Urine Proteome Changes in a TNBS‐Induced Colitis Rat Model

Background: Statin-associated muscle symptoms (SAMS) are the main side effects of statins. Currently, there are no effective biomarkers for accurate clinical diagnosis. Urine is not subject to homeostatic control and therefore accumulates early changes, making it an ideal biomarker source. We therefore examined urine proteome changes associated with SAMS in an animal model. Methods: Here, we established a SAMS rat model by intragastric intubation with simvastatin (80 mg/kg). Biochemical analyses and hematoxylin and eosin (H&E) staining were used to evaluate the degree of muscle injury. The urine proteome on days 3, 6, 9 and 14 was profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) with the data-independent acquisition (DIA) method. Results: Differential proteins on day 14 of SAMS were mainly associated with glycolysis/gluconeogenesis, pyruvate metabolism, metabolism of reactive oxygen species and apoptosis, all of which were reported to be associated with the pathological mechanism of SAMS. Among the 14 differentially expressed proteins on day 3, FIBG, OSTP and CRP were associated with muscle damage, while EHD1, CUBN and FINC were associated with the pathogenic mechanisms of SAMS. MYG and PRVA increased dramatically compared with CK elevation in serum on day 14 of SAMS. Conclusions: Our preliminary results indicated that the urine proteome can reflect early changes in the SAMS rat model, providing the potential for monitoring drug side effects in future clinical research. Keywords: Urine proteome, statin-associated muscle symptoms, animal model, biomarkers

Download Full-text

Early urine proteome changes in the Walker-256 tail-vein injection rat model

Scientific Reports ◽

10.1038/s41598-019-50301-1 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Jing Wei ◽

Na Ni ◽

Wenshu Meng ◽

Youhe Gao

Keyword(s):

B Cell ◽

Rat Model ◽

Lung Tumor ◽

Whole Body ◽

Positive Regulation ◽

Urine Proteome ◽

Tail Vein ◽

Tail Vein Injection ◽

Differential Proteins ◽

Walker 256

Abstract Detection of cancer at its early stage is important for treatment. Urine, which is not regulated by homeostatic mechanisms, reflects early systemic changes throughout the whole body and can be used for the early detection of cancer. In this study, the Walker-256 tail-vein injection rat model was established to find whether the urine proteome could reflect early changes if tumor grown in lung. Urine samples from the control group (n = 7) and Walker-256 tail-vein injection group (n = 7) on days 2, 4, 6 and 9 were analyzed by label-free proteomic quantitative methods. On day 2, when lung tumor nodules did not appear, 62 differential proteins were identified. They were associated with epithelial cell differentiation, regulation of immune system processes and the classical complement activation pathway. On day 4, when lung tumor nodules appeared, 72 differential proteins were identified. They were associated with the innate immune response and positive regulation of phagocytosis. On day 6, when body weight began to decrease, 117 differential proteins were identified. On day 9, the identified 125 differential proteins were associated with the B cell receptor signaling pathway and the positive regulation of B cell activation. Our results indicate that (1) the urine proteome changed even on the second day after tail-vein injection of Walker-256 cells and that (2) compared to previous studies, the urine proteomes were different when the same cancer cells were grown in different organs.

Download Full-text

Comparison of urine proteome among rat models by intraperitoneal injection with single bacteria and co-injection with two bacteria

PLoS ONE ◽

10.1371/journal.pone.0261488 ◽

2021 ◽

Vol 16 (12) ◽

pp. e0261488

Author(s):

Wenshu Meng ◽

Chenyang Zhao ◽

Youhe Gao

Keyword(s):

