Cell Manipulation with Robot-Aided Optical Tweezers Technology

2012 ◽  
pp. 159-174 ◽  
Author(s):  
Songyu Hu ◽  
Youhua Tan ◽  
Dong Sun
Keyword(s):  
Optical Tweezers ◽  
Cell Manipulation

The efficiency of fiber optical tweezers for cell manipulation using distinct fabrication methods

2015 ◽  
Author(s):  
R. S. Rodrigues Ribeiro ◽  
O. Soppera ◽  
J. Viegas ◽  
A. Guerreiro ◽  
P. A. S. Jorge
Keyword(s):  
Optical Tweezers ◽  
Cell Manipulation ◽  
Fiber Optical ◽  
Fiber Optical Tweezers

scholarly journals Automated Indirect Transportation of Biological Cells with Optical Tweezers and a 3D Printed Microtool

2019 ◽  
Vol 9 (14) ◽  
pp. 2883 ◽  
Author(s):  
Songyu Hu ◽  
Heng Xie ◽  
Tanyong Wei ◽  
Shuxun Chen ◽  
Dong Sun
Keyword(s):  
Laser Beam ◽  
Optical Tweezers ◽  
High Precision ◽  
Optical Tweezer ◽  
Cell Manipulation ◽  
Laser Exposure ◽  
Direct Writing ◽  
Negative Effects ◽  
Direct Exposure ◽  
Direct Cell

Optical tweezers are widely used for noninvasive and precise micromanipulation of living cells to understand biological processes. By focusing laser beams on cells, direct cell manipulation with optical tweezers can achieve high precision and flexibility. However, direct exposure to the laser beam can lead to negative effects on the cells. These phenomena are also known as photobleaching and photodamage. In this study, we proposed a new indirect cell micromanipulation approach combined with a robot-aided holographic optical tweezer system and 3D nano-printed microtool. The microtool was designed with a V-shaped head and an optical handle part. The V-shaped head can push and trap different sizes of cells as the microtool moves forward by optical trapping of the handle part. In this way, cell exposure to the laser beam can be effectively reduced. The microtool was fabricated with a laser direct writing system by two-photon photopolymerization. A control strategy combined with an imaging processing algorithm was introduced for automated manipulation of the microtool and cells. Experiments were performed to verify the effectiveness of our approach. First, automated microtool transportation and rotation were demonstrated with high precision. Second, indirect optical transportations of cells, with and without an obstacle, were performed to demonstrate the effectiveness of the proposed approach. Third, experiments of fluorescent cell manipulation were performed to confirm that, indicated by the photobleaching effect, indirect manipulation with the microtool could induce less laser exposure compared with direct optical manipulation. The proposed method could be useful in complex biomedical applications where precise cell manipulation and less laser exposure are required.

Using GPUs for Realtime Prediction of Optical Forces on Microsphere Ensembles

2012 ◽  
Author(s):  
Sujal Bista ◽  
Sagar Chowdhury ◽  
Satyandra K. Gupta ◽  
Amitabh Varshney
Keyword(s):  
Laser Beam ◽  
Optical Tweezers ◽  
Computational Models ◽  
Laser Beams ◽  
Cell Manipulation ◽  
Optical Traps ◽  
Optical Forces ◽  
Gaussian Laser Beam ◽  
Multiple Particles ◽  
Speed Up

Laser beams can be used to create optical traps that can hold and transport small particles. Optical trapping has been used in a number of applications ranging from prototyping at the microscale to biological cell manipulation. Successfully using optical tweezers requires predicting optical forces on the particle being trapped and transported. Reasonably accurate theory and computational models exist for predicting optical forces on a single particle in the close vicinity of a Gaussian laser beam. However, in practice the workspace includes multiple particles that are manipulated using individual optical traps. It has been experimentally shown that the presence of a particle can cast a shadow on a nearby particle and hence affect the optical forces acting on it. Computing optical forces in the presence of shadows in real-time is not feasible on CPUs. In this paper, we introduce a ray-tracing-based application optimized for GPUs to calculate forces exerted by the laser beams on microparticle ensembles in an optical tweezers system. When evaluating the force exerted by a laser beam on 32 interacting particles, our GPU-based application is able to get a 66-fold speed up compared to a single core CPU implementation of traditional Ashkin’s approach and a 10-fold speedup over its single core CPU-based counterpart.

Investigation of Automated Cell Manipulation in Optical Tweezers-Assisted Microfluidic Chamber Using Simulations

2011 ◽  
Author(s):  
Sagar Chowdhury ◽  
Petr Svec ◽  
Chenlu Wang ◽  
Kevin T. Seale ◽  
John P. Wikswo ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Optical Tweezers ◽  
High Precision ◽  
High Throughput ◽  
Microfluidic Devices ◽  
Cell Manipulation ◽  
Precise Control ◽  
Biological Objects ◽  
Microfluidic Chamber ◽  
Manipulation Process

Microfluidic devices are well suited for the study of biological objects because of their indirect nature of manipulation and high throughput. However, the cell manipulation process solely depends on the fluid flow and hence precise control is difficult to attain inside a microfluidic chamber. Utilizing optical tweezers as a complementary tool provides precise manipulation control. We have presented an automated cell manipulation approach using optical tweezers operating inside a microfluidic chamber. To test and demonstrate the effectiveness of the approach we have developed a physics-based simulator that is completely automated and allows high precision of manipulation.

