled for blood metabolomics analysis. Lung lesions indicative of BRD were scored at slaughter. Non-targeted 1H NMR metabolomics was used to develop predictive algorithms for disease classification using classification and regression trees. In the absence of a gold standard for BRD diagnosis, six reference diagnosis methods were used to define an animal as BRD or non-BRD. Sensitivity (Se) and specificity (Sp) were used to estimate diagnostic accuracy (Acc). Blood metabolomics demonstrated a high accuracy at diagnosing BRD when using visual signs of BRD (Acc = 0.85), however was less accurate at diagnosing BRD using rectal temperature (Acc = 0.65), lung auscultation score (Acc = 0.61) and lung lesions at slaughter as reference diagnosis methods (Acc = 0.71). Phenylalanine, lactate, hydroxybutyrate, tyrosine, citrate and leucine were identified as metabolites of importance in classifying animals as BRD or non-BRD. The blood metabolome classified BRD and non-BRD animals with high accuracy and shows potential for use as a BRD diagnosis tool.

scholarly journals Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1 H NMR metabolomics

2017 ◽  
Vol 97 (12) ◽  
pp. 4169-4179 ◽  
Author(s):  
Raghunath Pariyani ◽  
Intan Safinar Ismail ◽  
Amalina Ahmad Azam ◽  
Faridah Abas ◽  
Khozirah Shaari
Keyword(s):  
Nmr Metabolomics ◽  
Orthosiphon Stamineus ◽  
H Nmr ◽  
Drying Techniques ◽  
Compositional Changes

Diagnosis of pancreatic cancer via1H NMR metabolomics of human plasma

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 5974-5978 ◽  
Author(s):  
Lenka Michálková ◽  
Štěpán Horník ◽  
Jan Sýkora ◽  
Lucie Habartová ◽  
Vladimír Setnička
Keyword(s):  
Pancreatic Cancer ◽  
Blood Plasma ◽  
Human Plasma ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Potential Biomarkers

The investigation of blood plasma via1H NMR metabolomics revealed a panel of potential biomarkers of pancreatic cancer.

scholarly journals Distinguishing Glycyrrhiza species using NMR-based Metabolomics

2018 ◽  
Vol 13 (1) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Ryuichiro Suzuki ◽  
Fusako Nakano ◽  
Hirokazu Ohno ◽  
Toshiyuki Murakami ◽  
Yoshihito Okada ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Mass Spectra ◽  
Solid Phase ◽  
Food Additives ◽  
Phase Extraction ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Methods Development ◽  
Species Specific ◽  
Natural Medicines

Glycyrrhiza species are widely used as natural medicines and as well as food additives. Quality control during methods development is therefore critically important. In this study, we conducted NMR-based metabolomics analyses to distinguish Glycyrrhiza species ( G. glabra, G. uralensis, and G. inflata). Principle component analysis (PCA) of 1H-NMR spectra of extracts of Glycyrrhiza species enabled categorization of the three species. We conducted solid-phase extraction of methanol extracts of Glycyrrhiza roots prior to NMR measurements. Most studies of Glycyrrhiza species have used mass spectra to distinguish species. By conducting solid-phase extraction prior to NMR analysis, we could distinguish species clearly by PCA score plots. These results indicate that solid-phase extraction enables clear discrimination of Glycyrrhiza species in NMR metabolomics analyses. Furthermore, saponin fractions prepared by solid-phase extraction from water extracts were also discriminated according to the species using NMR metabolomics. Saponins such as glycyrrhizin are important constituents of Glycyrrhiza species. These results thus indicate that there are species-specific differences in root saponins in Glycyrrhiza.

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