Human 293 Cell Metabolism in Low Glutamine-Supplied Culture: Interpretation of Metabolic Changes through Metabolic Flux Analysis

2000 ◽  
Vol 2 (4) ◽  
pp. 277-292 ◽  
Author(s):  
I. Nadeau ◽  
J. Sabatié ◽  
M. Koehl ◽  
M. Perrier ◽  
A. Kamen
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Cell Metabolism ◽  
Metabolic Changes ◽  
Flux Analysis

Metabolic flux analysis of CHO cell metabolism in the late non-growth phase

2010 ◽  
Vol 108 (1) ◽  
pp. 82-92 ◽  
Author(s):  
Neelanjan Sengupta ◽  
Steven T. Rose ◽  
John A. Morgan
Keyword(s):  
Growth Phase ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Cell Metabolism ◽  
Flux Analysis ◽  
Cho Cell

scholarly journals Dynamic metabolic flux analysis using B-splines to study the effects of temperature shift on CHO cell metabolism

2015 ◽  
Vol 2 ◽  
pp. 46-57 ◽  
Author(s):  
Verónica S. Martínez ◽  
Maria Buchsteiner ◽  
Peter Gray ◽  
Lars K. Nielsen ◽  
Lake-Ee Quek
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Cell Metabolism ◽  
Temperature Shift ◽  
Flux Analysis ◽  
Cho Cell ◽  
B Splines ◽  
Effects Of Temperature

scholarly journals Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides

Glycobiology ◽  
2020 ◽  
Vol 30 (11) ◽  
pp. 859-871
Author(s):  
Maurice Wong ◽  
Gege Xu ◽  
Mariana Barboza ◽  
Izumi Maezawa ◽  
Lee-Way Jin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Differentiation ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Isotope Labeling ◽  
De Novo ◽  
Cell Metabolism ◽  
Flux Analysis ◽  
De Novo Synthesis ◽  
Direct Incorporation

Abstract Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans. Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well. Their influence on metabolism and how they are utilized have not been explored thoroughly. Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell. In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells. We gathered detailed isotopologue data for hundreds of glycoconjugates using mass spectrometry methods. The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation. Co-feeding studies revealed that fructose incorporation is drastically decreased by the presence of glucose, while mannose and galactose were much less affected. Furthermore, increased sialylation slowed down the turnover of glycans, but fucosylation attenuated this effect. Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures. We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.

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