Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ

PROTEOMICS ◽  
2019 ◽  
Vol 19 (12) ◽  
pp. 1800387 ◽  
Author(s):  
Lingna Xu ◽  
Lijun Shi ◽  
Lin Liu ◽  
Ruobing Liang ◽  
Qian Li ◽  
...  
1992 ◽  
Vol 67 (03) ◽  
pp. 352-356 ◽  
Author(s):  
Marja Mutanen ◽  
Riitta Freese ◽  
Liisa M Valsta ◽  
Irma Ahola ◽  
Antti Ahlström

SummaryIn this highly controlled trial, 26 normolipidemic men (average age 28 years, range 18 to 60) were fed a baseline diet high in milk fat (MF) (fat 36% of energy, saturates 19%, monounsaturates 11%, polyunsaturates 4%), followed by a diet high in sunflower oil (SO) (fat 38% of energy, saturates 13%, monounsaturates 10%, polyunsaturates 13%) and another diet high in low erucic-acid rapeseed oil (RO) (fat 38% of energy, saturates 12%, monounsaturates 16%, polyunsaturates 8%). All diets were mixed natural diets with the same cholesterol contents. The baseline milk fat diet was given for 14 days and the oil diets for 24 days in a blind cross-over design. The platelet in vitro aggregation (slope %/min) induced by 1, 2 and 3 pM ADP and collagen (25 pg/ml PRP) was highly significantly (p <0.001) increased after both oil diets when compared with the results from the milk fat diet. The aggregation pattern determined by threshold collagen concentration confirmed increased collagen sensitivity of the platelets after the rapeseed oil diet (p <0.001). The enhancement of platelet aggregation was associated with increased in vitro platelet thromboxane production after the oil diets vs. the milk fat diet (p <0.05 after the sunflower oil diet and p <0.001 after the rapeseed oil diet).


Author(s):  
Prof. Asoc. Dr. Shurki MAXHUNI ◽  
Prof.Asiss.Dr.Nerimane BAJRAKTARI

The dairy industry seems to have convinced the food industry that whey is a miracle product. The list of supposed benefits it gives to food is as long as your arm. Some of the benefits may be real. Whey is the liquid remaining after milk has been curdled and strained. It is a by-product of the manufacture of cheese or casein and has several commercial uses. To produce cheese, rennet or an edible acid is added to heated milk. This makes the milk coagulate or curdle, separating the milk solids (curds) from the liquid whey. Sweet whey is the byproduct of rennet-coagulated cheese and acid whey (also called sour whey) is the byproduct of acid-coagulated cheese. Sweet whey has a pH greater than or equal to 5.6, acid whey has a pH less than or equal to 5.1. Whey is also a great way to add sweetness to a product without having to list sugar as an ingredient as whey contains up to 75% lactose. And it sounds healthy. This study is done to research the examinations for the production of mozzarella cheese from Cow’s milk, after research and analyses of a physical-chemical peculiar feature of whey from coagulum. We have followed the processes from the drying of whey from the coagulum analyzer's physical-chemical peculiar feature. We carried out three experiments. For every experiment, we took three patterns and analyzed the physical-chemical. The calculation was appraised statistically. This paper deals with the research of% of whey fat during the process of milk production from standardized to non-standardized milk. Where% of whey fat should be an economic indicator for standardizing milk for dairy production.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ryszard Mordak ◽  
Zbigniew Dobrzański ◽  
Robert Kupczyński

AbstractTesting blood and milk parameters as well as analysing the relationships among these markers is very useful for monitoring the internal homeostasis and health in high-yielding dairy cows during various production periods. The aim of the study was to assess the correlations (relationships) among macro-minerals, such as calcium (Ca), inorganic phosphorus (P), magnesium (Mg), other selected bone profile markers, such as total protein (TP), albumin, activity of alkaline phosphatase (ALP) measured in serum and selected milk components such as number of somatic cells (SCC), colony-forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), dry matter (DM), non-fat dry matter (FDM) and milk production in late-lactation cows. Both blood and milk samples were collected from 11 clinically healthy milking cows during the late-lactation period. The cows were examined once a day for 3 consecutive days resulting in 33 sets of blood and milk samples for laboratory and statistical analysis. Significant correlations were observed between: Mg and MP, Mg and FDM, ALP and SCC, TP and SCC, TP and MP, TP and FDM, albumin and MP, albumin and FDM, P and Mg, Mg and albumin, and between TP and albumin. When monitoring macro-mineral homeostasis and mammary gland health, especially in intensively fed high-yielding dairy cows correlations between these markers should be considered. The revealed correlations can allow for deeper comparative laboratory diagnostics of homeostasis and can be especially useful for laboratory monitoring of the potential risk of subclinical macro-mineral deficiency in high-yielding dairy cows.


2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 418-418
Author(s):  
F. Rosa ◽  
J. S. Osorio ◽  
J. Lohakare ◽  
M. Moridi ◽  
A. Ferrari ◽  
...  

