High Throughput Production and Screening Strategies for Creating Advanced Biomaterials and Chemical Sensors

2009 ◽  
pp. 393-417
Author(s):  
William G. Holthoff ◽  
Loraine T. Tan ◽  
Ellen L. Holthoff ◽  
Ellen M. Cardone ◽  
Frank V. Bright
Keyword(s):  
High Throughput ◽  
Chemical Sensors ◽  
Screening Strategies ◽  
Advanced Biomaterials

Combinatorial Study and High-Throughput Screening of Transparent Barrier Films using Chemical Sensors

2003 ◽  
pp. 289-316
Author(s):  
Jaime C. Grunlan ◽  
Dennis Saunders ◽  
Jay Akhave ◽  
Mark Licon ◽  
Marcel Murga ◽  
...  
Keyword(s):  
High Throughput ◽  
Chemical Sensors ◽  
High Throughput Screening ◽  
Barrier Films ◽  
Transparent Barrier

Shadow Edge Lithography and Application to Nanofluidics

2010 ◽  
Author(s):  
Woon-Hong Yeo ◽  
Dong Won Lee ◽  
Kyong-Hoon Lee ◽  
Jae-Hyun Chung
Keyword(s):  
Fuel Cells ◽  
High Resolution ◽  
High Throughput ◽  
Single Molecule ◽  
Chemical Sensors ◽  
Geometric Distribution ◽  
High Vacuum ◽  
Vacuum Evaporation ◽  
Wafer Scale ◽  
Nanoscale Patterns

Many upcoming applications, such as nanoelectronic circuitry, single-molecule based chips, nanofluidics, chemical sensors, and fuel cells, require large arrays of nanochannels and nanowires. To commercialize such nanostructured devices, a high resolution and high throughput patterning method is essential. For this purpose, we developed the shadow edge lithography (SEL) as a wafer-scale, high-throughput nanomanufacturing method [1]. In the proposed method, the shadow effect in the high-vacuum evaporation was theoretically analyzed to predict the geometric distribution of the nanoscale patterns [2]. In experiment, nanoscale patterns were created by the shadow of aluminum (Al) edges that were prepatterned using a conventional microfabrication method.

scholarly journals High-throughput aminoacyl-tRNA synthetase engineering for genetic code expansion in yeast

2021 ◽  
Author(s):  
Jessica T. Stieglitz ◽  
James A. Van Deventer
Keyword(s):  
Genetic Code ◽  
High Throughput ◽  
Trna Synthetase ◽  
Noncanonical Amino Acids ◽  
Trna Synthetases ◽  
E Coli ◽  
Biological Studies ◽  
Screening Strategies ◽  
Genetic Code Expansion ◽  
Translation Systems

Protein expression with genetically encoded noncanonical amino acids (ncAAs) benefits a broad range of applications, from the discovery of biological therapeutics to fundamental biological studies. A major factor limiting the use of ncAAs is the lack of orthogonal translation systems (OTSs) that support efficient genetic code expansion at repurposed stop codons. Aminoacyl-tRNA synthetases (aaRSs) have been extensively evolved in E. coli but are not always orthogonal in eukaryotes. In this work, we use a yeast display-based ncAA incorporation reporter platform with fluorescence-activated cell sorting (FACS) to screen libraries of aaRSs in high throughput for 1) incorporation of ncAAs not previously encoded in yeast; 2) improvement of the performance of an existing aaRS; 3) highly selective OTSs capable of discriminating between closely related ncAA analogs; and 4) OTSs exhibiting enhanced polyspecificity to support translation with structurally diverse sets of ncAAs. The number of previously undiscovered aaRS variants we report in this work more than doubles the total number of translationally active aaRSs available for genetic code manipulation in yeast. The success of myriad screening strategies has important implications related to the fundamental properties and evolvability of aaRSs. Furthermore, access to OTSs with diverse activities and specific/polyspecific properties are invaluable for a range of applications within chemical biology, synthetic biology, and protein engineering.

scholarly journals Advances in Chirality Sensing with Macrocyclic Molecules

Chemosensors ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 279
Author(s):  
Xiaotong Liang ◽  
Wenting Liang ◽  
Pengyue Jin ◽  
Hongtao Wang ◽  
Wanhua Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Absolute Configuration ◽  
Chemical Sensors ◽  
Organic Molecules ◽  
Macrocyclic Compounds ◽  
Rapid Progress ◽  
Molecular Chirality ◽  
Supramolecular Chirality ◽  
Signal Output ◽  
Macrocyclic Molecules

The construction of chemical sensors that can distinguish molecular chirality has attracted increasing attention in recent years due to the significance of chiral organic molecules and the importance of detecting their absolute configuration and chiroptical purity. The supramolecular chirality sensing strategy has shown promising potential due to its advantages of high throughput, sensitivity, and fast chirality detection. This review focuses on chirality sensors based on macrocyclic compounds. Macrocyclic chirality sensors usually have inherent complexing ability towards certain chiral guests, which combined with the signal output components, could offer many unique advantages/properties compared to traditional chiral sensors. Chirality sensing based on macrocyclic sensors has shown rapid progress in recent years. This review summarizes recent advances in chirality sensing based on both achiral and chiral macrocyclic compounds, especially newly emerged macrocyclic molecules.

scholarly journals Stratified High-Throughput Screening Sets Enable Flexible Screening Strategies from a Single Plated Collection

2013 ◽  
Vol 19 (3) ◽  
pp. 369-378 ◽  
Author(s):  
J.Willem M. Nissink ◽  
Stefan Schmitt ◽  
Sam Blackburn ◽  
Stephen Peters
Keyword(s):  
Cost Saving ◽  
High Throughput ◽  
High Throughput Screening ◽  
Substantial Cost ◽  
Substantial Cost Saving ◽  
Set Size ◽  
Screening Strategies

Customized compound picking and plating of very large corporate screening decks (many 100,000s) for high-throughput screening is generally restricted, both from a time and cost perspective. Here we present a stratified screening deck with accompanying plating design for use with very large corporate compound collections. The deck is plated as a whole, but copies for screening can be downsized flexibly and quickly on the fly, without the need for repicking of physical samples. We show that such downsized sets maximize returns and yield results superior to randomly picked subsets of the same size. For the proposed stratified plating design, structurally diverse subsets that cover the full collection in terms of compound diversity and favorable compound properties can be produced economically and quickly from the full set of master plates. The design was implemented globally at AstraZeneca in 2009 and has enabled substantial cost-saving in screening campaigns, as set size requirements can be met on a per-screen basis, using a single, preplated master deck.

Application of Combinatorial Electropolymerization to the Development of Chemical Sensors

2003 ◽  
Vol 804 ◽  
Author(s):  
Vladimir M. Mirsky ◽  
Valentin Kulikov ◽  
Qingli Hao ◽  
Otto S. Wolfbeis
Keyword(s):  
Electrical Properties ◽  
Hydrogen Chloride ◽  
High Throughput ◽  
Chemical Sensors ◽  
Polymer Synthesis ◽  
Gaseous Hydrogen ◽  
Analytical Properties ◽  
Four Electrodes

ABSTRACTCombinatorial electropolymerization with electrical addressing was realized on the array of 96 electrode groups, each from four electrodes. The polymer synthesis was combined with subsequent high-throughput investigation of analytical properties of synthesized polymers. Two-and four-point techniques were applied simultaneously to measure electrical properties of synthesized polymers and contact resistances between electrodes and polymers and modifications of these parameters on addition of analyte. The system was used for development of sensitive materials for detection of gaseous hydrogen chloride.

Sign in / Sign up

Export Citation Format

Share Document