Stem Cell Factor and Interleukin-3 Induce Stepwise Generation of Erythroid Precursor Cells from a Basic Fibroblast Growth Factor-Dependent Hematopoietic Stem Cell Line, A-6

Abstract Background: Gastrointestinal tract function and it's integrity are controlled by a number of peptides whose secretion is influenced by severe inflammation. In stomach the main regulatory peptide is ghrelin. For upper small intestine cholecystokinin and lower small intestine glucagon-like peptide- 1 are secreted, while fibroblast growth factor-21 is secreted by several organs, including the liver, pancreas, and adipose tissue [12]. Hematopoietic stem cell transplantation causes serious mucosal damage, which can reflect on this peptides. Methods: The aim of the study was to determine fasting plasma concentrations of ghrelin, cholecystokinin, glucagon- like peptide-1, and fibroblast growth factor -21, and their gene expressions, before and 6 months after hematopoietic stem cell transplantation. 27 children were studied, c ontrol group included 26 healthy children. Results: Acute graft versus host disease was diagnosed in 11 patients (41%, n=27). Median pre-transplantation concentrations of gastrointestinal peptides , as well as their gene expressions, were significantly lower in studied group compared with the control group. Only median of fibroblast growth factor-21 concentration was near-significantly higher before stem cell transplantation than in the control group. The post–hematopoietic transplant results revealed significantly higher concentrations of the studied peptides (except fibroblast growth factor-21) and respective gene expressions as compare to pre transplant results . M edian glucagone like peptide-1 concentrations were significantly decreased in patients with features of acute graft versus host disease . Moreover, negative correlation between glucagone like peptide-1 concentrations and acute graft versus host disease severity was found. Conclusions: Increased concentrations and gene expressions of gastrointestinal tract regulation peptides can be caused by stimulation of regeneration in the severe injured organ . Measurement of these parameters may be a useful method of assessment of severity of gastrointestinal tract complications of hematopoietic stem cell transplantation.

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Effects of 6-bromoindirubin-3′-oxime on the maintenance of pluripotency of porcine embryonic germ cells in combination with stem cell factor, leukemia inhibitory factor and fibroblast growth factor

Reproduction ◽

10.1530/rep-09-0539 ◽

2010 ◽

Vol 139 (6) ◽

pp. 1039-1046 ◽

Cited By ~ 6

Author(s):

Jiang Wen ◽

Juan Liu ◽

Guangqi Song ◽

Limei Liu ◽

Bo Tang ◽

...

Keyword(s):

Stem Cell ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Germ Cells ◽

Leukemia Inhibitory Factor ◽

Stem Cell Factor ◽

Glycogen Synthase ◽

Embryoid Bodies ◽

Inhibitory Factor ◽

Fibroblast Growth

6-Bromoindirubin-3′-oxime (BIO), which is one of the glycogen synthase kinase 3 inhibitors and a key regulator of numerous signaling pathways, was reported to be capable of maintaining the pluripotency of human and mouse embryonic stem cells. Presently, it is unknown whether BIO can influence the derivation of porcine embryonic germ (EG) cells. In this study, porcine primordial germ cells (PGCs) were isolated from gonads of 24- and 28-day embryos, and were then treated with BIO either individually or in combination with other cytokines (stem cell factor (SCF), leukemia inhibitory factor (LIF), and fibroblast growth factor (FGF); abbreviated as ‘3F’), and the effects of the treatment on the proliferation ability of porcine PGCs at early stage were examined using 5-bromo-2-deoxyuridine (Brdu) immunostaining assay. After continuous culture, the effects on the efficiency of porcine undifferentiated EG cells in the third passage and differentiated EG cells from embryoid bodies were examined as well. The results obtained through the observation of the Brdu-labeled PGCs indicated that BIO in combination with 3F resulted in a significant increase in the mitosis index, and also indicated that the BIO in combination with 3F had a higher efficiency in promoting the formation of porcine EG colony derived from porcine day 24 PGCs than BIO used either individually or in combination with LIF. In addition, BIO in combination with 3F exhibited the apparent anti-differentiation activity by reversing the differentiated EG cells to the undifferentiated status. Our results demonstrate that BIO in combination with SCF, LIF, and FGF could significantly contribute to the establishment of a porcine EG cell colony and maintain the undifferentiated status.

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Stem cell factor and basic fibroblast growth factor are synergistic in augmenting committed myeloid progenitor cell growth

Blood ◽

10.1182/blood.v83.4.907.907 ◽

1994 ◽

Vol 83 (4) ◽

pp. 907-910 ◽

Cited By ~ 47

Author(s):

