Vacuolar (H + )‐ ATPase subunit c is essential for the survival and systemic RNA interference response in Locusta migratoria

2022 ◽  
Author(s):  
Xuekai Shi ◽  
Xiaojian Liu ◽  
Anastasia M.W. Cooper ◽  
Kristopher Silver ◽  
Hans Merzendorfer ◽  
...  
Keyword(s):  
Rna Interference ◽  
Locusta Migratoria ◽  
Atpase Subunit ◽  
Subunit C ◽  
Systemic Rna ◽  
Interference Response

Instar-dependent systemic RNA interference response in Leptinotarsa decemlineata larvae

2015 ◽  
Vol 123 ◽  
pp. 64-73 ◽  
Author(s):  
Wen-Chao Guo ◽  
Kai-Yun Fu ◽  
Shuai Yang ◽  
Xiao-Xu Li ◽  
Guo-Qing Li
Keyword(s):  
Rna Interference ◽  
Leptinotarsa Decemlineata ◽  
Systemic Rna ◽  
Interference Response

scholarly journals Tissue-dependence and sensitivity of the systemic RNA interference response in the desert locust, Schistocerca gregaria

2012 ◽  
Vol 42 (12) ◽  
pp. 911-917 ◽  
Author(s):  
Niels Wynant ◽  
Heleen Verlinden ◽  
Bert Breugelmans ◽  
Gert Simonet ◽  
Jozef Vanden Broeck
Keyword(s):  
Rna Interference ◽  
Schistocerca Gregaria ◽  
Desert Locust ◽  
Systemic Rna ◽  
Interference Response

An intact RNA interference pathway is required for expression of the mutant wing phenotype of vg21-3, a P-element-induced allele of the vestigial gene in Drosophila

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 312-326 ◽  
Author(s):  
Ross B. Hodgetts ◽  
Sandra L. O’Keefe ◽  
Kyle J. Anderson
Keyword(s):  
Rna Interference ◽  
Dna Sequencing ◽  
Mutant Phenotype ◽  
P Element ◽  
Type I ◽  
P Elements ◽  
Gene Encode ◽  
Interference Response ◽  
Partial Reversion

We have determined that two P elements, P[21-3] and P[21r36], residing in the 5′-UTR of the vestigial wing gene, encode functional repressors in eye tissue. However, neither element fits a previous categorization of repressor-making elements as Type I or II. Both elements encode polypeptides that are shorter than the canonical elements they most closely resemble. DNA sequencing reveals that P[21r36] encodes an intact THAP domain that is missing in the P[21] element, which does not encode a functional repressor. Recovery of P[21-3] at sites other than vestigial (where it causes the wing mutant, vg21-3) reveals that the element can make repressor in wing tissue of sufficient activity to repress the mutant phenotype of vg21-3. Why the P[21-3] element fails to produce repressor when located at vestigial may be explained by our observation that three different mutants in the RNA interference pathway cause a partial reversion of vg21-3. We speculate that the vg and P-initiated transcripts that arise at the vg locus in the vg21-3 mutant trigger an RNA interference response that results in the mutual degradation of both transcripts.

scholarly journals New insight into the RNA interference response against cathepsin-L gene in the pea aphid, Acyrthosiphon pisum: Molting or gut phenotypes specifically induced by injection or feeding treatments

2014 ◽  
Vol 51 ◽  
pp. 20-32 ◽  
Author(s):  
Panagiotis Sapountzis ◽  
Gabrielle Duport ◽  
Séverine Balmand ◽  
Karen Gaget ◽  
Stéphanie Jaubert-Possamai ◽  
...  
Keyword(s):  
Rna Interference ◽  
Acyrthosiphon Pisum ◽  
Cathepsin L ◽  
Pea Aphid ◽  
Interference Response ◽  
Insight Into

Lethal RNA interference response in the pepper weevil

2019 ◽  
Vol 143 (7) ◽  
pp. 699-705 ◽  
Author(s):  
Ke Wu ◽  
Caitlin E. Taylor ◽  
Daniele H. Pinheiro ◽  
Lucile H. Skelley ◽  
Heather J. McAuslane ◽  
...  
Keyword(s):  
Rna Interference ◽  
Pepper Weevil ◽  
Interference Response

scholarly journals Novel Role of ATPase Subunit C Targeting Peptides Beyond Mitochondrial Protein Import

2010 ◽  
Vol 21 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Cristofol Vives-Bauza ◽  
Jordi Magrané ◽  
Antoni L. Andreu ◽  
Giovanni Manfredi
Keyword(s):  
Respiratory Chain ◽  
Protein Import ◽  
Mitochondrial Protein ◽  
Atp Synthesis ◽  
Mitochondrial Respiratory Chain ◽  
Genetic Redundancy ◽  
Mitochondrial Protein Import ◽  
Atpase Subunit ◽  
Subunit C ◽  
And Function

In mammals, subunit c of the F1F0-ATP synthase has three isoforms (P1, P2, and P3). These isoforms differ by their cleavable mitochondrial targeting peptides, whereas the mature peptides are identical. To investigate this apparent genetic redundancy, we knocked down each of the three subunit c isoform by RNA interference in HeLa cells. Silencing any of the subunit c isoforms individually resulted in an ATP synthesis defect, indicating that these isoforms are not functionally redundant. We found that subunit c knockdown impaired the structure and function of the mitochondrial respiratory chain. In particular, P2 silencing caused defective cytochrome oxidase assembly and function. Because the expression of exogenous P1 or P2 was able to rescue the respective silencing phenotypes, but the two isoforms were unable to cross-complement, we hypothesized that their functional specificity resided in their targeting peptides. In fact, the expression of P1 and P2 targeting peptides fused to GFP variants rescued the ATP synthesis and respiratory chain defects in the silenced cells. Our results demonstrate that the subunit c isoforms are nonredundant, because they differ functionally by their targeting peptides, which, in addition to mediating mitochondrial protein import, play a yet undiscovered role in respiratory chain maintenance.

scholarly journals Inducible Systemic RNA Silencing in Caenorhabditis elegans

2003 ◽  
Vol 14 (7) ◽  
pp. 2972-2983 ◽  
Author(s):  
Lisa Timmons ◽  
Hiroaki Tabara ◽  
Craig C. Mello ◽  
Andrew Z. Fire
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Rna Silencing ◽  
Molecular Mechanisms ◽  
Normal Animal ◽  
Cell Types ◽  
Animal Cells ◽  
Tissue Specific ◽  
C Elegans ◽  
Systemic Rna

Introduction of double-stranded RNA (dsRNA) can elicit a gene-specific RNA interference response in a variety of organisms and cell types. In many cases, this response has a systemic character in that silencing of gene expression is observed in cells distal from the site of dsRNA delivery. The molecular mechanisms underlying the mobile nature of RNA silencing are unknown. For example, although cellular entry of dsRNA is possible, cellular exit of dsRNA from normal animal cells has not been directly observed. We provide evidence that transgenic strains of Caenorhabditis elegans transcribing dsRNA from a tissue-specific promoter do not exhibit comprehensive systemic RNA interference phenotypes. In these same animals, modifications of environmental conditions can result in more robust systemic RNA silencing. Additionally, we find that genetic mutations can influence the systemic character of RNA silencing in C. elegans and can separate mechanisms underlying systemic RNA silencing into tissue-specific components. These data suggest that trafficking of RNA silencing signals in C. elegans is regulated by specific physiological and genetic factors.

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