Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes

2022 ◽  
pp. 37-46
Author(s):  
Brandt Warecki ◽  
Ian Bast ◽  
William Sullivan
Keyword(s):  
Cell Division ◽  
Live Imaging ◽  
Larval Brain

scholarly journals Continuous live imaging of adult neural stem cell division and lineage progression in vitro

Development ◽  
2011 ◽  
Vol 138 (6) ◽  
pp. 1057-1068 ◽  
Author(s):  
M. R. Costa ◽  
F. Ortega ◽  
M. S. Brill ◽  
R. Beckervordersandforth ◽  
C. Petrone ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Neural Stem Cell ◽  
Live Imaging ◽  
Adult Neural Stem Cell ◽  
Stem Cell Division

Live Imaging of Cell Shape During Cell Division

2017 ◽  
Vol 2017.52 (0) ◽  
pp. 187
Author(s):  
Yoshihiro Oda ◽  
Yohsuke Imai ◽  
Keiko Numayama-Tsuruta ◽  
Takuji Ishikawa
Keyword(s):  
Cell Division ◽  
Cell Shape ◽  
Live Imaging

scholarly journals Quantitative live-imaging of Aquilegia floral meristems reveals distinct patterns of floral organ initiation and cell-level dynamics of floral meristem termination

2021 ◽  
Author(s):  
Ya Min ◽  
Stephanie J. Conway ◽  
Elena M. Kramer
Keyword(s):  
Cell Division ◽  
Floral Organ ◽  
Live Imaging ◽  
Confocal Imaging ◽  
Floral Meristem ◽  
Flowering Plant ◽  
Molecular Networks ◽  
Floral Organs ◽  
Meristem Development ◽  
Floral Meristems

ABSTRACTIn-depth investigation of any developmental process in plants requires knowledge of both the underpinning molecular networks and how they directly determine patterns of cell division and expansion over time. Floral meristems (FM) produce floral organs, after which they undergo floral meristem termination (FMT), and precise control of organ initiation and FMT is crucial to reproductive success of any flowering plant. Using a live confocal imaging, we characterized developmental dynamics during floral organ primordia initiation and FMT in Aquilegia coerulea (Ranunculaceae). Our results have uncovered distinct patterns of primordium initiation between stamens and staminodes compared to carpels, and provided insight into the process of FMT, which is discernable based on cell division dynamics preceding carpel initiation. To our knowledge, this is the first quantitative live imaging of meristem development in a system with numerous whorls of floral organs as well as an apocarpous gynoecium. This study provides crucial information for our understanding of how the spatial-temporal regulation of floral meristem behavior is achieved in both an evolutionary and developmental context.

scholarly journals Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for Lis1/Dynactin in Spindle Assembly and Mitotic Checkpoint Control

2005 ◽  
Vol 16 (11) ◽  
pp. 5127-5140 ◽  
Author(s):  
Karsten H. Siller ◽  
Madeline Serr ◽  
Ruth Steward ◽  
Tom S. Hays ◽  
Chris Q. Doe
Keyword(s):  
Cell Cycle ◽  
Fluorescent Protein ◽  
Spindle Assembly ◽  
Mitotic Checkpoint ◽  
Live Imaging ◽  
Cell Cycle Checkpoint ◽  
Checkpoint Control ◽  
Larval Brain ◽  
Independent Functions

Lis1 is required for nuclear migration in fungi, cell cycle progression in mammals, and the formation of a folded cerebral cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactin-dependent processes is not clearly understood. Here we generate and/or characterize mutants for Drosophila Lis1 and a dynactin subunit, Glued, to investigate the role of Lis1/dynactin in mitotic checkpoint function. In addition, we develop an improved time-lapse video microscopy technique that allows live imaging of GFP-Lis1, GFP-Rod checkpoint protein, green fluorescent protein (GFP)-labeled chromosomes, or GFP-labeled mitotic spindle dynamics in neuroblasts within whole larval brain explants. Our mutant analyses show that Lis1/dynactin have at least two independent functions during mitosis: first promoting centrosome separation and bipolar spindle assembly during prophase/prometaphase, and subsequently generating interkinetochore tension and transporting checkpoint proteins off kinetochores during metaphase, thus promoting timely anaphase onset. Furthermore, we show that Lis1/dynactin/dynein physically associate and colocalize on centrosomes, spindle MTs, and kinetochores, and that regulation of Lis1/dynactin kinetochore localization in Drosophila differs from both Caenorhabditis elegans and mammals. We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release.

scholarly journals Development of the Middle Layer in the Anther of Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Jing-Shi Xue ◽  
Chi Yao ◽  
Qin-Lin Xu ◽  
Chang-Xu Sui ◽  
Xin-Lei Jia ◽  
...  
Keyword(s):  
Cell Division ◽  
Fluorescent Proteins ◽  
Middle Layer ◽  
Live Imaging ◽  
Anther Development ◽  
Anther Wall ◽  
Fundamental Information ◽  
Stage 4 ◽  
Cell Layers ◽  
Staining Methods

The middle layer is an essential cell layer of the anther wall located between the endothecium and tapetum in Arabidopsis. Based on sectioning, the middle layer was found to be degraded at stage 7, which led to the separation of the tapetum from the anther wall. Here, we established techniques for live imaging of the anther. We created a marker line with fluorescent proteins expressed in all anther layers to study anther development. Several staining methods were used in the intact anthers to study anther cell morphology. We clarified the initiation, development, and degradation of the middle layer in Arabidopsis. This layer is initiated from both the inner and outer secondary parietal cells at stage 4, stopped cell division at stage 6, and finally degraded at stage 11. The neighboring cell layers, the epidermis, and endothecium continued cell division until stage 10, which led to a thin middle layer. The degradation of the tapetum cell wall at stage 7 lead to its isolation from the anther wall. This work presents fundamental information on the development of the middle layer, which facilitates the further investigation of anther development and plant fertility. These live imaging methods could be useful in future studies.

scholarly journals Effects of caffeine on mitotic index in Drosophila prosaltans (Diptera)

1997 ◽  
Vol 20 (4) ◽  
pp. 655-658 ◽  
Author(s):  
Mary Massumi Itoyama ◽  
Hermione Elly Melara de Campos Bicudo ◽  
José Antônio Cordeiro
Keyword(s):  
Cell Division ◽  
Mitotic Index ◽  
Brain Cells ◽  
Larval Brain ◽  
And Control

The effect of two concentrations of caffeine (1500 <FONT FACE="Symbol">m</FONT>g/ml and 2500 <FONT FACE="Symbol">m</FONT>g/ml) on mitotic indices of Drosophila prosaltans was analyzed in larval brain cells. Although the differences detected between treated and control cells were not significant, the percentages obtained suggest a possible effect of caffeine in slowing the process of cell division

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