JL Gabrilove ◽

K White ◽

Z Rahman ◽

EL Wilson

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Growth Factor ◽

Cell Growth ◽

Fibroblast Growth Factor ◽

Stromal Cells ◽

Stem Cell Factor ◽

Progenitor Cell ◽

Fibroblast Growth ◽

Gm Csf

Abstract Stem cell factor (SCF) and basic fibroblast growth factor (bFGF) are hematopoietic cytokines produced by bone marrow stromal cells. It is known that, although SCF and bFGF have limited clonogenic activity on their own, they can augment colony-stimulating factor (CSF)-mediated progenitor cell growth. Because these factors are both sequestered by stromal cells, we examined their interaction on progenitor cell growth in conjunction with granulocyte-macrophage-CSF (GM-CSF). In this study, we show that clonogenic growth derived from low-density bone marrow cells stimulated by GM-CSF is significantly augmented (P < .001) in the presence of maximal (100 ng/mL) concentrations of SCF in combination with 100 ng/mL of bFGF. When CD34+ cells are used, the synergistic effect of bFGF and SCF for GM-CSF-mediated progenitor cell growth is further increased, resulting in as much as a sevenfold increase in detectable colony-forming units granulocyte-macrophage (P < .001). These data suggest that the synergistic activity of bFGF and SCF is mediated directly on hematopoietic precursors. These observations suggest that bFGF and SCF, concentrated locally on stromal cell surfaces, might interact in concert with other hematopoietic cytokines to regulate stem cell proliferation and differentiation in hematopoietic niches in the bone marrow.

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Retrovirus-mediated transfer of the human O6-Methylguanine-DNA methyltransferase gene into a murine hematopoietic stem cell line and resistance to the toxic effects of certain alkylating agents

Biochemical Pharmacology ◽

10.1016/0006-2952(96)00077-9 ◽

1996 ◽

Vol 51 (9) ◽

pp. 1221-1228 ◽

Cited By ~ 13

Author(s):

Wang Gang ◽

Clifford Weiss ◽

Peihua Sheng ◽

Edward Bresnick

Keyword(s):

Stem Cell ◽

Cell Line ◽

Hematopoietic Stem Cell ◽

Dna Methyltransferase ◽

Alkylating Agents ◽

Stem Cell Line ◽

Hematopoietic Stem ◽

Methylguanine Dna Methyltransferase ◽

Methyltransferase Gene ◽

Murine Hematopoietic Stem Cell

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Fetal hematogenous routing of a donor hematopoietic stem cell line in a healthy syngeneic model of transamniotic stem cell therapy

Journal of Pediatric Surgery ◽

10.1016/j.jpedsurg.2021.02.035 ◽

2021 ◽

Author(s):

Stefanie P. Lazow ◽

Ina Kycia ◽

Daniel F. Labuz ◽

David Zurakowski ◽

Dario O. Fauza

Keyword(s):

Stem Cell ◽

Cell Therapy ◽

Cell Line ◽

Hematopoietic Stem Cell ◽

Stem Cell Therapy ◽

Stem Cell Line ◽

Hematopoietic Stem ◽

Syngeneic Model

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Peptides regulating gastrointestinal function in children before and after hematopoietic stem cell transplantation

10.21203/rs.2.16711/v1 ◽

2019 ◽

Author(s):

Magdalena Rej ◽

Szymon Skoczeń ◽

Danuta Pietrys ◽

Kinga Kwiecińska ◽

Przemysław J. Tomasik ◽

...

Keyword(s):

Stem Cell ◽

Growth Factor ◽

Hematopoietic Stem Cell Transplantation ◽

Stem Cell Transplantation ◽

Fibroblast Growth Factor ◽

Cell Transplantation ◽

Fibroblast Growth Factor 21 ◽

Fibroblast Growth ◽

Gene Expressions ◽

Hematopoietic Stem

Abstract Gastrointestinal tract function and it's integrity are controlled by a number of peptides whose secretion is influenced by severe inflammation. In stomach the main regulatory peptide is ghrelin. For upper small intestine cholecystokinin and glucagon-like peptide- 1 are secreted, while fibroblast growth factor-21 is secreted by several organs, including the liver, pancreas, and adipose tissue [12]. Hematopoietic stem cell transplantation causes serious mucosal damage, which can reflect on this peptides. The aim of the study was to determine fasting plasma concentrations of ghrelin, cholecystokinin, glucagone- like peptide-1, and fibroblast growth factor -21, and their gene expressions, before and 6 months after hematopoietic stem cell transplantation. 27 children were studied. C ontrol group included 26 healthy children. Acute graft versus host disease was diagnosed in 11 patients (41%, n=27). Median pre-transplantation concentrations of ghrelin, cholecystokinin , and glucagone like peptide-1, as well as their gene expressions, were significantly lower compared with the control group. In contrast, median fibroblast growth factor-21 concentrations were near-significantly higher before stem cell transplantation than in the control group. A comparison of pre- and post-transplantation groups revealed significantly higher concentrations of the studied peptides (except fibroblast growth factor-21) and respective gene expressions in the post–hematopoietic transplant group. M edian glucagone like peptide-1 concentrations were significantly decreased in patients with features of acute graft versus host disease. Moreover, negative correlation between glucagone like peptide-1 concentrations and acute graft versus host disease severity was found. Increased concentrations and gene expressions of gastrointestinal tract regulation peptides can be caused by stimulation of regeneration in the severe injured organ. Measurement of these parameters may be a useful method of assessment of severity of gastrointestinal tract complications of hematopoietic stem cell transplantation.

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