Genetic Mapping in the Triticeae

ABSTRACT The prlC gene of E. coli was originally identified as an allele, prlC1, which suppresses certain signal sequence mutations in the genes for several exported proteins. We have isolated six new alleles of prlC that also confer this phenotype. These mutations can be placed into three classes based on the degree to which they suppress the lamBsignal sequence deletion, lamBs78. Genetic mapping reveals that the physical location of the mutations in prlC correlates with the strength of the suppression, suggesting that different regions of the gene can be altered to yield a suppressor phenotype. We also describe an in vivo cloning procedure using λplacMu9H. The procedure relies on transposition and illegitimate recombination to generate a specialized transducing phage that carries prlC1. This method should be applicable to any gene for which there is a mutant phenotype.

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Human fatty acid synthase mRNA: tissue distribution, genetic mapping, and kinetics of decay after glucose deprivation

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)39738-8 ◽

1995 ◽

Vol 36 (7) ◽

pp. 1507-1521

Author(s):

C F Semenkovich ◽

T Coleman ◽

F T Fiedorek

Keyword(s):

Fatty Acid ◽

Genetic Mapping ◽

Tissue Distribution ◽

Fatty Acid Synthase ◽

Glucose Deprivation ◽

Mrna Tissue Distribution ◽

Kinetics Of

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GENETIC MAPPING OF GENES REQUIRED FOR MOTILITY IN CAULOBACTER CRESCENTUS

Genetics ◽

10.1093/genetics/108.3.523 ◽

1984 ◽

Vol 108 (3) ◽

pp. 523-532

Author(s):

Bert Ely ◽

Ronda H Croft ◽

Connie J Gerardot

Keyword(s):

Genetic Mapping ◽

Caulobacter Crescentus ◽

Pleiotropic Effect ◽

Structure And Function ◽

Bacteriophage Resistance ◽

And Function ◽

Chromosomal Map ◽

Bacterial Systems

ABSTRACT Mutations in more than 30 genes affect motility in Caulobacter crescentus. We have determined the chromosomal map locations for 27 genes involved in flagellar morphogenesis (fla), three genes involved in flagellar function (mot), and three genes that have a pleiotropic effect on both motility and bacteriophage resistance (ple). Three multigene clusters have been detected at widely separated chromosomal locations, but in addition, there are 12 fla and mot genes that are found at eight additional sites scattered around the C. cresentus chromosome. Thus, there is more scatter of genes involved in flagellar structure and function than has been observed in other bacterial systems.

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Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans

Scientific Reports ◽

10.1038/s41598-021-90190-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ho-Yon Hwang ◽

Jiou Wang

Keyword(s):

Caenorhabditis Elegans ◽

Genetic Mapping ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Genetic Material ◽

Mapping Method ◽

Forward Genetics ◽

Whole Genome ◽

Nucleotide Polymorphisms ◽

Large Populations

AbstractGenetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole-genome sequencing, mapping remains critical in pinpointing the responsible mutation. Here we describe a simple, fast, and affordable mapping toolkit that is particularly suitable for mapping in Caenorhabditis elegans. This mapping method uses insertion-deletion polymorphisms or indels that could be easily detected instead of single nucleotide polymorphisms in commonly used Hawaiian CB4856 mapping strain. The materials and methods were optimized so that mapping could be performed using tiny amount of genetic material without growing many large populations of mutants for DNA purification. We performed mapping of previously known and unknown mutations to show strengths and weaknesses of this method and to present examples of completed mapping. For situations where Hawaiian CB4856 is unsuitable, we provide an annotated list of indels as a basis for fast and easy mapping using other wild isolates. Finally, we provide rationale for using this mapping method over other alternatives as a part of a comprehensive strategy also involving whole-genome sequencing and other methods.

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Regulatory change in cell division activity and genetic mapping of a tomato (Solanum lycopersicum L.) elongated-fruit mutant

Plant Biotechnology ◽

10.5511/plantbiotechnology.14.0204a ◽

2014 ◽

Vol 31 (2) ◽

pp. 149-158 ◽

Cited By ~ 4

Author(s):

Katarut Chusreeaeom ◽

Tohru Ariizumi ◽

Erika Asamizu ◽

Yoshihiro Okabe ◽

Kenta Shirasawa ◽

...

Keyword(s):

Cell Division ◽

Genetic Mapping ◽

Solanum Lycopersicum ◽

Regulatory Change ◽

Solanum Lycopersicum L

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A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites.

Genetics ◽

10.1093/genetics/131.3.609 ◽

1992 ◽

Vol 131 (3) ◽

pp. 609-624 ◽

Cited By ~ 13

Author(s):

B D Williams ◽

B Schrank ◽

C Huynh ◽

R Shownkeen ◽

R H Waterston

Keyword(s):

Caenorhabditis Elegans ◽

Genetic Mapping ◽

Physical Map ◽

Nematode Caenorhabditis Elegans ◽

C Elegans ◽

Sequence Tagged Sites ◽

Mapping System ◽

Polymerase Chain ◽

Tc1 Element ◽

Single Reaction

Abstract We devised an efficient genetic mapping system in the nematode Caenorhabditis elegans which is based upon the differences in number and location of the transposable element Tc1 between the Bristol and Bergerac strains. Using the nearly completed physical map of the C. elegans genome, we selected 40 widely distributed sites which contain a Tc1 element in the Bergerac strain, but not in the Bristol strain. For each site a polymerase chain reaction assay was designed that can distinguish between the Bergerac Tc1-containing site and the Bristol "empty" site. By combining appropriate assays in a single reaction, one can score multiple sites within single worms. This permits a mutation to be rapidly mapped, first to a linkage group and then to a chromosomal subregion, through analysis of only a small number of progeny from a single interstrain cross.

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