Author(s):  
K.ZH. ZHUMANOV ◽  
◽  
T.N. KARYMSAKOV ◽  
M.A. KINEEV ◽  
M.V. TAMAROVSKIY ◽  
...  

According to the current “Instruction” used in dairy cattle selection and breeding in the Republic of Kazakhstan, bulls-producers of dairy breeds are assessed according to the their offspring quality based on the principle of “peer daughter”. This means that the phenotypic indicators of the daughters of the tested bulls are compared with the corresponding indicators of their peers. In European countries with developed dairy cattle breeding, as well as in Canada, the USA, etc., to ensure a reliable forecast of the genetic value of individuals (primarily, bulls-producers), use is made of the best linear unbiased forecast method (BLUP method). This method implies that the breeding value of producers is determined by the deviation values of the development of traits of the examined animal from its average values in the population. Especially urgent area is the research aimed at improving breeding programs, including assessing the breeding value of bulls-producers of dairy breeds using BLUP methods based on the productive qualities of the mass of dairy cattle in the Republic of Kazakhstan. The research material included the data on the phenotypic indicators of the milk productivity of first-calf cows (the amount of milk yield, the content of fat and protein in milk, the yield of milk fat and protein) of the Holstein black-motley dairy cattle breed, obtained from the information and analytical database of the Republic of Kazakhstan for 2016–2017. It was found that when evaluating according to the official “Instruction”, 16 sires out of 256 bulls (6.2%) got the stud category in 2016, 14 sires (9.2%) out of 152 bulls in 2017, and – 30 sires of 249 bulls (12.0%) over the cumulative period. The results of the conducted research prove that the use of the classic “Instructions” in dairy cattle breeding has lower efficiency (by 42.8–90.0%) as compared with the assessment of the breeding value of bulls based on the BLUP method.The selection of sire bulls into breeding groups based on the “peer daughter” methodology is not reliable enough and rather ineffective. Comparing the results of assessing the breeding qualities of sire bulls, obtianed using two methods in all compared periods (2016, 2017, 2016–2017), the authors established a clear superiority of the BLUP method over the current Instruction used in the Republic of Kazakhstan.


2016 ◽  
Vol 52 ◽  
pp. 194-202
Author(s):  
S. L. Voitenko ◽  
L. V. Vishnevsky

The article shows the state of Ukrainian Whiteheaded cattle, which includes distribution of cattle, the number of animals belonging to respective bloodlines, evaluation of young animals with live weight in the process of growing and milk production of cows during the first lactation. It reflects the historic development of the breed when it was colonism whiteheaded cattle, which turned into the original breed, undergone a significant expansion in livestock and increase of productivity, decreased in the number, was as basis for creation of Ukrainian Black-and-White dairy breed and now bred only in one breeding farm. Visual estimation of animal exterior showed good development of cows and calves and their belonging to the dairy type. In the vast majority the cows of the herd have a black suit, a white head with " glasses" around the eyes, white belly, udder, lower legs and brush of the tail. The youngsters aren’t consolidated by the exterior, and among them there are animals which are not typical for Ukrainian Whiteheaded breed. The young animals have some lag in live weight behind the breed standard [12] to 7 months’ age with exceeding of this trait in certain periods quite significantly in the future. It was established that selection of heifers on live weight will be effective at the early age (1-5 months), given the coefficient of variation of live weight – 22,63-30,21% and will not have a significant influence in the future. Milk yields of first-calf heifers vary considerably depending on the origin. The milk yield of first-calf heifers in the herd was 4238,5 kg on average, the heifers belonging to Mart 171 and Ozon 417 bloodlines had the best milk performance – 4483,1 and 4254,9 kg accordingly. The most aligned milk yield during the first lactation was in the cows belonging to Ozon 417 bloodline, the limits of the trait are 4128,5-4327,4 kg with the average value by the line 4254,9 kg. In contrast, the first-calf heifers of Ryezvyi 33 bloodline with average milk yield 4048,9 kg had limits of the trait 2199,3-4736,1 kg. Even greater range in cows’ milk yield during the first lactation R= 4939 kg (limits 1687 – 6626 kg) is characterized for the herd in general, it shows, on the one hand, the possibility of qualitative improvement of cows’ productivity due to selection on the investigated trait and lack of selection in the herd on the other hand. It was established that daughters of bull Chardash belonging to Ryezvyi 33 bloodline produced 4736,1 kg of milk for 305 days of the first lactation with fat content 3,6%, whereas Zlak’s descendants of the same line were characterized by the lowest milk yield for the first completed lactation – 2199,3 kg with fat content 3,7% and the average value by the line – 4048,9 kg of milk, fat content 3,6%. Similar variability of first-calf heifers’ milk yields, depending on the origin, is typical for other bloodlines of Ukrainian Whiteheaded breed. To increase milk productivity of Ukrainian Whiteheaded cows is recommended to repeat successful combinations of parental forms, and to preserve the breed – to carry out an objective assessment of animals by a range of traits, given the efficiency of selection of heifers on live weight at early age.


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