scholarly journals A LAMP (Loop‐mediated isothermal amplification) test for rapid identification of Khapra beetle ( Trogoderma granarium )

2021 ◽  
Author(s):  
Lea Rako ◽  
Arati Agarwal ◽  
Linda Semeraro ◽  
Adam Broadley ◽  
Brendan C Rodoni ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Trogoderma Granarium ◽  
Khapra Beetle

scholarly journals Rapid identification of transgenic cotton (Gossypium hirsutum L.) plants by loop-mediated isothermal amplification

2011 ◽  
Vol 47 (No. 4) ◽  
pp. 140-148 ◽  
Author(s):  
N. Rostamkhani ◽  
A. Haghnazari ◽  
M. Tohidfar ◽  
A. Moradi
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Lamp Assay ◽  
Transgenic Cotton ◽  
Rapid Identification ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Lamp Method ◽  
Loop Mediated Isothermal Amplification ◽  
Leaf Tissues

In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was extracted from fresh leaf tissues of T<sub>2</sub> transgenic cotton containing chitinase (chi) and cry1A(b) genes. Detection of genes of interest was performed by polymerase chain reaction (PCR), LAMP and real-time PCR methods. In LAMP assay the amplification was performed after 30 min at 65&deg;C when loop primers were involved in the reaction. The involvement of loop primers decreased the time needed for amplification. By testing serial tenfold dilutions (10<sup>&ndash;1</sup> to 10<sup>&ndash;8</sup>) of the genes of interest, the detection sensitivity of LAMP was found to be 100-fold higher than that of PCR. The rapid DNA extraction method and LAMP assay can be performed within 30 min and the derived LAMP products can be directly observed as visually detectable based on turbidity in the reaction tube. The accuracy of LAMP method in the screening of transgenes was confirmed by PCR and real-time PCR. The developed method was efficient, rapid and sensitive in the screening of cotton transgenic plants. This method can be applied to any other crops.

scholarly journals Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Masashi Kitamura ◽  
Akira Kazato ◽  
Tadashi Yamamuro ◽  
Hirokazu Ando ◽  
Yohei Sasaki ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Rapid identification of RNA‐interference‐based resistance to Bean golden mosaic virus in transgenic common beans via loop‐mediated isothermal amplification

Crop Science ◽  
2020 ◽  
Vol 60 (6) ◽  
pp. 3004-3012
Author(s):  
Nara Cristina Teixeira ◽  
Adriane Wendland ◽  
Maythsulene Inácio de Souza Oliveira ◽  
Livia Teixeira Duarte Brandão ◽  
Thiago Lívio Pessoa Oliveira Souza ◽  
...  
Keyword(s):  
Rna Interference ◽  
Mosaic Virus ◽  
Rapid Identification ◽  
Isothermal Amplification ◽  
Common Beans ◽  
Bean Golden Mosaic Virus ◽  
Loop Mediated Isothermal Amplification

scholarly journals Rapid Identification of the Mediterranean Fruit Fly (Diptera: Tephritidae) by Loop-Mediated Isothermal Amplification

2009 ◽  
Vol 102 (3) ◽  
pp. 1239-1246 ◽  
Author(s):  
Chin-Gi Huang ◽  
Ju-Chun Hsu ◽  
David S. Haymer ◽  
Guo-Cih Lin ◽  
Wen-Jer Wu
Keyword(s):  
Fruit Fly ◽  
Rapid Identification ◽  
Mediterranean Fruit Fly ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
The Mediterranean

Loop-Mediated Isothermal Amplification Assays for the Rapid Identification of Duck-Derived Ingredients in Adulterated Meat

2017 ◽  
Vol 10 (7) ◽  
pp. 2325-2331 ◽  
Author(s):  
Ya Shi ◽  
Yan Feng ◽  
Changping Xu ◽  
Zhouheng Xu ◽  
Dongqing Cheng ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification

2009 ◽  
Vol 156 (1-2) ◽  
pp. 32-36 ◽  
Author(s):  
Namal Perera ◽  
Hiroka Aonuma ◽  
Aya Yoshimura ◽  
Tokiyasu Teramoto ◽  
Hiroshi Iseki ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Rapid Identification ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification
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