scholarly journals Auxiliary Optomechanical Tools for 3D Cell Manipulation

Micromachines ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 90 ◽  
Author(s):  
Ivan Shishkin ◽  
Hen Markovich ◽  
Yael Roichman ◽  
Pavel Ginzburg
Keyword(s):  
Optical Tweezers ◽  
General Concept ◽  
Cell Manipulation ◽  
Pure Liquid ◽  
Biological Objects ◽  
3D Manipulation ◽  
Direct Laser ◽  
Out Of Plane ◽  
Recent Developments ◽  
The Way

Advances in laser and optoelectronic technologies have brought the general concept of optomechanical manipulation to the level of standard biophysical tools, paving the way towards controlled experiments and measurements of tiny mechanical forces. Recent developments in direct laser writing (DLW) have enabled the realization of new types of micron-scale optomechanical tools, capable of performing designated functions. Here we further develop the concept of DLW-fabricated optomechanically-driven tools and demonstrate full-3D manipulation capabilities over biological objects. In particular, we resolved the long-standing problem of out-of-plane rotation in a pure liquid, which was demonstrated on a living cell, clamped between a pair of forks, designed for efficient manipulation with holographic optical tweezers. The demonstrated concept paves the way for the realization of flexible tools for performing on-demand functions over biological objects, such as cell tomography and surgery to name just few.

Efficient PEGfusion Combinedoptical Tweezers and Dielectrophoresis

2014 ◽  
Vol 595 ◽  
pp. 61-64
Author(s):  
Yoshihiro Mizuta ◽  
Kozo Taguchi
Keyword(s):  
Polyethylene Glycol ◽  
Cell Fusion ◽  
Optical Tweezers ◽  
Target Cell ◽  
Cell Manipulation ◽  
Uniform Electric Field ◽  
Specific Cell ◽  
Red Cabbage ◽  
Non Invasive ◽  
Fusion Efficiency

Cell fusionis difficult so that research institutions try to fusion with many methods. For example, method of using polyethylene glycol (PEG) is useful and it mainly use in fusion. However cell fusion efficiency of this method is less. In this paper we suggest efficient fusion of PEG with combining optical tweezers and dielectrophoresis (DEP). Optical tweezers is useful tool in cell manipulation ant it has features of non-invasive and non-contact. Using this technique, we can take target cell from many cells. DEP are known to manipulate cell and form pearl chain by non-uniform electric field. We think DEP lead to efficient cell fusion of PEG because probability of cell adhered by only PEG is less.So we performed firstly take protoplast of red cabbage as specific cell from cells to parallel electrodes by optical tweezers and second, we observed cell-cell fusion by PEG with cell formed pearl chain by DEP. Furthermore we demonstrated using optical tweezers at 980 nm, showed manipulation dates of polymer microspheres, yeast cell and protoplast of red cabbage.

Automatic transportation of biological cells with a robot-tweezer manipulation system

2011 ◽  
Vol 30 (14) ◽  
pp. 1681-1694 ◽  
Author(s):  
Songyu Hu ◽  
Dong Sun
Keyword(s):  
Optical Tweezers ◽  
Single Cells ◽  
Optical Trap ◽  
Cell Interaction ◽  
Cell Manipulation ◽  
Live Cells ◽  
Biological Cells ◽  
Manipulation System ◽  
End Effectors ◽  
Multiple Cells

The positioning of biological cells has become increasingly important in biomedical research such as drug discovery, cell-to-cell interaction, and tissue engineering. Significant demand for both accuracy and productivity in cell manipulation highlights the need for automated cell transportation with integrated robotics and micro/nano-manipulation technologies. Optical tweezers, which use highly focused low-power laser beams to trap and manipulate particles at the micro/nanoscale, can be treated as special robot ‘end-effectors’ to manipulate biological objects in a noninvasive way. In this paper, we propose to use a robot-tweezer manipulation system for automatic transportation of biological cells. A dynamics equation of the cell in an optical trap is analyzed. Closed-loop controllers are designed for positioning single cells as well as multiple cells. A synchronization control technology is utilized for multicell transportation with maintained cell pattern. Experiments are performed on transporting live cells to demonstrate the effectiveness of the proposed approach.

Rapid and Direct Cell-to-Cell Adherence Using Avidin-Biotin Binding System: Large Aggregate Formation in Suspension Culture and Small Tissue Element Formation Having a Precise Microstructure Using Optical Tweezers

2010 ◽  
Vol 22 (5) ◽  
pp. 619-622 ◽  
Author(s):  
Nobuhiko Kojima ◽  
◽  
Ken Miura ◽  
Tomoki Matsuo ◽  
Hidenari Nakayama ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Single Cell ◽  
Optical Tweezers ◽  
Single Cells ◽  
Cell Manipulation ◽  
Cell Adherence ◽  
Intact Cells ◽  
Binding System ◽  
Biotin Binding ◽  
Single Cell Manipulation

Effectively organizing isolated cells to tissue elements having an appropriate microstructure is a fundamental issue in future tissue engineering, but biological cell-to-cell adhesion is too weak to assemble single cells directly. In order to overcome the difficulty, we applied an Avidin-Biotin Binding System (ABBS) to cell surfaces, and avidinylated and biotinylated cells could mutually bind in the short time they were mixed together. Unlike conventional intact cells, ABBS helped make larger spheroids. Interestingly, avidinylated and biotinylated cell adherence occurred within 1 sec using laser trapping, enabling single cell manipulation. We showed precise, direct single-cell-based tissue assembly using ABBS and optical tweezers, followed by damage-free tissue culture. The combination of ABBS and single cell manipulation has considerable potential for use in application such as tissue engineering, regenerative medicine, and drug screening system.

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