Preparing Yeast Suspension Through Serial Dilution for Enumeration

2021 ◽  
pp. 77-81
Author(s):  
Cíntia Lacerda Ramos ◽  
Karina Teixeira Magalhães-Guedes
Keyword(s):  
Serial Dilution ◽  
Yeast Suspension

scholarly journals Rinsing Condition Optimization of the Membrane Fouled by Yeast Suspension

2021 ◽  
Vol 647 ◽  
pp. 012164
Author(s):  
Yangyang Guo ◽  
Zhaoyu Qiao ◽  
Zhan Wang ◽  
Huijia Luo ◽  
Xi Wang
Keyword(s):  
Yeast Suspension ◽  
Condition Optimization

scholarly journals STUDIES ON THE BACTERIOPHAGE OF D'HERELLE

1925 ◽  
Vol 42 (4) ◽  
pp. 483-497 ◽  
Author(s):  
Jacques J. Bronfenbrenner ◽  
Charles Korb
Keyword(s):  
Quantitative Evaluation ◽  
Bacterial Species ◽  
Relative Concentration ◽  
Serial Dilution ◽  
The Other ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
High Concentration ◽  
Average Size ◽  
Broth Dilution

The experiments reported above confirm the fact that lytic principle is distributed in active solution in a state of indivisible units. This permits its quantitative evaluation by serial dilution, as well as by plating on agar. The latter method, however, often gives readings considerably lower than those obtained by the broth dilution method of titration. By varying the concentration of agar it has been possible to show that the discrepancy is due to adsorption of the lytic agent on agar. When the concentration of the latter is increased from 0.3 per cent to 2.5 per cent the number of plaques of lysis is reduced more than 100 times. At the same time the average size of the plaques also decreases approximately to one-tenth of the original. The size, as well as the number of plaques, has been found to depend also on the condition of the culture employed in titration. Thus, when the culture exposed to the action of lytic agent is composed of young susceptible bacteria, the greater the concentration of bacteria, the smaller the plaques. When the culture is composed partly of young and partly of old susceptible bacteria, both the size and the number of the plaques are diminished with the increase in the relative concentration of old bacteria. On the other hand, presence in the culture of resistant bacteria does not affect either the size or the number of the plaques so long as the relative concentration of susceptible bacteria in the culture is sufficient to allow formation of them. The plaques appearing in the presence of a high concentration of resistant variants in the culture are relatively indistinct owing to overgrowth. Under carefully controlled conditions the size of plaques is found to be determined by the character of the lytic filtrate. Thus in the case of lytic agents which act upon more than one bacterial species the size of the plaques remains constant, irrespective of the bacterial substratum used for the production of the active filtrate.

Effect of Disruption Methods on the Dead-End Microfiltration Behavior of Yeast Suspension

2010 ◽  
Vol 45 (8) ◽  
pp. 1042-1050 ◽  
Author(s):  
Dan Liu ◽  
Raphaëlle Savoire ◽  
Eugène Vorobiev ◽  
Jean-Louis Lanoisellé
Keyword(s):  
Yeast Suspension ◽  
Dead End ◽  
The Dead

Four-parameter paired response curve for serial dilution assays

2021 ◽  
pp. 1-16
Author(s):  
Youyi Fong ◽  
Sallie R. Permar ◽  
Georgia D. Tomaras
Keyword(s):  
Response Curve ◽  
Serial Dilution

scholarly journals Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity

2010 ◽  
Vol 52 (3) ◽  
pp. 139-143 ◽  
Author(s):  
Carolina Rodrigues Costa ◽  
Xisto Sena Passos ◽  
Lúcia Kioko Hasimoto e Souza ◽  
Percilia de Andrade Lucena ◽  
Orionalda de Fátima Lisboa Fernandes ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oral Cavity ◽  
Egg Yolk ◽  
Yeast Suspension ◽  
Phospholipase Activity ◽  
Candida Spp ◽  
Proteinase Production ◽  
Buccal Epithelial Cells ◽  
Different Sources ◽  
Adherence Ability

Phospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC) of 112 Candida isolates originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated. The proteinase production was detected by inoculation into bovine serum albumin (BSA) agar and the phospholipase activity was performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site.

scholarly journals ANTIBIOTICS RESISTANCE OF CORYNEBACTERIUM NON DIPHTHERIAE STRAINS

2017 ◽  
pp. 3-8
Author(s):  
G. G. Kharseeva ◽  
N. A. Voronina ◽  
T. D. Gasretova ◽  
O. I. Sylka ◽  
S. Yu. Tyukavkina
Keyword(s):  
Serial Dilution ◽  
Urogenital Tract ◽  
Antibiotics Resistance ◽  
Dilution Method ◽  
Frequency Of Occurrence ◽  
Serial Dilution Method ◽  
Resistant Strains

Aim. Study the frequency of occurrence of antibiotics resistant strains of various species of Corynebacterium non diptheriae. Materials and methods. C.pseudodiphtheriticum, C.pseudotuberculosis, C.xerosis, C.amycolatum, C.striatum, C.ulcerans strains isolated from patients with pathologies of respiratory and urogenital tract, as well as individuals taking prophylaxis examination were used. Sensitivity to antibacterial preparations was determined by the serial dilution method. Results. The highest number of Corynebacterium non diptheriae strains displayed resistance to benzylpenicillin (54.8%) and lincomycin (50.7%), and lowest - to cefotaxime, cefazolin (6.8%) and vancomycin (13.7%). The highest number of antibiotics resistant strains were detected among members of C.pseudotuberculosis {100%), C.xerosis (96.0%) and C. pseudodiphtheriticum (81.0%) species. Polyresistant strains were detected most frequently among C.xerosis, C.amycolatum and C.striatum species. Strains of Corynebacterium non diptheriae most frequently displayed resistance to 1 or 2 antibacterial preparations (24.7%), less frequently - to 3 (20.5%), 4 (13.7%), 5 (4.1%) and 6 (1.4%) preparations. Conclusion. The amount of antibiotics resistant strains of Corynebacterium non diptheriae is large (89.0%) and non-similar in various species.

Integrated Serial Dilution on a Microchip for Immunoassay Sample Treatment and Flow Injection Analysis

1998 ◽  
pp. 157-160 ◽  
Author(s):  
Siew Bang Cheng ◽  
Cameron D. Skinner ◽  
D. Jed Harrison
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Serial Dilution ◽  
Sample Treatment

Serial Dilution

SciVee ◽  
2010 ◽  
Keyword(s):  
Serial Dilution
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