Mass Spectrometry ◽

Intraperitoneal Injection ◽

Single Injection ◽

Tandem Mass ◽

Human Pathogens ◽

Rat Models ◽

Urine Proteome ◽

Differential Proteins ◽

Proteome Changes ◽

S Model

Purpose To explore and compare urine proteome changes among rat models by intraperitoneal injection with single bacteria and co-injection with two bacteria. Method Escherichia coli and Staphylococcus aureus are two common human pathogens. Three rat models were established: (i) the intraperitoneal co-injection of E. coli and S. aureus model (ES model), (ii) intraperitoneal injection of E. coli model (E model), and (iii) intraperitoneal injection of S. aureus model (S model). Urinary proteomes on days 0, 1 and 2 of the three models were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Results A total of 111, 34 and 94 differential proteins were identified in the ES model, E model and S model, respectively. Among them, some differential proteins were reported to be associated with bacterial infection. Approximately 47% differential proteins in the E model overlapped with ES model, and 37% differential proteins in the S model overlapped with ES model. Compared with the E model and S model, a total of 71 unique differential proteins were identified in the ES model. Conclusion Our results indicated that (1) the urine proteome could distinguish different bacterial intraperitoneal injections models and (2) the effects of co-injection with two bacteria on the urine proteome were not simple superposition of single injection.

Download Full-text

Dynamic changes of urine proteome in a Walker 256 tumor-bearing rat model

Cancer Medicine ◽

10.1002/cam4.1225 ◽

2017 ◽

Vol 6 (11) ◽

pp. 2713-2722 ◽

Cited By ~ 29

Author(s):

Jianqiang Wu ◽

Zhengguang Guo ◽

Youhe Gao

Keyword(s):

Rat Model ◽

Dynamic Changes ◽

Urine Proteome ◽

Tumor Bearing ◽

Walker 256 ◽

Walker 256 Tumor

Download Full-text

Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2021.114064 ◽

2021 ◽

pp. 114064

Author(s):

Yuhang Huan ◽

Jing Wei ◽

Tong Su ◽

Youhe Gao

Keyword(s):

Major Depressive Disorder ◽

Mouse Model ◽

Depressive Disorder ◽

Mild Stress ◽

Chronic Unpredictable Mild Stress ◽

Major Depressive ◽

Urine Proteome ◽

Proteome Changes

Download Full-text

Urine proteome changes associated with autonomic regulation of heart rate in cosmonauts

BMC Systems Biology ◽

10.1186/s12918-019-0688-9 ◽

2019 ◽

Vol 13 (S1) ◽

Cited By ~ 2

Author(s):

Lyudmila H. Pastushkova ◽

Vasily B. Rusanov ◽

Anna G. Goncharova ◽

Alexander G. Brzhozovskiy ◽

Alexey S. Kononikhin ◽

...

Keyword(s):

Heart Rate ◽

Autonomic Regulation ◽

Urine Proteome ◽

Proteome Changes

Download Full-text

Distinct brain regional proteome changes in the rTg‐DI rat model of cerebral amyloid angiopathy

Journal of Neurochemistry ◽

10.1111/jnc.15463 ◽

2021 ◽

Author(s):

Joseph M. Schrader ◽

Feng Xu ◽

William E. Van Nostrand

Keyword(s):

Cerebral Amyloid Angiopathy ◽

Rat Model ◽

Amyloid Angiopathy ◽

Cerebral Amyloid ◽

Proteome Changes

Download Full-text

Urine proteome changes in an α-synuclein transgenic mouse model of Parkinson’s disease

10.1101/2020.04.05.026104 ◽

2020 ◽

Author(s):

Lujun Li ◽

Xuanzhen Pan ◽

Ting Wang ◽

Yuanrui Hua ◽

Youhe Gao

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Transgenic Mice ◽

Urine Proteome ◽

Data Independent Acquisition ◽

Chromatography Tandem Mass Spectrometry ◽

Differential Proteins ◽

Liquid Chromatography Tandem Mass ◽

Proteome Changes ◽

Old Mice

AbstractUrine accommodates more changes than other fluids, and it is a good source in the search for early sensitive biomarkers. The present study collected urine samples from 2-, 4-, 6-, 8- and 10-month-old α-synuclein transgenic mice. Based on data-independent acquisition (DIA) technology, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for quantitative analysis. Seventeen human homologous differential proteins were screened and compared with those in the urine of 2-month-old mice, and 9 proteins were related to Parkinson’s disease (PD). Formin-2, Splicing factor 3A subunit 1, and Isopentenyl-diphosphate Delta-isomerase 1 changed continuously in months 6, 8 and 10. These experiments and analyses demonstrated that the urine proteome reflected the development of α-synuclein transgenic mice and provided clues for the early clinical diagnosis of PD.

Download Full-text