CGSC: The E.coli Genetic Stock Center Database

2006 ◽  
pp. 175-183
Author(s):  
Mary K. B. Berlyn
Keyword(s):  
Genetic Stock

An Ultrastructural Comparison of Hepatocytes from ADH+ and ADH−Peromyscus Maniculatus

1996 ◽  
Vol 54 ◽  
pp. 30-31
Author(s):  
J. T. Ellzey ◽  
D. Borunda ◽  
B. P. Stewart
Keyword(s):  
South Carolina ◽  
Adult Male ◽  
Uranyl Acetate ◽  
Model System ◽  
Deer Mice ◽  
En Bloc ◽  
Genetic Stock ◽  
Hepes Buffer ◽  
Transmission Electron ◽  
Color Scanner

Genetically alcohol deficient deer mice (ADHN/ADHN) (obtained from the Peromyscus Genetic Stock Center, Univ. of South Carolina) lack hepatic cytosolic alcohol dehydrogenase. In order to determine if these deer mice would provide a model system for an ultrastructural study of the effects of ethanol on hepatocyte organelles, 75 micrographs of ADH+ adult male deer mice (n=5) were compared with 75 micrographs of ADH− adult male deer mice (n=5). A morphometric analysis of mitochondrial and peroxisomal parameters was undertaken.The livers were perfused with 0.1M HEPES buffer followed by 0.25% glutaraldehyde and 2% sucrose in 0.1M HEPES buffer (4C), removed, weighed and fixed by immersion in 2.5% glutaraldehyde in 0.1M HEPES buffer, pH 7.4, followed by a 3,3’ diaminobenzidine (DAB) incubation, postfixation with 2% OsO4, en bloc staining with 1% uranyl acetate in 0.025M maleate-NaOH buffer, dehydrated, embedded in Poly/Bed 812-BDMA epon resin, sectioned and poststained with uranyl acetate and lead citrate. Photographs were taken on a Zeiss EM-10 transmission electron microscope, scanned with a Howtek personal color scanner, analyzed with OPTIMAS 4.02 software on a Gateway2000 4DX2-66V personal computer and stored in Excel 4.0.

Multi-environment evaluation of Spanish bunch groundnut genotypes for fresh seed dormancy

2019 ◽  
Vol 79 (3) ◽  
Author(s):  
Narendra Kumar ◽  
B. C. Ajay ◽  
A. L. Rathnakumar ◽  
T. . Radhakrishnan ◽  
M. C. Dagla1 ◽  
...  
Keyword(s):  
Seed Dormancy ◽  
Stability Index ◽  
Pooled Analysis ◽  
Genetic Stock ◽  
Breeding Lines ◽  
Fresh Seed ◽  
Differential Behavior ◽  
Environmental Interactions ◽  
Crop Maturity

The present study was carried out to evaluate eight promising Spanish bunch groundnut genotypes during four consecutive year (2014, 2015, 2016, 2017) to study genotype × environmental interactions and to identify stable sources of fresh seed dormancy in Spanish background in groundnut. Pooled analysis of variance revealed highly significant differences among the genotypes and genotype × environmental interactions for fresh seed dormancy at weekly intervals indicating that differential behavior of genotypes for fresh seed dormancy over the environmental conditions. Based on the results of intensity and duration of dormancy and germination stability index, identified four stable advanced breeding lines viz., PBS 12192, PBS 12187, PBS 12191 and PBS 12190 having high fresh seed dormancy of three week and two stable advanced breeding lines viz., PBS 12189 and PBS 12171 having high fresh seed dormancy of two week. Therefore, these genotypes can be used as novel genetic stock of fresh seed dormancy in Spanish bunch and they can be integrated into breeding programs to develop high yielding Spanish bunch cultivars with 2-3 weeks of fresh seed dormancy to avoid yield losses due to in-situ germination at the time of crop maturity.

scholarly journals Genetic melting pot and importance of long-distance dispersal indicated in the Gladiolus imbricatus L. populations in the Polish Carpathians

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Agnieszka Sutkowska ◽  
Józef Mitka ◽  
Tomasz Warzecha ◽  
Jakub Bunk ◽  
Julia Rutkowska ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Diversity Indices ◽  
Western Carpathians ◽  
Genetic Structuring ◽  
Long Distance ◽  
Plant Populations ◽  
Genetic Stock ◽  
Melting Pot ◽  
Mountain Ranges ◽  
Eastern Carpathians

AbstractThe genetic diversity in 11 populations of Gladiolus imbricatus in five mountain ranges, including the Tatra, Pieniny, Gorce, Beskid Niski (Western Carpathians) and Bieszczady Mts (Eastern Carpathians), was studied with inter-simple sequence repeat (ISSR) markers. The species is a perennial plant occurring in open and semi-open sites of anthropogenic origin (meadows and forest margins). We checked a hypothesis on the microrefugial character of the plant populations in the Pieniny Mts, a small calcareous Carpathian range of complicated relief that has never been glaciated. Plant populations in the Tatra and Pieniny Mts had the highest genetic diversity indices, pointing to their long-term persistence. The refugial vs. the non-refugial mountain ranges accounted for a relatively high value of total genetic variation [analysis of molecular variance (AMOVA), 14.12%, p = 0.003]. One of the Pieniny populations was of hybridogenous origin and shared genetic stock with the Tatra population, indicating there is a local genetic melting pot. A weak genetic structuring of populations among particular regions was found (AMOVA, 4.5%, p > 0.05). This could be an effect of the frequent short-distance and sporadic long-distance gene flow. The dispersal of diaspores between the remote populations in the Western Carpathians and Eastern Carpathians could be affected by the historical transportation of flocks of sheep from the Tatra to Bieszczady Mts.

Comprehensive microsatellite baseline for genetic stock identification of Atlantic salmon (Salmo salar L.) in northernmost Europe

2017 ◽  
Vol 74 (8) ◽  
pp. 2159-2169 ◽  
Author(s):  
Mikhail Ozerov ◽  
Juha-Pekka Vähä ◽  
Vidar Wennevik ◽  
Eero Niemelä ◽  
Martin-A. Svenning ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Stock Identification ◽  
Genetic Stock ◽  
Atlantic Salmon Salmo Salar ◽  
Genetic Stock Identification

scholarly journals LASTING BIOLOGICAL EFFECTS OF EARLY ENVIRONMENTAL INFLUENCES

1968 ◽  
Vol 127 (4) ◽  
pp. 801-818 ◽  
Author(s):  
Egilde Seravalli ◽  
René Dubos
Keyword(s):  
Growth Rate ◽  
Mouse Embryo ◽  
Bacterial Infections ◽  
Biological Effects ◽  
Intestinal Flora ◽  
Adult Life ◽  
Tissue Cultures ◽  
Depressing Effect ◽  
Genetic Stock ◽  
Adult Weight

Certain specific-pathogen-free (SPF) mice bred and maintained under semiprotected conditions have an intestinal flora which is qualitatively simpler (although not quantitatively smaller) than that of mice of the same genetic stock produced under ordinary conditions. They are also heavier at weaning time, grow at a faster rate, and reach a greater adult weight than ordinary mice. When SPF mice are contaminated per os shortly after birth with certain bacterial cultures isolated from the intestinal contents of adult ordinary mice, these bacteria multiply extensively throughout the gastrointestinal tract and persist at extremely high levels until weaning time. Such bacterial infections do not affect significantly either weaning weight, growth rate, or maximum adult weight. In contrast, weight depression could be consistently brought about by contaminating newborn SPF mice per os with bacteria-free filtrates of homogenates of intestines from ordinary mice. The weight-depressing agent passed through Millipore discs of 0.45 and 0.22 µ porosity, but was held back at 0.10 µ porosity. The depression of weight caused by either intestine homogenate or filtrates thereof could be detected within a few days after contamination (of 2 day old mice) and persisted throughout the adult life of the contaminated animals. When intestine homogenate of the SPF mice used in this study were introduced per os into newborn SPF mice, they did not affect their growth rate or adult weight. On several occasions, but not consistently, bacteria-free filtrates capable of depressing the weight curve of SPF mice produced alterations in the appearance of tissue cultures of BHK-21 and mouse embryo cells. When tissue cultures so infected were introduced into newborn SPF mice, the weight of these animals was depressed early and lastingly. An agent exhibiting weight-depressing activity has been transferred from mouse to mouse over many passages by contaminating newborn SPF animals per os. Weight depression was achieved with extremely small doses of material (10–5 ml of intestine homogenate of 10–4 of mouse embryo culture). Under these conditions, none of the animals showed obvious signs of disease except reduced weight. Only very young SPF mice (preferably less than 3 days old) proved susceptible to the weight-depressing effect of the filtrates of intestine homogenates or of infected tissue cultures prepared therefrom. After oral contamination, it took approximately 1 wk before the intestinal homogenate obtained from contaminated animals exhibited a high level of weight-depressing activity. The growth-depressing effect could be transmitted from one generation to the next by mating SPF mice that had been contaminated shortly after birth and that were consequently smaller than control SPF animals.

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